Rosellinia necatrix victorivirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Victorivirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060N758|A0A060N758_9VIRU RNA-directed RNA polymerase OS=Rosellinia necatrix victorivirus 1 OX=1148491 GN=RdRp PE=3 SV=1
MM1 pKa = 7.69AATARR6 pKa = 11.84NNFLASVVANARR18 pKa = 11.84GQPITAAGHH27 pKa = 5.18RR28 pKa = 11.84RR29 pKa = 11.84FRR31 pKa = 11.84SVVRR35 pKa = 11.84TSATIGGVNDD45 pKa = 3.35SRR47 pKa = 11.84AASIFYY53 pKa = 10.34EE54 pKa = 3.92IGRR57 pKa = 11.84GVNSSEE63 pKa = 4.21SALAVPTDD71 pKa = 2.84ATMRR75 pKa = 11.84VEE77 pKa = 4.52AAYY80 pKa = 7.21PTNAVLAEE88 pKa = 4.36DD89 pKa = 4.38FVGLAKK95 pKa = 10.33KK96 pKa = 10.41YY97 pKa = 10.76SNFSSTFEE105 pKa = 3.96YY106 pKa = 11.01SSLAGVAEE114 pKa = 4.06RR115 pKa = 11.84LARR118 pKa = 11.84ALAARR123 pKa = 11.84SIWDD127 pKa = 4.42DD128 pKa = 3.63FDD130 pKa = 3.58STAITGGRR138 pKa = 11.84TVVVHH143 pKa = 7.22ALGTYY148 pKa = 9.6DD149 pKa = 4.07GPVNSMTDD157 pKa = 3.1TVFIPRR163 pKa = 11.84LTNTNVRR170 pKa = 11.84GDD172 pKa = 3.59VFAVLVAAVTGEE184 pKa = 4.11GSAVATDD191 pKa = 3.82VLEE194 pKa = 4.22VDD196 pKa = 3.32AVTRR200 pKa = 11.84QAVVPVVDD208 pKa = 5.74GVALPLALVDD218 pKa = 3.6ALRR221 pKa = 11.84IIGANMIASDD231 pKa = 3.96QGPLFALAVTRR242 pKa = 11.84GIHH245 pKa = 6.87RR246 pKa = 11.84ILSVVGHH253 pKa = 5.7TDD255 pKa = 2.76EE256 pKa = 6.05GGTTRR261 pKa = 11.84DD262 pKa = 3.9LLRR265 pKa = 11.84CAAFSPPFGGVSSRR279 pKa = 11.84LSDD282 pKa = 3.84YY283 pKa = 11.43AGLPALSTNGVSVVASYY300 pKa = 11.41VDD302 pKa = 4.27SIALVTAALVAHH314 pKa = 7.05SDD316 pKa = 3.99PGDD319 pKa = 3.73HH320 pKa = 6.81YY321 pKa = 11.04QGEE324 pKa = 4.33WFPTFFLGATPTDD337 pKa = 3.51GDD339 pKa = 4.02ARR341 pKa = 11.84PGQHH345 pKa = 5.89TAGTAAMAGAIRR357 pKa = 11.84AQLLNDD363 pKa = 4.55QEE365 pKa = 5.46RR366 pKa = 11.84FWQNYY371 pKa = 7.64CRR373 pKa = 11.84GLGMIFCAEE382 pKa = 4.25GASDD386 pKa = 3.69LAVRR390 pKa = 11.84FSVTASYY397 pKa = 11.43SLGADD402 pKa = 3.43CRR404 pKa = 11.84HH405 pKa = 6.07LRR407 pKa = 11.84HH408 pKa = 6.38ASVAPWFWIEE418 pKa = 4.05PTSLLPSTLLGTEE431 pKa = 4.25AEE433 pKa = 4.77SIGHH437 pKa = 6.52ASFGGRR443 pKa = 11.84DD444 pKa = 3.72SQSLLPAWEE453 pKa = 5.06AIVPAGGRR461 pKa = 11.84DD462 pKa = 3.6TTFSAYY468 pKa = 9.62HH469 pKa = 5.43VRR471 pKa = 11.84LPSARR476 pKa = 11.84RR477 pKa = 11.84AAMLTHH483 pKa = 6.82WLGHH487 pKa = 5.48EE488 pKa = 4.17LNGLGALSIRR498 pKa = 11.84QLDD501 pKa = 4.0PTTVVHH507 pKa = 6.82PGACAAHH514 pKa = 6.96PDD516 pKa = 3.26VRR518 pKa = 11.84DD519 pKa = 3.43RR520 pKa = 11.84VEE522 pKa = 3.83AALPITDD529 pKa = 4.11YY530 pKa = 11.29LWTRR534 pKa = 11.84GQSPFPAPGEE544 pKa = 3.9FLNLGATAGIFVRR557 pKa = 11.84HH558 pKa = 5.48ATFTDD563 pKa = 3.79DD564 pKa = 4.59GDD566 pKa = 3.37ITMTHH571 pKa = 6.64LPAARR576 pKa = 11.84EE577 pKa = 3.98MVNCAITIEE586 pKa = 4.2VGRR589 pKa = 11.84PLSIANGATNAADD602 pKa = 3.24ATARR606 pKa = 11.84RR607 pKa = 11.84ARR609 pKa = 11.84TRR611 pKa = 11.84GARR614 pKa = 11.84EE615 pKa = 3.66LVAVKK620 pKa = 10.04QRR622 pKa = 11.84AALFGVADD630 pKa = 4.0MAEE633 pKa = 4.22MPVMTSAPRR642 pKa = 11.84LDD644 pKa = 4.14RR645 pKa = 11.84APPPPPPGAPAAGHH659 pKa = 5.22GTGPGLMRR667 pKa = 11.84PPQQGVGAGPGTNEE681 pKa = 3.7PAGNALVPVGHH692 pKa = 6.97HH693 pKa = 6.52GATTAPPAPRR703 pKa = 11.84PAGPLGAMGGGGAPGGRR720 pKa = 11.84APPGGAPPPPPAGGPADD737 pKa = 4.96DD738 pKa = 5.69NNDD741 pKa = 3.47DD742 pKa = 4.77DD743 pKa = 5.84LRR745 pKa = 11.84PAPPVAVAAAPAEE758 pKa = 4.23RR759 pKa = 11.84AAEE762 pKa = 3.98QQ763 pKa = 3.2

