Amino acid dipepetide frequency for Sewage-associated circular DNA virus-31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.751AlaAla: 9.751 ± 3.416
0.0AlaCys: 0.0 ± 0.0
1.083AlaAsp: 1.083 ± 1.002
5.417AlaGlu: 5.417 ± 2.418
1.083AlaPhe: 1.083 ± 0.925
5.417AlaGly: 5.417 ± 2.418
1.083AlaHis: 1.083 ± 1.002
2.167AlaIle: 2.167 ± 1.113
2.167AlaLys: 2.167 ± 1.472
4.334AlaLeu: 4.334 ± 2.342
0.0AlaMet: 0.0 ± 0.0
6.501AlaAsn: 6.501 ± 2.095
2.167AlaPro: 2.167 ± 0.691
7.584AlaGln: 7.584 ± 3.79
7.584AlaArg: 7.584 ± 1.369
5.417AlaSer: 5.417 ± 1.983
2.167AlaThr: 2.167 ± 1.113
3.25AlaVal: 3.25 ± 2.208
1.083AlaTrp: 1.083 ± 1.002
5.417AlaTyr: 5.417 ± 2.362
0.0AlaXaa: 0.0 ± 0.0
Cys
2.167CysAla: 2.167 ± 1.472
0.0CysCys: 0.0 ± 0.0
0.0CysAsp: 0.0 ± 0.0
2.167CysGlu: 2.167 ± 0.924
1.083CysPhe: 1.083 ± 0.925
1.083CysGly: 1.083 ± 0.736
0.0CysHis: 0.0 ± 0.0
0.0CysIle: 0.0 ± 0.0
1.083CysLys: 1.083 ± 0.925
1.083CysLeu: 1.083 ± 1.002
1.083CysMet: 1.083 ± 0.736
2.167CysAsn: 2.167 ± 1.472
2.167CysPro: 2.167 ± 1.85
0.0CysGln: 0.0 ± 0.0
0.0CysArg: 0.0 ± 0.0
0.0CysSer: 0.0 ± 0.0
1.083CysThr: 1.083 ± 0.736
3.25CysVal: 3.25 ± 1.337
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
3.25AspAla: 3.25 ± 1.458
1.083AspCys: 1.083 ± 1.002
5.417AspAsp: 5.417 ± 1.132
4.334AspGlu: 4.334 ± 2.944
1.083AspPhe: 1.083 ± 0.736
3.25AspGly: 3.25 ± 0.412
0.0AspHis: 0.0 ± 0.0
2.167AspIle: 2.167 ± 1.472
5.417AspLys: 5.417 ± 1.983
4.334AspLeu: 4.334 ± 0.429
2.167AspMet: 2.167 ± 1.592
2.167AspAsn: 2.167 ± 0.691
2.167AspPro: 2.167 ± 2.004
1.083AspGln: 1.083 ± 0.736
4.334AspArg: 4.334 ± 1.849
1.083AspSer: 1.083 ± 1.002
2.167AspThr: 2.167 ± 1.85
3.25AspVal: 3.25 ± 3.006
1.083AspTrp: 1.083 ± 0.736
1.083AspTyr: 1.083 ± 0.925
0.0AspXaa: 0.0 ± 0.0
Glu
6.501GluAla: 6.501 ± 2.052
1.083GluCys: 1.083 ± 0.736
0.0GluAsp: 0.0 ± 0.0
5.417GluGlu: 5.417 ± 3.68
1.083GluPhe: 1.083 ± 0.736
4.334GluGly: 4.334 ± 1.849
1.083GluHis: 1.083 ± 1.002
2.167GluIle: 2.167 ± 0.924
5.417GluLys: 5.417 ± 2.418
6.501GluLeu: 6.501 ± 2.052
0.0GluMet: 0.0 ± 0.0
1.083GluAsn: 1.083 ± 0.925
3.25GluPro: 3.25 ± 2.208
1.083GluGln: 1.083 ± 0.736
1.083GluArg: 1.083 ± 0.736
5.417GluSer: 5.417 ± 2.628
3.25GluThr: 3.25 ± 1.782
4.334GluVal: 4.334 ± 1.951
2.167GluTrp: 2.167 ± 1.472
7.584GluTyr: 7.584 ± 4.512
0.0GluXaa: 0.0 ± 0.0
Phe
3.25PheAla: 3.25 ± 1.458
0.0PheCys: 0.0 ± 0.0
2.167PheAsp: 2.167 ± 1.85
2.167PheGlu: 2.167 ± 1.472
0.0PhePhe: 0.0 ± 0.0
4.334PheGly: 4.334 ± 0.429
0.0PheHis: 0.0 ± 0.0
2.167PheIle: 2.167 ± 1.113
2.167PheLys: 2.167 ± 0.924
4.334PheLeu: 4.334 ± 4.009
1.083PheMet: 1.083 ± 0.736
1.083PheAsn: 1.083 ± 0.736
0.0PhePro: 0.0 ± 0.0
3.25PheGln: 3.25 ± 1.088
2.167PheArg: 2.167 ± 0.691
0.0PheSer: 0.0 ± 0.0
7.584PheThr: 7.584 ± 2.745
2.167PheVal: 2.167 ± 1.113
1.083PheTrp: 1.083 ± 0.736
0.0PheTyr: 0.0 ± 0.0
0.0PheXaa: 0.0 ± 0.0
Gly
4.334GlyAla: 4.334 ± 3.7
1.083GlyCys: 1.083 ± 0.736
6.501GlyAsp: 6.501 ± 2.674
2.167GlyGlu: 2.167 ± 0.691
1.083GlyPhe: 1.083 ± 0.925
5.417GlyGly: 5.417 ± 2.418
2.167GlyHis: 2.167 ± 0.691
3.25GlyIle: 3.25 ± 2.775
7.584GlyLys: 7.584 ± 1.316
3.25GlyLeu: 3.25 ± 0.412
2.167GlyMet: 2.167 ± 0.691
3.25GlyAsn: 3.25 ± 2.775
4.334GlyPro: 4.334 ± 1.724
5.417GlyGln: 5.417 ± 2.628
5.417GlyArg: 5.417 ± 1.092
5.417GlySer: 5.417 ± 3.494
9.751GlyThr: 9.751 ± 2.739
8.667GlyVal: 8.667 ± 1.994
1.083GlyTrp: 1.083 ± 1.002
4.334GlyTyr: 4.334 ± 2.74
0.0GlyXaa: 0.0 ± 0.0
His
1.083HisAla: 1.083 ± 0.925
0.0HisCys: 0.0 ± 0.0
0.0HisAsp: 0.0 ± 0.0
2.167HisGlu: 2.167 ± 0.924
1.083HisPhe: 1.083 ± 0.736
1.083HisGly: 1.083 ± 0.736
2.167HisHis: 2.167 ± 0.924
0.0HisIle: 0.0 ± 0.0
2.167HisLys: 2.167 ± 2.004
0.0HisLeu: 0.0 ± 0.0
0.0HisMet: 0.0 ± 0.0
1.083HisAsn: 1.083 ± 0.736
1.083HisPro: 1.083 ± 0.736
0.0HisGln: 0.0 ± 0.0
1.083HisArg: 1.083 ± 0.736
1.083HisSer: 1.083 ± 0.925
1.083HisThr: 1.083 ± 0.736
1.083HisVal: 1.083 ± 1.002
1.083HisTrp: 1.083 ± 0.736
1.083HisTyr: 1.083 ± 1.002
0.0HisXaa: 0.0 ± 0.0
Ile
3.25IleAla: 3.25 ± 0.412
0.0IleCys: 0.0 ± 0.0
1.083IleAsp: 1.083 ± 0.736
5.417IleGlu: 5.417 ± 2.178
3.25IlePhe: 3.25 ± 1.782
3.25IleGly: 3.25 ± 0.412
2.167IleHis: 2.167 ± 1.472
3.25IleIle: 3.25 ± 2.208
2.167IleLys: 2.167 ± 0.691
2.167IleLeu: 2.167 ± 0.924
0.0IleMet: 0.0 ± 0.0
2.167IleAsn: 2.167 ± 0.924
0.0IlePro: 0.0 ± 0.0
2.167IleGln: 2.167 ± 0.691
1.083IleArg: 1.083 ± 0.736
3.25IleSer: 3.25 ± 2.775
2.167IleThr: 2.167 ± 1.85
4.334IleVal: 4.334 ± 1.383
0.0IleTrp: 0.0 ± 0.0
3.25IleTyr: 3.25 ± 1.905
