Amino acid dipepetide frequency for Mycobacterium phage Wanda

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.405AlaAla: 10.405 ± 0.725
1.333AlaCys: 1.333 ± 0.201
6.058AlaAsp: 6.058 ± 0.444
7.42AlaGlu: 7.42 ± 0.509
3.536AlaPhe: 3.536 ± 0.346
7.217AlaGly: 7.217 ± 0.534
2.319AlaHis: 2.319 ± 0.335
4.956AlaIle: 4.956 ± 0.446
4.029AlaLys: 4.029 ± 0.378
8.463AlaLeu: 8.463 ± 0.59
3.42AlaMet: 3.42 ± 0.357
2.956AlaAsn: 2.956 ± 0.344
4.29AlaPro: 4.29 ± 0.414
2.84AlaGln: 2.84 ± 0.328
6.608AlaArg: 6.608 ± 0.501
5.333AlaSer: 5.333 ± 0.388
5.391AlaThr: 5.391 ± 0.463
6.0AlaVal: 6.0 ± 0.456
1.826AlaTrp: 1.826 ± 0.23
2.319AlaTyr: 2.319 ± 0.23
0.0AlaXaa: 0.0 ± 0.0
Cys
0.985CysAla: 0.985 ± 0.184
0.145CysCys: 0.145 ± 0.071
1.275CysAsp: 1.275 ± 0.215
1.072CysGlu: 1.072 ± 0.208
0.377CysPhe: 0.377 ± 0.109
2.232CysGly: 2.232 ± 0.289
0.348CysHis: 0.348 ± 0.099
0.551CysIle: 0.551 ± 0.129
0.696CysLys: 0.696 ± 0.157
1.188CysLeu: 1.188 ± 0.21
0.261CysMet: 0.261 ± 0.085
0.667CysAsn: 0.667 ± 0.168
0.754CysPro: 0.754 ± 0.174
0.406CysGln: 0.406 ± 0.124
1.043CysArg: 1.043 ± 0.201
0.696CysSer: 0.696 ± 0.147
0.812CysThr: 0.812 ± 0.135
0.841CysVal: 0.841 ± 0.158
0.29CysTrp: 0.29 ± 0.089
0.348CysTyr: 0.348 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
5.71AspAla: 5.71 ± 0.428
0.927AspCys: 0.927 ± 0.179
3.884AspAsp: 3.884 ± 0.363
4.927AspGlu: 4.927 ± 0.525
2.058AspPhe: 2.058 ± 0.224
6.695AspGly: 6.695 ± 0.426
1.913AspHis: 1.913 ± 0.281
3.246AspIle: 3.246 ± 0.286
2.29AspLys: 2.29 ± 0.302
5.159AspLeu: 5.159 ± 0.405
1.072AspMet: 1.072 ± 0.182
2.232AspAsn: 2.232 ± 0.266
3.768AspPro: 3.768 ± 0.362
1.855AspGln: 1.855 ± 0.191
3.971AspArg: 3.971 ± 0.333
2.58AspSer: 2.58 ± 0.314
3.101AspThr: 3.101 ± 0.296
3.942AspVal: 3.942 ± 0.376
2.058AspTrp: 2.058 ± 0.231
2.58AspTyr: 2.58 ± 0.263
0.0AspXaa: 0.0 ± 0.0
Glu
6.782GluAla: 6.782 ± 0.498
1.304GluCys: 1.304 ± 0.252
3.42GluAsp: 3.42 ± 0.341
5.246GluGlu: 5.246 ± 0.509
3.13GluPhe: 3.13 ± 0.278
4.521GluGly: 4.521 ± 0.388
1.217GluHis: 1.217 ± 0.185
4.116GluIle: 4.116 ± 0.372
2.869GluLys: 2.869 ± 0.386
6.521GluLeu: 6.521 ± 0.44
2.261GluMet: 2.261 ± 0.274
1.913GluAsn: 1.913 ± 0.23
2.782GluPro: 2.782 ± 0.324
2.753GluGln: 2.753 ± 0.277
5.304GluArg: 5.304 ± 0.448
3.362GluSer: 3.362 ± 0.327
3.536GluThr: 3.536 ± 0.294
4.029GluVal: 4.029 ± 0.339