Molecular weight: 78.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060N758|A0A060N758_9VIRU RNA-directed RNA polymerase OS=Rosellinia necatrix victorivirus 1 OX=1148491 GN=RdRp PE=3 SV=1
MM1 pKa = 7.55AALDD5 pKa = 3.44VRR7 pKa = 11.84AAEE10 pKa = 4.15FGLLGSHH17 pKa = 7.34LAMLIRR23 pKa = 11.84GVPGLSRR30 pKa = 11.84SYY32 pKa = 11.6DD33 pKa = 3.42DD34 pKa = 6.22LDD36 pKa = 3.58FTRR39 pKa = 11.84QLIRR43 pKa = 11.84LQSDD47 pKa = 3.79TSALARR53 pKa = 11.84DD54 pKa = 4.37HH55 pKa = 7.25PLLPCAVSLLLADD68 pKa = 5.32FPVQLAADD76 pKa = 4.07DD77 pKa = 4.53RR78 pKa = 11.84LTHH81 pKa = 6.52ALVNSAYY88 pKa = 10.57SSPAYY93 pKa = 10.54SLFPPEE99 pKa = 3.68IKK101 pKa = 10.31FRR103 pKa = 11.84IPGIKK108 pKa = 9.79HH109 pKa = 6.06SSTGNARR116 pKa = 11.84SYY118 pKa = 11.61ARR120 pKa = 11.84AALNMFIHH128 pKa = 6.38NADD131 pKa = 3.39LTEE134 pKa = 3.93AAFPRR139 pKa = 11.84KK140 pKa = 9.28AHH142 pKa = 6.15SAANDD147 pKa = 3.39KK148 pKa = 10.61PLLHH152 pKa = 6.83LRR154 pKa = 11.84GLLQGLRR161 pKa = 11.84TTQGTGAAALMVARR175 pKa = 11.84AAGRR179 pKa = 11.84LTEE182 pKa = 4.38DD183 pKa = 3.31QVISLILYY191 pKa = 7.56RR192 pKa = 11.84TALAPLFGQRR202 pKa = 11.84SYY204 pKa = 11.72DD205 pKa = 3.84YY206 pKa = 9.96AWWAITEE213 pKa = 4.3PGNTAGLSNALKK225 pKa = 10.71ALGLNATACGAALVEE240 pKa = 4.55AKK242 pKa = 10.27ALQGRR247 pKa = 11.84GIAPVDD253 pKa = 3.5MAGEE257 pKa = 4.24VAFRR261 pKa = 11.84VQPDD265 pKa = 3.54LVKK268 pKa = 11.08AKK270 pKa = 10.48VLEE273 pKa = 4.14PDD275 pKa = 3.16YY276 pKa = 11.67DD277 pKa = 3.73EE278 pKa = 4.95LRR280 pKa = 11.84EE281 pKa = 4.27HH282 pKa = 6.91IKK284 pKa = 10.65HH285 pKa = 5.59VLHH288 pKa = 6.96SEE290 pKa = 4.02LAGEE294 pKa = 5.09LEE296 pKa = 4.42LTPLAEE302 pKa = 4.55FWSSRR307 pKa = 11.84WLWCVNGSNTSTSDD321 pKa = 2.82RR322 pKa = 11.84AMGLDD327 pKa = 3.2PHH329 pKa = 7.08EE330 pKa = 4.72YY331 pKa = 10.8ADD333 pKa = 3.53THH335 pKa = 5.13TRR337 pKa = 11.84TYY339 pKa = 11.07RR340 pKa = 11.84RR341 pKa = 11.84MVAEE345 pKa = 4.14TLEE348 pKa = 4.04QEE350 pKa = 5.48PISTWDD356 pKa = 3.12GTTSVSASVKK366 pKa = 10.81LEE368 pKa = 4.04TAKK371 pKa = 10.7SRR373 pKa = 11.84AIFACDD379 pKa = 3.03TRR381 pKa = 11.84SYY383 pKa = 11.08FAFSWILNDD392 pKa = 3.54VQKK395 pKa = 10.79RR396 pKa = 11.84WGNNRR401 pKa = 11.84VLLDD405 pKa = 3.89PGKK408 pKa = 11.03GGMAGIDD415 pKa = 3.12RR416 pKa = 11.84RR417 pKa = 11.84IRR419 pKa = 11.84RR420 pKa = 11.84SQHH423 pKa = 4.68TGGVNVMLDD432 pKa = 3.4YY433 pKa = 11.58DD434 pKa = 4.74DD435 pKa = 5.9FNSHH439 pKa = 6.32HH440 pKa = 6.3STRR443 pKa = 