0.0IleXaa: 0.0 ± 0.0
Lys
4.334LysAla: 4.334 ± 1.951
1.083LysCys: 1.083 ± 0.736
3.25LysAsp: 3.25 ± 0.412
4.334LysGlu: 4.334 ± 1.951
2.167LysPhe: 2.167 ± 1.113
13.001LysGly: 13.001 ± 1.758
1.083LysHis: 1.083 ± 0.736
1.083LysIle: 1.083 ± 0.736
3.25LysLys: 3.25 ± 2.775
2.167LysLeu: 2.167 ± 0.691
0.0LysMet: 0.0 ± 0.0
1.083LysAsn: 1.083 ± 0.736
1.083LysPro: 1.083 ± 0.736
6.501LysGln: 6.501 ± 0.685
2.167LysArg: 2.167 ± 1.472
1.083LysSer: 1.083 ± 1.002
3.25LysThr: 3.25 ± 2.208
4.334LysVal: 4.334 ± 1.383
4.334LysTrp: 4.334 ± 1.849
8.667LysTyr: 8.667 ± 2.187
0.0LysXaa: 0.0 ± 0.0
Leu
3.25LeuAla: 3.25 ± 1.088
1.083LeuCys: 1.083 ± 0.925
6.501LeuAsp: 6.501 ± 2.315
7.584LeuGlu: 7.584 ± 1.981
2.167LeuPhe: 2.167 ± 2.004
2.167LeuGly: 2.167 ± 0.924
1.083LeuHis: 1.083 ± 1.002
2.167LeuIle: 2.167 ± 0.924
8.667LeuLys: 8.667 ± 2.735
3.25LeuLeu: 3.25 ± 3.006
3.25LeuMet: 3.25 ± 2.751
5.417LeuAsn: 5.417 ± 2.362
1.083LeuPro: 1.083 ± 0.736
2.167LeuGln: 2.167 ± 0.691
4.334LeuArg: 4.334 ± 1.367
3.25LeuSer: 3.25 ± 1.782
3.25LeuThr: 3.25 ± 1.458
2.167LeuVal: 2.167 ± 0.924
0.0LeuTrp: 0.0 ± 0.0
1.083LeuTyr: 1.083 ± 0.736
0.0LeuXaa: 0.0 ± 0.0
Met
2.167MetAla: 2.167 ± 2.004
1.083MetCys: 1.083 ± 0.925
0.0MetAsp: 0.0 ± 0.0
0.0MetGlu: 0.0 ± 0.0
1.083MetPhe: 1.083 ± 0.736
3.25MetGly: 3.25 ± 1.458
0.0MetHis: 0.0 ± 0.0
0.0MetIle: 0.0 ± 0.0
1.083MetLys: 1.083 ± 0.736
0.0MetLeu: 0.0 ± 0.0
2.167MetMet: 2.167 ± 1.472
1.083MetAsn: 1.083 ± 0.925
1.083MetPro: 1.083 ± 1.002
1.083MetGln: 1.083 ± 0.736
1.083MetArg: 1.083 ± 0.736
1.083MetSer: 1.083 ± 1.002
1.083MetThr: 1.083 ± 0.736
0.0MetVal: 0.0 ± 0.0
0.0MetTrp: 0.0 ± 0.0
3.25MetTyr: 3.25 ± 1.088
0.0MetXaa: 0.0 ± 0.0
Asn
1.083AsnAla: 1.083 ± 0.736
0.0AsnCys: 0.0 ± 0.0
3.25AsnAsp: 3.25 ± 1.088
2.167AsnGlu: 2.167 ± 0.924
2.167AsnPhe: 2.167 ± 0.691
7.584AsnGly: 7.584 ± 3.813
0.0AsnHis: 0.0 ± 0.0
1.083AsnIle: 1.083 ± 0.925
3.25AsnLys: 3.25 ± 0.412
4.334AsnLeu: 4.334 ± 2.74
1.083AsnMet: 1.083 ± 0.925
4.334AsnAsn: 4.334 ± 1.093
4.334AsnPro: 4.334 ± 0.429
1.083AsnGln: 1.083 ± 0.925
2.167AsnArg: 2.167 ± 0.924
5.417AsnSer: 5.417 ± 2.086
3.25AsnThr: 3.25 ± 1.784
9.751AsnVal: 9.751 ± 3.416
0.0AsnTrp: 0.0 ± 0.0
1.083AsnTyr: 1.083 ± 1.002
0.0AsnXaa: 0.0 ± 0.0
Pro
1.083ProAla: 1.083 ± 1.002
0.0ProCys: 0.0 ± 0.0
2.167ProAsp: 2.167 ± 2.004
0.0ProGlu: 0.0 ± 0.0
1.083ProPhe: 1.083 ± 0.925
2.167ProGly: 2.167 ± 0.691
1.083ProHis: 1.083 ± 0.736
3.25ProIle: 3.25 ± 1.337
1.083ProLys: 1.083 ± 0.736
1.083ProLeu: 1.083 ± 0.925
0.0ProMet: 0.0 ± 0.0
1.083ProAsn: 1.083 ± 0.736
0.0ProPro: 0.0 ± 0.0
2.167ProGln: 2.167 ± 1.85
2.167ProArg: 2.167 ± 0.691
4.334ProSer: 4.334 ± 1.724
0.0ProThr: 0.0 ± 0.0
2.167ProVal: 2.167 ± 0.691
1.083ProTrp: 1.083 ± 0.736
4.334ProTyr: 4.334 ± 1.849
0.0ProXaa: 0.0 ± 0.0
Gln
2.167GlnAla: 2.167 ± 0.691
1.083GlnCys: 1.083 ± 0.925
2.167GlnAsp: 2.167 ± 1.85
0.0GlnGlu: 0.0 ± 0.0
3.25GlnPhe: 3.25 ± 1.337
3.25GlnGly: 3.25 ± 1.337
0.0GlnHis: 0.0 ± 0.0
4.334GlnIle: 4.334 ± 1.951
1.083GlnLys: 1.083 ± 0.736
4.334GlnLeu: 4.334 ± 1.849
1.083GlnMet: 1.083 ± 0.736
3.25GlnAsn: 3.25 ± 1.458
1.083GlnPro: 1.083 ± 1.002
2.167GlnGln: 2.167 ± 0.691
0.0GlnArg: 0.0 ± 0.0
1.083GlnSer: 1.083 ± 0.925
3.25GlnThr: 3.25 ± 0.412
8.667GlnVal: 8.667 ± 4.684
1.083GlnTrp: 1.083 ± 0.736
1.083GlnTyr: 1.083 ± 0.925
0.0GlnXaa: 0.0 ± 0.0
Arg
4.334ArgAla: 4.334 ± 1.724
2.167ArgCys: 2.167 ± 1.472
0.0ArgAsp: 0.0 ± 0.0
3.25ArgGlu: 3.25 ± 1.782
4.334ArgPhe: 4.334 ± 2.342
3.25ArgGly: 3.25 ± 0.412
2.167ArgHis: 2.167 ± 0.924
2.167ArgIle: 2.167 ± 1.113
2.167ArgLys: 2.167 ± 1.472
6.501ArgLeu: 6.501 ± 1.264
1.083ArgMet: 1.083 ± 1.002
1.083ArgAsn: 1.083 ± 0.925
0.0ArgPro: 0.0 ± 0.0
0.0ArgGln: 0.0 ± 0.0
3.25ArgArg: 3.25 ± 0.412
4.334ArgSer: 4.334 ± 1.849
4.334ArgThr: 4.334 ± 2.342
2.167ArgVal: 2.167 ± 0.924
1.083ArgTrp: 1.083 ± 0.736
3.25ArgTyr: 3.25 ± 1.088
0.0ArgXaa: 0.0 ± 0.0
Ser
5.417SerAla: 5.417 ± 0.539
0.0SerCys: 0.0 ± 0.0
4.334SerAsp: 4.334 ± 1.951
3.25SerGlu: 3.25 ± 1.337
1.083SerPhe: 1.083 ± 0.736
1.083SerGly: 1.083 ± 0.736
0.0SerHis: 0.0 ± 0.0
3.25SerIle: 3.25 ± 1.782
3.25SerLys: 3.25 ± 1.458
2.167SerLeu: 2.167 ± 1.113
0.0SerMet: 0.0 ± 0.0
7.584SerAsn: 7.584 ± 3.813
1.083SerPro: 1.083 ± 0.925
2.167SerGln: 2.167 ± 1.113
2.167SerArg: 2.167 ± 1.85
4.334SerSer: 4.334 ± 2.226
3.25SerThr: 3.25 ± 1.458
3.25SerVal: 3.25 ± 0.412
0.0SerTrp: 0.0 ± 0.0
5.417SerTyr: 5.417 ± 1.508
0.0SerXaa: 0.0 ± 0.0
Thr
6.501ThrAla: 6.501 ± 4.391
2.167ThrCys: 2.167 ± 0.691
4.334ThrAsp: 4.334 ± 2.342
1.083ThrGlu: 1.083 ± 0.736
2.167ThrPhe: 2.167 ± 0.691
7.584ThrGly: 7.584 ± 2.745
2.167ThrHis: 2.167 ± 0.924
1.083ThrIle: 1.083 ± 0.736
8.667ThrLys: 8.667 ± 3.328
4.334ThrLeu: 4.334 ± 0.429
0.0ThrMet: 0.0 ± 0.0
3.25ThrAsn: 3.25 ± 0.412
4.334ThrPro: 4.334 ± 0.429
0.0ThrGln: 0.0 ± 0.0
2.167ThrArg: 2.167 ± 1.113
2.167ThrSer: 2.167 ± 0.691
6.501ThrThr: 6.501 ± 1.452
1.083ThrVal: 1.083 ± 0.925
3.25ThrTrp: 3.25 ± 1.088
3.25ThrTyr: 3.25 ± 1.784
0.0ThrXaa: 0.0 ± 0.0
Val
5.417ValAla: 5.417 ± 1.092
2.167ValCys: 2.167 ± 0.924
5.417ValAsp: 5.417 ± 0.539
6.501ValGlu: 6.501 ± 2.052
2.167ValPhe: 2.167 ± 0.691
8.667ValGly: 8.667 ± 3.298
2.167ValHis: 2.167 ± 0.691
5.417ValIle: 5.417 ± 0.539
4.334ValLys: 4.334 ± 1.383
1.083ValLeu: 1.083 ± 0.736
2.167ValMet: 2.167 ± 0.691
3.25ValAsn: 3.25 ± 0.412
1.083ValPro: 1.083 ± 0.925
2.167ValGln: 2.167 ± 1.113
5.417ValArg: 5.417 ± 1.132
2.167ValSer: 2.167 ± 0.691
4.334ValThr: 4.334 ± 1.383
3.25ValVal: 3.25 ± 1.784
2.167ValTrp: 2.167 ± 1.85
2.167ValTyr: 2.167 ± 1.472
0.0ValXaa: 0.0 ± 0.0
Trp
2.167TrpAla: 2.167 ± 1.85
2.167TrpCys: 2.167 ± 1.472
0.0TrpAsp: 0.0 ± 0.0
4.334TrpGlu: 4.334 ± 2.74
0.0TrpPhe: 0.0 ± 0.0
2.167TrpGly: 2.167 ± 0.691
0.0TrpHis: 0.0 ± 0.0
1.083TrpIle: 1.083 ± 0.736
1.083TrpLys: 1.083 ± 0.736
1.083TrpLeu: 1.083 ± 0.736
0.0TrpMet: 0.0 ± 0.703
3.25TrpAsn: 3.25 ± 2.208
0.0TrpPro: 0.0 ± 0.0
1.083TrpGln: 1.083 ± 0.736
1.083TrpArg: 1.083 ± 0.736
0.0TrpSer: 0.0 ± 0.0
0.0TrpThr: 0.0 ± 0.0
1.083TrpVal: 1.083 ± 1.002
2.167TrpTrp: 2.167 ± 1.472
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.167TyrAla: 2.167 ± 0.924
2.167TyrCys: 2.167 ± 0.924
3.25TyrAsp: 3.25 ± 1.337
1.083TyrGlu: 1.083 ± 1.002
7.584TyrPhe: 7.584 ± 0.406
4.334TyrGly: 4.334 ± 0.429
0.0TyrHis: 0.0 ± 0.0
4.334TyrIle: 4.334 ± 2.342
2.167TyrLys: 2.167 ± 2.004
7.584TyrLeu: 7.584 ± 5.769
2.167TyrMet: 2.167 ± 1.472
3.25TyrAsn: 3.25 ± 1.784
0.0TyrPro: 0.0 ± 0.0
3.25TyrGln: 3.25 ± 3.006
2.167TyrArg: 2.167 ± 0.691
2.167TyrSer: 2.167 ± 1.113
4.334TyrThr: 4.334 ± 1.367
3.25TyrVal: 3.25 ± 1.458
1.083TyrTrp: 1.083 ± 0.736
0.0TyrTyr: 0.0 ± 0.0
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3 proteins (924 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski