Dokdonella immobilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Dokdonella

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3897 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4ZZE1|A0A1I4ZZE1_9GAMM Calx-beta domain-containing protein (Fragment) OS=Dokdonella immobilis OX=578942 GN=SAMN05216289_13047 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.0PGSLLVMVVLAGAFSTTALAADD24 pKa = 4.01TKK26 pKa = 10.86IDD28 pKa = 3.65VASANPAVAPVIRR41 pKa = 11.84ASTDD45 pKa = 3.14TVINYY50 pKa = 8.65PGGTPPGNVIGSFDD64 pKa = 3.81AQDD67 pKa = 3.39STFNRR72 pKa = 11.84PVSCATLSGVGTAVPFDD89 pKa = 4.56TITLTNSGASTATVNIRR106 pKa = 11.84VGQQGDD112 pKa = 3.39PSTACGEE119 pKa = 4.37VIDD122 pKa = 4.72PFMVEE127 pKa = 4.22YY128 pKa = 8.69DD129 pKa = 3.56TSFNPASPLTNCLQVNDD146 pKa = 4.36DD147 pKa = 3.88TGGAADD153 pKa = 4.7RR154 pKa = 11.84CPSLTGVSIPSGATRR169 pKa = 11.84VYY171 pKa = 11.2VLTSFDD177 pKa = 3.21NAAVFNYY184 pKa = 9.11EE185 pKa = 3.71VTFAGTTPVTLQSFGIEE202 pKa = 3.88

Molecular weight: 20.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4YNR4|A0A1I4YNR4_9GAMM Signal transduction histidine kinase OS=Dokdonella immobilis OX=578942 GN=SAMN05216289_11879 PE=4 SV=1
MM1 pKa = 6.85ATKK4 pKa = 9.52RR5 pKa = 11.84TYY7 pKa = 10.39QPSNLKK13 pKa = 10.08RR14 pKa = 11.84ARR16 pKa = 11.84THH18 pKa = 6.05GFRR21 pKa = 11.84ARR23 pKa = 11.84MKK25 pKa = 9.02TRR27 pKa = 11.84GGRR30 pKa = 11.84AVINARR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.58GRR41 pKa = 11.84KK42 pKa = 8.75RR43 pKa = 11.84LAVV46 pKa = 3.41

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3897

0

3897

1348227

24

3023

346.0

37.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.482 ± 0.061

0.962 ± 0.015

5.89 ± 0.029

5.545 ± 0.043

3.567 ± 0.024

8.511 ± 0.04

2.2 ± 0.021

4.724 ± 0.024

2.625 ± 0.031

10.589 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.078 ± 0.016

2.714 ± 0.03

5.186 ± 0.027

3.255 ± 0.023

7.757 ± 0.048

5.959 ± 0.036

4.95 ± 0.035

7.239 ± 0.033

1.492 ± 0.02

2.275 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski