Bat coronavirus HKU4 (BtCoV) (BtCoV/HKU4/2004)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Merbecovirus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A3EX96|NS3B_BCHK4 Non-structural protein 3b OS=Bat coronavirus HKU4 OX=694007 GN=3b PE=3 SV=1
MM1 pKa = 7.34VSFNATAILLLLLANAFSKK20 pKa = 10.33PLYY23 pKa = 10.14VPEE26 pKa = 4.66HH27 pKa = 6.51CGGMSGTLFQACIRR41 pKa = 11.84QTMVDD46 pKa = 3.47TTGMYY51 pKa = 9.04TNSAMSHH58 pKa = 6.87DD59 pKa = 4.37GVTIPFDD66 pKa = 3.73RR67 pKa = 11.84DD68 pKa = 3.79GIVHH72 pKa = 6.91EE73 pKa = 4.39DD74 pKa = 3.99HH75 pKa = 6.3YY76 pKa = 10.19TEE78 pKa = 4.55TNPTPLFDD86 pKa = 5.08AGFSVV91 pKa = 4.1

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0C6T4|R1A_BCHK4 Replicase polyprotein 1a OS=Bat coronavirus HKU4 OX=694007 GN=1a PE=1 SV=1
MM1 pKa = 7.35ATPAAPRR8 pKa = 11.84TISFADD14 pKa = 3.62NNDD17 pKa = 3.4NQPNQQQRR25 pKa = 11.84GRR27 pKa = 11.84GRR29 pKa = 11.84NPKK32 pKa = 9.25PRR34 pKa = 11.84PAPNNTVSWYY44 pKa = 9.65TGLTQHH50 pKa = 6.52GKK52 pKa = 9.78NPLAFPPGQGVPLNANSTTAQNAGYY77 pKa = 9.31WRR79 pKa = 11.84RR80 pKa = 11.84QDD82 pKa = 3.96RR83 pKa = 11.84KK84 pKa = 9.42INTGNGVKK92 pKa = 10.0QLAPRR97 pKa = 11.84WFFYY101 pKa = 9.92YY102 pKa = 10.27TGTGPEE108 pKa = 3.84ANLPFRR114 pKa = 11.84SVKK117 pKa = 10.67DD118 pKa = 3.88GIVWVYY124 pKa = 11.15EE125 pKa = 4.21EE126 pKa = 5.03GATDD130 pKa = 3.58APSVFGTRR138 pKa = 11.84NPANDD143 pKa = 3.63AAIVCQFAPGTLIPKK158 pKa = 9.26NFHH161 pKa = 6.53IEE163 pKa = 4.18GTGGNSQSSSRR174 pKa = 11.84ASSNSRR180 pKa = 11.84NSSRR184 pKa = 11.84SSSRR188 pKa = 11.84GGRR191 pKa = 11.84STSNSRR197 pKa = 11.84GTSPVSHH204 pKa = 6.3GVGSAEE210 pKa = 4.25SLAALPLLLDD220 pKa = 3.75LQKK223 pKa = 11.08RR224 pKa = 11.84LADD227 pKa = 3.86LEE229 pKa = 4.54SGKK232 pKa = 10.61SKK234 pKa = 10.4QPKK237 pKa = 9.57VVTKK241 pKa = 10.49KK242 pKa = 10.56DD243 pKa = 2.83AAAAKK248 pKa = 10.32NKK250 pKa = 8.56MRR252 pKa = 11.84HH253 pKa = 5.39KK254 pKa = 10.51RR255 pKa = 11.84VATKK259 pKa = 10.64GFNVTQAFGLRR270 pKa = 11.84GPGPLQGNFGDD281 pKa = 3.68MNYY284 pKa = 10.59NKK286 pKa = 10.08FGTEE290 pKa = 3.93DD291 pKa = 3.63PRR293 pKa = 11.84WPQMAEE299 pKa = 3.91LAPSASAFMSMSQFKK314 pKa = 8.91LTHH317 pKa = 5.98QSNDD321 pKa = 3.46DD322 pKa = 3.85KK323 pKa = 11.68GDD325 pKa = 4.31PIYY328 pKa = 10.57FLSYY332 pKa = 10.6SGAIKK337 pKa = 10.63LDD339 pKa = 3.72PKK341 pKa = 10.55NPNYY345 pKa = 10.35KK346 pKa = 9.79KK347 pKa = 9.77WLEE350 pKa = 3.89LLEE353 pKa = 4.58SNIDD357 pKa = 3.4AYY359 pKa = 9.71KK360 pKa = 10.21TFPKK364 pKa = 10.18KK365 pKa = 9.25EE366 pKa = 4.1RR367 pKa = 11.84KK368 pKa = 9.31PKK370 pKa = 7.46TTEE373 pKa = 4.02DD374 pKa = 3.6GAVASSSASQMEE386 pKa = 4.42DD387 pKa = 2.37VDD389 pKa = 5.05AKK391 pKa = 10.18PQRR394 pKa = 11.84KK395 pKa = 8.32PKK397 pKa = 10.45SRR399 pKa = 11.84VAGSITMRR407 pKa = 11.84SGSLPALQDD416 pKa = 3.21VTFDD420 pKa = 3.85SEE422 pKa = 4.18AA423 pKa = 3.72

Molecular weight: 45.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

14351

82

7119

1435.1

159.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.073 ± 0.259

3.052 ± 0.292

5.477 ± 0.319

3.846 ± 0.182

4.885 ± 0.222

5.937 ± 0.256

1.93 ± 0.146

4.94 ± 0.285

5.372 ± 0.366

9.553 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.127

5.526 ± 0.142

3.944 ± 0.617

3.087 ± 0.388

3.589 ± 0.29

7.449 ± 0.719

6.759 ± 0.266

9.198 ± 0.855

1.178 ± 0.095

4.954 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski