Capybara microvirus Cap1_SP_81
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W496|A0A4P8W496_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_81 OX=2584792 PE=4 SV=1
MM1 pKa = 7.38 SLSKK5 pKa = 9.94 TIGGDD10 pKa = 3.04 RR11 pKa = 11.84 LGAGNKK17 pKa = 9.1 QKK19 pKa = 10.51 VHH21 pKa = 4.94 LHH23 pKa = 5.14 NYY25 pKa = 7.97 EE26 pKa = 3.87 RR27 pKa = 11.84 ATFNLDD33 pKa = 2.88 KK34 pKa = 10.56 VVRR37 pKa = 11.84 MSMAPGILYY46 pKa = 9.07 PFYY49 pKa = 11.21 QNVALLGDD57 pKa = 3.5 TWDD60 pKa = 3.5 ININSLVRR68 pKa = 11.84 TIPTIGPLFGTFKK81 pKa = 9.94 MQIDD85 pKa = 3.98 FFFYY89 pKa = 10.31 PIRR92 pKa = 11.84 LAQGLLHH99 pKa = 6.65 NNPLNLGMEE108 pKa = 4.22 TDD110 pKa = 4.34 KK111 pKa = 11.27 IKK113 pKa = 10.92 LPQIEE118 pKa = 4.97 IEE120 pKa = 4.03 TDD122 pKa = 3.36 TQKK125 pKa = 10.81 SEE127 pKa = 4.51 RR128 pKa = 11.84 NGNNWQINQSSLAAYY143 pKa = 10.09 LGIRR147 pKa = 11.84 GIGAPTWAEE156 pKa = 3.55 QSGEE160 pKa = 4.25 IIWVNPTIKK169 pKa = 10.31 RR170 pKa = 11.84 KK171 pKa = 9.85 FNATTFLAYY180 pKa = 10.32 ADD182 pKa = 3.88 IFKK185 pKa = 10.61 SYY187 pKa = 10.34 YY188 pKa = 10.53 ANKK191 pKa = 10.06 QEE193 pKa = 4.27 DD194 pKa = 3.52 NAYY197 pKa = 10.88 VIGQEE202 pKa = 4.14 LGKK205 pKa = 9.6 EE206 pKa = 4.09 HH207 pKa = 7.16 QEE209 pKa = 3.99 IPDD212 pKa = 3.64 WLINNYY218 pKa = 9.71 LFSFGNNNGQYY229 pKa = 10.65 CYY231 pKa = 11.18 LNWNDD236 pKa = 5.25 NEE238 pKa = 4.43 KK239 pKa = 10.02 QWEE242 pKa = 4.52 CNLQFAPTYY251 pKa = 10.04 IKK253 pKa = 10.74 YY254 pKa = 10.28 EE255 pKa = 4.12 RR256 pKa = 11.84 FDD258 pKa = 3.86 KK259 pKa = 11.01 DD260 pKa = 3.11 HH261 pKa = 6.9 SALEE265 pKa = 4.11 VNMWLMSRR273 pKa = 11.84 ISITTKK279 pKa = 9.95 GKK281 pKa = 8.43 TYY283 pKa = 10.85 KK284 pKa = 10.62 LPEE287 pKa = 5.07 LINLEE292 pKa = 4.3 VISDD296 pKa = 4.74 KK297 pKa = 10.52 ITITTPSEE305 pKa = 3.83 YY306 pKa = 10.19 EE307 pKa = 3.75 FYY309 pKa = 11.28 GNYY312 pKa = 8.42 VQYY315 pKa = 10.8 HH316 pKa = 7.1 IINNSIFGGDD326 pKa = 3.74 TIVEE330 pKa = 4.31 TNTYY334 pKa = 11.0 NEE336 pKa = 4.05 TSKK339 pKa = 11.17 LKK341 pKa = 11.08 LIDD344 pKa = 4.22 FPLKK348 pKa = 10.9 NIDD351 pKa = 3.42 DD352 pKa = 3.76 ARR354 pKa = 11.84 YY355 pKa = 9.71 IILGHH360 pKa = 5.5 NQPNEE365 pKa = 3.83 ALSINEE371 pKa = 4.64 DD372 pKa = 4.22 LNFLPYY378 pKa = 10.31 SQWSGKK384 pKa = 7.22 DD385 pKa = 3.05 TKK387 pKa = 11.21 NRR389 pKa = 11.84 TNNYY393 pKa = 7.31 WPHH396 pKa = 6.22 NGLFIKK402 pKa = 9.62 TYY404 pKa = 10.51 QSDD407 pKa = 4.18 LFNNWVKK414 pKa = 10.37 TDD416 pKa = 5.22 FITGDD421 pKa = 3.24 NGIQEE426 pKa = 4.06 ITKK429 pKa = 9.83 IDD431 pKa = 3.72 TTDD434 pKa = 3.38 GLILDD439 pKa = 5.02 EE440 pKa = 5.15 LNLMQKK446 pKa = 9.59 VYY448 pKa = 11.57 NMLNRR453 pKa = 11.84 VAISGGTYY461 pKa = 9.73 EE462 pKa = 5.9 DD463 pKa = 4.18 YY464 pKa = 11.64 LEE466 pKa = 4.17 VQYY469 pKa = 11.15 GVSALRR475 pKa = 11.84 LEE477 pKa = 4.27 EE478 pKa = 4.07 TPVYY482 pKa = 10.02 IGGYY486 pKa = 6.15 STEE489 pKa = 3.93 IDD491 pKa = 3.71 FEE493 pKa = 4.58 EE494 pKa = 4.64 VTSNAASNATGEE506 pKa = 4.15 IQNLGSLAGKK516 pKa = 9.85 GINRR520 pKa = 11.84 SPKK523 pKa = 9.91 GGRR526 pKa = 11.84 ISYY529 pKa = 9.46 KK530 pKa = 9.14 PKK532 pKa = 10.17 EE533 pKa = 3.9 IGFIMGICSLTPRR546 pKa = 11.84 IDD548 pKa = 3.41 YY549 pKa = 10.75 SQGNKK554 pKa = 7.84 FWEE557 pKa = 4.47 TEE559 pKa = 3.79 IEE561 pKa = 4.21 TLADD565 pKa = 3.3 FHH567 pKa = 7.77 VPAMDD572 pKa = 5.16 GIGYY576 pKa = 9.56 QDD578 pKa = 5.96 LITEE582 pKa = 3.94 QMAYY586 pKa = 10.36 FDD588 pKa = 4.42 TLILPDD594 pKa = 3.8 GEE596 pKa = 4.63 IIKK599 pKa = 10.47 HH600 pKa = 5.01 SAGKK604 pKa = 9.87 IPAWLNYY611 pKa = 6.66 MTDD614 pKa = 3.13 VNEE617 pKa = 4.49 TYY619 pKa = 11.45 GNFADD624 pKa = 3.94 NDD626 pKa = 3.9 EE627 pKa = 4.81 TSGQMWMTLNRR638 pKa = 11.84 NYY640 pKa = 10.56 EE641 pKa = 4.03 RR642 pKa = 11.84 TNDD645 pKa = 4.33 GIGDD649 pKa = 4.18 LTTYY653 pKa = 10.58 IDD655 pKa = 3.63 PTKK658 pKa = 10.63 YY659 pKa = 10.59 NYY661 pKa = 10.92 AFAVKK666 pKa = 10.23 DD667 pKa = 3.72 LEE669 pKa = 4.43 AQNFWAQIGMNIVARR684 pKa = 11.84 RR685 pKa = 11.84 KK686 pKa = 9.14 MSAKK690 pKa = 10.1 QIPNLL695 pKa = 3.74
Molecular weight: 79.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.835
IPC2_protein 4.927
IPC_protein 4.889
Toseland 4.774
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.991
Wikipedia 4.711
Rodwell 4.749
Grimsley 4.685
Solomon 4.851
Lehninger 4.8
Nozaki 4.952
DTASelect 5.118
Thurlkill 4.762
EMBOSS 4.749
Sillero 5.029
Patrickios 3.656
IPC_peptide 4.851
IPC2_peptide 5.016
IPC2.peptide.svr19 4.987
Protein with the highest isoelectric point:
>tr|A0A4P8W7L2|A0A4P8W7L2_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_81 OX=2584792 PE=4 SV=1
MM1 pKa = 7.62 CLYY4 pKa = 10.06 PRR6 pKa = 11.84 TIRR9 pKa = 11.84 NPRR12 pKa = 11.84 FAVGNKK18 pKa = 9.45 NGYY21 pKa = 8.55 SQDD24 pKa = 3.48 DD25 pKa = 3.89 CTDD28 pKa = 2.97 KK29 pKa = 11.16 RR30 pKa = 11.84 KK31 pKa = 10.32 LFIKK35 pKa = 9.8 IDD37 pKa = 3.64 CGNCEE42 pKa = 3.6 EE43 pKa = 4.82 CKK45 pKa = 10.06 RR46 pKa = 11.84 KK47 pKa = 9.67 RR48 pKa = 11.84 GKK50 pKa = 8.52 WWTIRR55 pKa = 11.84 IMEE58 pKa = 4.28 EE59 pKa = 3.66 LKK61 pKa = 10.7 VNKK64 pKa = 8.83 GTFVTLTFSAHH75 pKa = 6.83 SLLQLTKK82 pKa = 10.9 GEE84 pKa = 4.24 KK85 pKa = 9.66 TPTANEE91 pKa = 4.01 TARR94 pKa = 11.84 KK95 pKa = 8.41 AVRR98 pKa = 11.84 RR99 pKa = 11.84 FLEE102 pKa = 4.38 RR103 pKa = 11.84 YY104 pKa = 7.03 RR105 pKa = 11.84 KK106 pKa = 10.06 KK107 pKa = 9.62 YY108 pKa = 9.05 GHH110 pKa = 6.42 SIKK113 pKa = 10.49 HH114 pKa = 5.2 ILITEE119 pKa = 4.63 LGDD122 pKa = 4.64 DD123 pKa = 4.22 EE124 pKa = 5.51 NEE126 pKa = 4.0 HH127 pKa = 6.25 TGRR130 pKa = 11.84 IHH132 pKa = 6.54 LHH134 pKa = 6.43 GILFDD139 pKa = 4.58 TITKK143 pKa = 10.59 DD144 pKa = 3.03 EE145 pKa = 5.37 LYY147 pKa = 11.07 NLWQYY152 pKa = 11.66 GYY154 pKa = 10.33 VDD156 pKa = 3.24 TGSYY160 pKa = 10.45 CNEE163 pKa = 3.41 RR164 pKa = 11.84 TANYY168 pKa = 5.37 ITKK171 pKa = 10.37 YY172 pKa = 8.01 ITKK175 pKa = 9.91 LDD177 pKa = 3.97 TKK179 pKa = 10.59 HH180 pKa = 6.83 PEE182 pKa = 3.81 YY183 pKa = 9.85 KK184 pKa = 10.17 QKK186 pKa = 10.74 IFISAGLGKK195 pKa = 10.39 NYY197 pKa = 10.25 VEE199 pKa = 3.95 TAKK202 pKa = 10.45 RR203 pKa = 11.84 QKK205 pKa = 10.47 KK206 pKa = 9.02 KK207 pKa = 10.66 VYY209 pKa = 9.93 RR210 pKa = 11.84 FTNGTQFPLPTYY222 pKa = 9.76 YY223 pKa = 10.46 KK224 pKa = 9.96 NKK226 pKa = 9.54 VFTEE230 pKa = 4.22 EE231 pKa = 4.18 EE232 pKa = 4.3 RR233 pKa = 11.84 EE234 pKa = 4.15 QMWTDD239 pKa = 3.37 EE240 pKa = 4.28 LNKK243 pKa = 10.2 NEE245 pKa = 4.46 HH246 pKa = 6.88 YY247 pKa = 9.61 ITGITYY253 pKa = 9.14 RR254 pKa = 11.84 YY255 pKa = 7.68 RR256 pKa = 11.84 AGVIGIKK263 pKa = 10.42 GKK265 pKa = 8.67 EE266 pKa = 3.79 LEE268 pKa = 4.02 QARR271 pKa = 11.84 RR272 pKa = 11.84 EE273 pKa = 4.14 NEE275 pKa = 4.11 TRR277 pKa = 11.84 KK278 pKa = 9.41 HH279 pKa = 4.86 AQIANEE285 pKa = 3.81 NKK287 pKa = 10.49 GYY289 pKa = 11.3 GNTKK293 pKa = 7.83 KK294 pKa = 9.29 TKK296 pKa = 10.19 DD297 pKa = 3.31 VLINEE302 pKa = 4.46 KK303 pKa = 9.57 MLQNSQKK310 pKa = 10.68 EE311 pKa = 4.22 EE312 pKa = 4.12 QKK314 pKa = 11.33 DD315 pKa = 3.53 KK316 pKa = 11.67
Molecular weight: 37.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.879
IPC2_protein 8.946
IPC_protein 8.814
Toseland 9.589
ProMoST 9.355
Dawson 9.838
Bjellqvist 9.545
Wikipedia 10.014
Rodwell 10.218
Grimsley 9.911
Solomon 9.867
Lehninger 9.823
Nozaki 9.663
DTASelect 9.516
Thurlkill 9.692
EMBOSS 10.014
Sillero 9.78
Patrickios 5.181
IPC_peptide 9.853
IPC2_peptide 8.112
IPC2.peptide.svr19 8.002
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1622
75
695
270.3
30.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.597 ± 1.211
0.863 ± 0.411
5.117 ± 0.577
7.275 ± 1.004
3.268 ± 0.598
7.707 ± 1.484
1.665 ± 0.395
7.522 ± 1.342
7.707 ± 1.602
7.398 ± 0.651
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.466 ± 0.423
8.57 ± 0.491
2.713 ± 0.642
4.932 ± 0.577
4.254 ± 0.857
4.501 ± 0.929
7.213 ± 0.751
3.329 ± 0.348
1.85 ± 0.264
5.055 ± 0.669
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here