1.304GluTrp: 1.304 ± 0.215
2.551GluTyr: 2.551 ± 0.255
0.0GluXaa: 0.0 ± 0.0
Phe
2.667PheAla: 2.667 ± 0.292
0.464PheCys: 0.464 ± 0.111
2.377PheAsp: 2.377 ± 0.269
2.174PheGlu: 2.174 ± 0.224
1.13PhePhe: 1.13 ± 0.2
3.536PheGly: 3.536 ± 0.481
0.667PheHis: 0.667 ± 0.159
1.304PheIle: 1.304 ± 0.175
1.188PheLys: 1.188 ± 0.177
2.29PheLeu: 2.29 ± 0.247
0.87PheMet: 0.87 ± 0.146
1.507PheAsn: 1.507 ± 0.264
1.884PhePro: 1.884 ± 0.283
0.783PheGln: 0.783 ± 0.167
1.855PheArg: 1.855 ± 0.228
1.681PheSer: 1.681 ± 0.209
2.319PheThr: 2.319 ± 0.259
2.348PheVal: 2.348 ± 0.26
0.638PheTrp: 0.638 ± 0.143
0.754PheTyr: 0.754 ± 0.142
0.0PheXaa: 0.0 ± 0.0
Gly
6.985GlyAla: 6.985 ± 0.601
1.333GlyCys: 1.333 ± 0.224
5.797GlyAsp: 5.797 ± 0.456
5.333GlyGlu: 5.333 ± 0.422
3.449GlyPhe: 3.449 ± 0.405
9.101GlyGly: 9.101 ± 1.499
2.116GlyHis: 2.116 ± 0.288
3.797GlyIle: 3.797 ± 0.341
3.275GlyLys: 3.275 ± 0.352
7.478GlyLeu: 7.478 ± 0.594
2.348GlyMet: 2.348 ± 0.267
3.217GlyAsn: 3.217 ± 0.33
3.884GlyPro: 3.884 ± 0.294
2.551GlyGln: 2.551 ± 0.342
5.565GlyArg: 5.565 ± 0.36
4.956GlySer: 4.956 ± 0.485
5.565GlyThr: 5.565 ± 0.454
5.71GlyVal: 5.71 ± 0.421
2.029GlyTrp: 2.029 ± 0.262
3.101GlyTyr: 3.101 ± 0.286
0.0GlyXaa: 0.0 ± 0.0
His
1.594HisAla: 1.594 ± 0.209
0.493HisCys: 0.493 ± 0.119
1.594HisAsp: 1.594 ± 0.237
1.768HisGlu: 1.768 ± 0.204
0.493HisPhe: 0.493 ± 0.14
2.116HisGly: 2.116 ± 0.247
0.956HisHis: 0.956 ± 0.185
0.956HisIle: 0.956 ± 0.189
0.725HisLys: 0.725 ± 0.153
2.319HisLeu: 2.319 ± 0.254
0.58HisMet: 0.58 ± 0.144
0.551HisAsn: 0.551 ± 0.129
1.333HisPro: 1.333 ± 0.188
0.812HisGln: 0.812 ± 0.134
1.826HisArg: 1.826 ± 0.254
0.87HisSer: 0.87 ± 0.135
0.87HisThr: 0.87 ± 0.173
1.304HisVal: 1.304 ± 0.202
0.696HisTrp: 0.696 ± 0.157
0.783HisTyr: 0.783 ± 0.168
0.0HisXaa: 0.0 ± 0.0
Ile
5.942IleAla: 5.942 ± 0.406
0.522IleCys: 0.522 ± 0.115
3.652IleAsp: 3.652 ± 0.331
4.0IleGlu: 4.0 ± 0.33
1.043IlePhe: 1.043 ± 0.203
4.0IleGly: 4.0 ± 0.399
1.159IleHis: 1.159 ± 0.189
1.855IleIle: 1.855 ± 0.246
1.565IleLys: 1.565 ± 0.203
3.681IleLeu: 3.681 ± 0.369
0.754IleMet: 0.754 ± 0.14
1.826IleAsn: 1.826 ± 0.221
2.811IlePro: 2.811 ± 0.278
1.42IleGln: 1.42 ± 0.2
2.985IleArg: 2.985 ± 0.313
2.319IleSer: 2.319 ± 0.261
3.246IleThr: 3.246 ± 0.298
3.391IleVal: 3.391 ± 0.323
0.812IleTrp: 0.812 ± 0.127
1.304IleTyr: 1.304 ± 0.189
0.0IleXaa: 0.0 ± 0.0
Lys
4.203LysAla: 4.203 ± 0.444
0.667LysCys: 0.667 ± 0.141
1.768LysAsp: 1.768 ± 0.225
2.0LysGlu: 2.0 ± 0.302
1.159LysPhe: 1.159 ± 0.144
3.101LysGly: 3.101 ± 0.376
1.043LysHis: 1.043 ± 0.167
1.797LysIle: 1.797 ± 0.251
2.087LysLys: 2.087 ± 0.281
3.739LysLeu: 3.739 ± 0.337
1.449LysMet: 1.449 ± 0.205
0.725LysAsn: 0.725 ± 0.132
2.609LysPro: 2.609 ± 0.301
1.246LysGln: 1.246 ± 0.187
2.869LysArg: 2.869 ± 0.296
1.797LysSer: 1.797 ± 0.242
2.145LysThr: 2.145 ± 0.252
3.159LysVal: 3.159 ± 0.335
0.985LysTrp: 0.985 ± 0.158
1.391LysTyr: 1.391 ± 0.175
0.0LysXaa: 0.0 ± 0.0
Leu
8.811LeuAla: 8.811 ± 0.576
0.956LeuCys: 0.956 ± 0.165
5.362LeuAsp: 5.362 ± 0.382
5.043LeuGlu: 5.043 ± 0.314
2.377LeuPhe: 2.377 ± 0.275
6.376LeuGly: 6.376 ± 0.463
1.826LeuHis: 1.826 ± 0.217
3.333LeuIle: 3.333 ± 0.333
3.594LeuLys: 3.594 ± 0.326
5.768LeuLeu: 5.768 ± 0.43
1.768LeuMet: 1.768 ± 0.234
3.246LeuAsn: 3.246 ± 0.258
4.55LeuPro: 4.55 ± 0.368
2.724LeuGln: 2.724 ± 0.283
5.478LeuArg: 5.478 ± 0.423
5.159LeuSer: 5.159 ± 0.359
4.898LeuThr: 4.898 ± 0.326
5.101LeuVal: 5.101 ± 0.408
1.246LeuTrp: 1.246 ± 0.171
2.116LeuTyr: 2.116 ± 0.252
0.0LeuXaa: 0.0 ± 0.0
Met
2.406MetAla: 2.406 ± 0.284
0.232MetCys: 0.232 ± 0.099
1.246MetAsp: 1.246 ± 0.189
1.188MetGlu: 1.188 ± 0.195
0.696MetPhe: 0.696 ± 0.14
2.058MetGly: 2.058 ± 0.284
0.522MetHis: 0.522 ± 0.11
1.391MetIle: 1.391 ± 0.21
1.275MetLys: 1.275 ± 0.167
1.478MetLeu: 1.478 ± 0.179
0.609MetMet: 0.609 ± 0.156
1.043MetAsn: 1.043 ± 0.175
1.188MetPro: 1.188 ± 0.195
0.609MetGln: 0.609 ± 0.138
1.507MetArg: 1.507 ± 0.193
2.667MetSer: 2.667 ± 0.261
2.29MetThr: 2.29 ± 0.293
1.13MetVal: 1.13 ± 0.206
0.551MetTrp: 0.551 ± 0.122
0.348MetTyr: 0.348 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
3.101AsnAla: 3.101 ± 0.266
0.348AsnCys: 0.348 ± 0.096
2.261AsnAsp: 2.261 ± 0.261
1.826AsnGlu: 1.826 ± 0.237
1.101AsnPhe: 1.101 ± 0.19
3.971AsnGly: 3.971 ± 0.438
0.841AsnHis: 0.841 ± 0.145
1.594AsnIle: 1.594 ± 0.26
1.275AsnLys: 1.275 ± 0.216
2.551AsnLeu: 2.551 ± 0.289
0.58AsnMet: 0.58 ± 0.122
0.754AsnAsn: 0.754 ± 0.141
2.985AsnPro: 2.985 ± 0.263
0.956AsnGln: 0.956 ± 0.213
2.435AsnArg: 2.435 ± 0.268
1.594AsnSer: 1.594 ± 0.234
1.42AsnThr: 1.42 ± 0.189
2.087AsnVal: 2.087 ± 0.283
0.841AsnTrp: 0.841 ± 0.159
0.812AsnTyr: 0.812 ± 0.171
0.0AsnXaa: 0.0 ± 0.0
Pro