11.84SMQILFEE450 pKa = 4.49EE451 pKa = 4.71LCDD454 pKa = 3.77MYY456 pKa = 11.14SAPSWYY462 pKa = 9.86RR463 pKa = 11.84DD464 pKa = 3.26TLVNSFEE471 pKa = 4.35RR472 pKa = 11.84MFINFEE478 pKa = 4.19GKK480 pKa = 7.36QHH482 pKa = 6.14HH483 pKa = 6.11VLGTLMSGHH492 pKa = 7.18RR493 pKa = 11.84GTTFINSVLNAVYY506 pKa = 9.95IRR508 pKa = 11.84AAIGGPTFDD517 pKa = 4.67SLVSLHH523 pKa = 6.48TGDD526 pKa = 5.29DD527 pKa = 3.76VYY529 pKa = 11.62LRR531 pKa = 11.84APTLSDD537 pKa = 3.44CSRR540 pKa = 11.84ILTAAKK546 pKa = 10.34DD547 pKa = 3.67YY548 pKa = 10.05GCRR551 pKa = 11.84MNPTKK556 pKa = 10.53QSIGFRR562 pKa = 11.84RR563 pKa = 11.84AEE565 pKa = 4.17FLRR568 pKa = 11.84MGIGEE573 pKa = 4.22RR574 pKa = 11.84ASYY577 pKa = 10.44GYY579 pKa = 10.31LARR582 pKa = 11.84SVAALACGNWFNPNPMTPWEE602 pKa = 4.51GFNSLVTSARR612 pKa = 11.84SIINRR617 pKa = 11.84AGGQLRR623 pKa = 11.84LGEE626 pKa = 4.35LLSPAVRR633 pKa = 11.84HH634 pKa = 5.92GKK636 pKa = 9.64SISYY640 pKa = 7.71RR641 pKa = 11.84TRR643 pKa = 11.84AQLIDD648 pKa = 3.91GEE650 pKa = 4.63CAIEE654 pKa = 4.05GAPVYY659 pKa = 10.88GSDD662 pKa = 3.61FRR664 pKa = 11.84LKK666 pKa = 9.46TFEE669 pKa = 4.15VKK671 pKa = 10.36LPKK674 pKa = 10.11ADD676 pKa = 3.81AVPISPRR683 pKa = 11.84WRR685 pKa = 11.84TNATRR690 pKa = 11.84DD691 pKa = 3.57YY692 pKa = 10.89LLDD695 pKa = 3.66HH696 pKa = 6.83VSPIEE701 pKa = 4.3AEE703 pKa = 4.14ALSLAGADD711 pKa = 3.39ITTMMVVASFGKK723 pKa = 10.2GLDD726 pKa = 3.36RR727 pKa = 11.84KK728 pKa = 9.79VLQSTPDD735 pKa = 3.22FVLRR739 pKa = 11.84RR740 pKa = 11.84KK741 pKa = 9.25PVRR744 pKa = 11.84AALGFTDD751 pKa = 4.61VVTLLTSIPRR761 pKa = 11.84VGCLSGYY768 pKa = 9.73PLLNLVAGRR777 pKa = 11.84LSNDD781 pKa = 3.4EE782 pKa = 4.22LRR784 pKa = 11.84HH785 pKa = 5.3LVLLAGGDD793 pKa = 3.61ANAADD798 pKa = 4.17LQVEE802 pKa = 4.29AFGAEE807 pKa = 4.02SLTRR811 pKa = 11.84NIIGTLPFSDD821 pKa = 3.77AVNLGKK827 pKa = 9.08RR828 pKa = 11.84TNSANVYY835 pKa = 8.94VLTSIYY841 pKa = 9.64MM842 pKa = 3.72

Molecular weight: 92.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1605

763

842

802.5

85.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.832 ± 1.813

0.997 ± 0.142

5.607 ± 0.07

3.801 ± 0.266

3.551 ± 0.097

8.91 ± 1.065

2.617 ± 0.003

3.801 ± 0.354

1.682 ± 0.872

9.907 ± 1.559

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.056 ± 0.15

3.489 ± 0.321

6.231 ± 1.37

2.118 ± 0.103

7.414 ± 0.228

6.854 ± 0.646

6.667 ± 0.366

6.916 ± 0.818

1.246 ± 0.134

2.305 ± 0.495
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski