Ludwigia leaf distortion betasatellite [India:Amadalavalasa:Hibiscus:2007]
Average proteome isoelectric point is 4.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B2CZA5|B2CZA5_9VIRU C1 protein OS=Ludwigia leaf distortion betasatellite [India:Amadalavalasa:Hibiscus:2007] OX=570832 PE=4 SV=1
MM1 pKa = 7.09 TRR3 pKa = 11.84 SGTNKK8 pKa = 9.11 QGVRR12 pKa = 11.84 FTVDD16 pKa = 2.69 VRR18 pKa = 11.84 LMEE21 pKa = 4.53 DD22 pKa = 3.08 MKK24 pKa = 11.02 IFIHH28 pKa = 5.43 MRR30 pKa = 11.84 IVSTRR35 pKa = 11.84 TPAIIKK41 pKa = 10.2 YY42 pKa = 9.18 EE43 pKa = 4.41 GIVKK47 pKa = 8.12 YY48 pKa = 9.0 TYY50 pKa = 11.57 GDD52 pKa = 3.39 MQVPFDD58 pKa = 4.14 FNGFEE63 pKa = 4.42 GNIIANFLFANNEE76 pKa = 3.96 AKK78 pKa = 10.14 IEE80 pKa = 4.4 EE81 pKa = 4.29 IDD83 pKa = 4.38 IEE85 pKa = 5.4 DD86 pKa = 3.2 IVQRR90 pKa = 11.84 LDD92 pKa = 3.24 ILVLEE97 pKa = 4.49 NPEE100 pKa = 4.0 ILGMDD105 pKa = 3.91 VIEE108 pKa = 4.67 PYY110 pKa = 10.87 VFNKK114 pKa = 10.0 KK115 pKa = 8.02 FTVV118 pKa = 3.26
Molecular weight: 13.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.894
IPC2_protein 4.902
IPC_protein 4.749
Toseland 4.609
ProMoST 4.851
Dawson 4.685
Bjellqvist 4.838
Wikipedia 4.558
Rodwell 4.596
Grimsley 4.52
Solomon 4.685
Lehninger 4.635
Nozaki 4.8
DTASelect 4.94
Thurlkill 4.609
EMBOSS 4.571
Sillero 4.863
Patrickios 4.24
IPC_peptide 4.698
IPC2_peptide 4.851
IPC2.peptide.svr19 4.883
Protein with the highest isoelectric point:
>tr|B2CZA5|B2CZA5_9VIRU C1 protein OS=Ludwigia leaf distortion betasatellite [India:Amadalavalasa:Hibiscus:2007] OX=570832 PE=4 SV=1
MM1 pKa = 7.09 TRR3 pKa = 11.84 SGTNKK8 pKa = 9.11 QGVRR12 pKa = 11.84 FTVDD16 pKa = 2.69 VRR18 pKa = 11.84 LMEE21 pKa = 4.53 DD22 pKa = 3.08 MKK24 pKa = 11.02 IFIHH28 pKa = 5.43 MRR30 pKa = 11.84 IVSTRR35 pKa = 11.84 TPAIIKK41 pKa = 10.2 YY42 pKa = 9.18 EE43 pKa = 4.41 GIVKK47 pKa = 8.12 YY48 pKa = 9.0 TYY50 pKa = 11.57 GDD52 pKa = 3.39 MQVPFDD58 pKa = 4.14 FNGFEE63 pKa = 4.42 GNIIANFLFANNEE76 pKa = 3.96 AKK78 pKa = 10.14 IEE80 pKa = 4.4 EE81 pKa = 4.29 IDD83 pKa = 4.38 IEE85 pKa = 5.4 DD86 pKa = 3.2 IVQRR90 pKa = 11.84 LDD92 pKa = 3.24 ILVLEE97 pKa = 4.49 NPEE100 pKa = 4.0 ILGMDD105 pKa = 3.91 VIEE108 pKa = 4.67 PYY110 pKa = 10.87 VFNKK114 pKa = 10.0 KK115 pKa = 8.02 FTVV118 pKa = 3.26
Molecular weight: 13.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.894
IPC2_protein 4.902
IPC_protein 4.749
Toseland 4.609
ProMoST 4.851
Dawson 4.685
Bjellqvist 4.838
Wikipedia 4.558
Rodwell 4.596
Grimsley 4.52
Solomon 4.685
Lehninger 4.635
Nozaki 4.8
DTASelect 4.94
Thurlkill 4.609
EMBOSS 4.571
Sillero 4.863
Patrickios 4.24
IPC_peptide 4.698
IPC2_peptide 4.851
IPC2.peptide.svr19 4.883
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
118
118
118
118.0
13.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.39 ± 0.0
0.0 ± 0.0
6.78 ± 0.0
8.475 ± 0.0
7.627 ± 0.0
5.932 ± 0.0
0.847 ± 0.0
12.712 ± 0.0
5.932 ± 0.0
5.085 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.085 ± 0.0
6.78 ± 0.0
3.39 ± 0.0
2.542 ± 0.0
5.085 ± 0.0
1.695 ± 0.0
5.932 ± 0.0
9.322 ± 0.0
0.0 ± 0.0
3.39 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here