5.014ProAla: 5.014 ± 0.415
0.898ProCys: 0.898 ± 0.168
3.797ProAsp: 3.797 ± 0.33
4.406ProGlu: 4.406 ± 0.31
1.797ProPhe: 1.797 ± 0.237
5.391ProGly: 5.391 ± 0.55
0.841ProHis: 0.841 ± 0.15
2.435ProIle: 2.435 ± 0.281
2.058ProLys: 2.058 ± 0.285
4.029ProLeu: 4.029 ± 0.336
1.101ProMet: 1.101 ± 0.168
2.174ProAsn: 2.174 ± 0.223
3.072ProPro: 3.072 ± 0.308
1.478ProGln: 1.478 ± 0.224
3.13ProArg: 3.13 ± 0.306
2.869ProSer: 2.869 ± 0.343
2.724ProThr: 2.724 ± 0.258
4.435ProVal: 4.435 ± 0.369
1.275ProTrp: 1.275 ± 0.199
1.536ProTyr: 1.536 ± 0.167
0.0ProXaa: 0.0 ± 0.0
Gln
3.536GlnAla: 3.536 ± 0.344
0.435GlnCys: 0.435 ± 0.113
1.391GlnAsp: 1.391 ± 0.203
1.884GlnGlu: 1.884 ± 0.291
1.246GlnPhe: 1.246 ± 0.172
2.203GlnGly: 2.203 ± 0.249
0.667GlnHis: 0.667 ± 0.133
1.768GlnIle: 1.768 ± 0.223
1.739GlnLys: 1.739 ± 0.305
2.29GlnLeu: 2.29 ± 0.292
1.043GlnMet: 1.043 ± 0.179
0.927GlnAsn: 0.927 ± 0.198
2.116GlnPro: 2.116 ± 0.274
1.217GlnGln: 1.217 ± 0.268
2.087GlnArg: 2.087 ± 0.246
1.826GlnSer: 1.826 ± 0.198
1.449GlnThr: 1.449 ± 0.254
2.319GlnVal: 2.319 ± 0.286
0.609GlnTrp: 0.609 ± 0.144
0.667GlnTyr: 0.667 ± 0.145
0.0GlnXaa: 0.0 ± 0.0
Arg
6.521ArgAla: 6.521 ± 0.542
0.985ArgCys: 0.985 ± 0.178
4.55ArgAsp: 4.55 ± 0.362
4.608ArgGlu: 4.608 ± 0.385
2.029ArgPhe: 2.029 ± 0.241
5.159ArgGly: 5.159 ± 0.437
1.507ArgHis: 1.507 ± 0.186
3.507ArgIle: 3.507 ± 0.384
2.985ArgLys: 2.985 ± 0.319
4.377ArgLeu: 4.377 ± 0.399
1.826ArgMet: 1.826 ± 0.229
1.913ArgAsn: 1.913 ± 0.216
3.072ArgPro: 3.072 ± 0.328
2.667ArgGln: 2.667 ± 0.326
5.449ArgArg: 5.449 ± 0.607
3.391ArgSer: 3.391 ± 0.314
3.333ArgThr: 3.333 ± 0.417
5.043ArgVal: 5.043 ± 0.394
2.116ArgTrp: 2.116 ± 0.264
2.348ArgTyr: 2.348 ± 0.279
0.0ArgXaa: 0.0 ± 0.0
Ser
5.391SerAla: 5.391 ± 0.415
0.87SerCys: 0.87 ± 0.157
3.42SerAsp: 3.42 ± 0.355
3.681SerGlu: 3.681 ± 0.366
1.623SerPhe: 1.623 ± 0.295
5.391SerGly: 5.391 ± 0.499
1.159SerHis: 1.159 ± 0.194
2.84SerIle: 2.84 ± 0.274
2.0SerLys: 2.0 ± 0.221
4.637SerLeu: 4.637 ± 0.36
1.449SerMet: 1.449 ± 0.189
1.797SerAsn: 1.797 ± 0.262
2.782SerPro: 2.782 ± 0.248
1.507SerGln: 1.507 ± 0.188
3.362SerArg: 3.362 ± 0.332
3.478SerSer: 3.478 ± 0.351
2.956SerThr: 2.956 ± 0.261
3.536SerVal: 3.536 ± 0.362
1.652SerTrp: 1.652 ± 0.205
1.42SerTyr: 1.42 ± 0.244
0.0SerXaa: 0.0 ± 0.0
Thr
4.811ThrAla: 4.811 ± 0.38
1.043ThrCys: 1.043 ± 0.204
2.898ThrAsp: 2.898 ± 0.299
3.652ThrGlu: 3.652 ± 0.338
2.116ThrPhe: 2.116 ± 0.239
5.101ThrGly: 5.101 ± 0.413
1.014ThrHis: 1.014 ± 0.19
3.101ThrIle: 3.101 ± 0.295
1.942ThrLys: 1.942 ± 0.265
4.985ThrLeu: 4.985 ± 0.352
0.812ThrMet: 0.812 ± 0.164
1.739ThrAsn: 1.739 ± 0.252
4.492ThrPro: 4.492 ± 0.352
1.275ThrGln: 1.275 ± 0.191
3.275ThrArg: 3.275 ± 0.329
2.956ThrSer: 2.956 ± 0.279
2.985ThrThr: 2.985 ± 0.337
4.927ThrVal: 4.927 ± 0.422
1.507ThrTrp: 1.507 ± 0.241
2.058ThrTyr: 2.058 ± 0.267
0.0ThrXaa: 0.0 ± 0.0
Val
6.724ValAla: 6.724 ± 0.465
0.841ValCys: 0.841 ± 0.176
5.797ValAsp: 5.797 ± 0.474
5.507ValGlu: 5.507 ± 0.439
1.826ValPhe: 1.826 ± 0.24
4.579ValGly: 4.579 ± 0.441
1.333ValHis: 1.333 ± 0.227
3.594ValIle: 3.594 ± 0.322
2.58ValLys: 2.58 ± 0.308
4.348ValLeu: 4.348 ± 0.343
1.391ValMet: 1.391 ± 0.2
2.435ValAsn: 2.435 ± 0.245
3.797ValPro: 3.797 ± 0.34
2.493ValGln: 2.493 ± 0.278
4.232ValArg: 4.232 ± 0.393
4.348ValSer: 4.348 ± 0.341
4.608ValThr: 4.608 ± 0.414
5.333ValVal: 5.333 ± 0.49
1.13ValTrp: 1.13 ± 0.214
2.145ValTyr: 2.145 ± 0.239
0.0ValXaa: 0.0 ± 0.0
Trp
1.971TrpAla: 1.971 ± 0.251
0.522TrpCys: 0.522 ± 0.15
1.217TrpAsp: 1.217 ± 0.173
1.159TrpGlu: 1.159 ± 0.197
0.522TrpPhe: 0.522 ± 0.122
1.565TrpGly: 1.565 ± 0.188
0.638TrpHis: 0.638 ± 0.137
1.072TrpIle: 1.072 ± 0.186
0.725TrpLys: 0.725 ± 0.15
2.0TrpLeu: 2.0 ± 0.255
0.58TrpMet: 0.58 ± 0.142
0.87TrpAsn: 0.87 ± 0.175
1.13TrpPro: 1.13 ± 0.195
0.725TrpGln: 0.725 ± 0.147
1.739TrpArg: 1.739 ± 0.257
1.652TrpSer: 1.652 ± 0.217
1.42TrpThr: 1.42 ± 0.234
2.116TrpVal: 2.116 ± 0.236
0.725TrpTrp: 0.725 ± 0.161
0.551TrpTyr: 0.551 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.159TyrAla: 3.159 ± 0.305
0.667TyrCys: 0.667 ± 0.145
2.203TyrAsp: 2.203 ± 0.261
2.058TyrGlu: 2.058 ± 0.236
0.725TyrPhe: 0.725 ± 0.134
3.275TyrGly: 3.275 ± 0.307
0.551TyrHis: 0.551 ± 0.127
1.072TyrIle: 1.072 ± 0.171
1.043TyrLys: 1.043 ± 0.187
2.435TyrLeu: 2.435 ± 0.251
0.232TyrMet: 0.232 ± 0.086
0.841TyrAsn: 0.841 ± 0.172
1.159TyrPro: 1.159 ± 0.194
1.101TyrGln: 1.101 ± 0.156
2.667TyrArg: 2.667 ± 0.332
1.42TyrSer: 1.42 ± 0.181
1.623TyrThr: 1.623 ± 0.197
2.29TyrVal: 2.29 ± 0.306
0.609TyrTrp: 0.609 ± 0.149
1.043TyrTyr: 1.043 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 240 proteins (34503 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski