Suttonella indologenes
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2313 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A380MXZ5|A0A380MXZ5_9GAMM DNA topoisomerase 4 subunit A OS=Suttonella indologenes OX=13276 GN=parC PE=3 SV=1
MM1 pKa = 7.63 SDD3 pKa = 3.53 VEE5 pKa = 4.36 IFDD8 pKa = 4.22 AEE10 pKa = 4.74 VVFTQAAADD19 pKa = 3.86 KK20 pKa = 9.93 VASLMAEE27 pKa = 4.23 EE28 pKa = 4.86 EE29 pKa = 3.89 NDD31 pKa = 3.61 ALKK34 pKa = 10.97 LRR36 pKa = 11.84 VYY38 pKa = 9.07 VTGGGCSGFQYY49 pKa = 10.92 GFMFDD54 pKa = 3.74 EE55 pKa = 5.33 APQEE59 pKa = 4.0 GDD61 pKa = 3.11 AVAVTNGVTLLIDD74 pKa = 4.37 SISYY78 pKa = 9.27 PYY80 pKa = 9.59 LTGAEE85 pKa = 3.5 IDD87 pKa = 3.54 YY88 pKa = 11.26 RR89 pKa = 11.84 EE90 pKa = 4.29 DD91 pKa = 3.08 VKK93 pKa = 11.15 GAQFVIRR100 pKa = 11.84 NPNAQSTCGCGSSFTAA116 pKa = 4.67
Molecular weight: 12.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.897
IPC_protein 3.821
Toseland 3.63
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.541
Solomon 3.783
Lehninger 3.745
Nozaki 3.935
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.948
Patrickios 0.846
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A380N0L5|A0A380N0L5_9GAMM Octaprenyl-diphosphate synthase OS=Suttonella indologenes OX=13276 GN=ispB PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNVSRR13 pKa = 11.84 KK14 pKa = 7.46 RR15 pKa = 11.84 THH17 pKa = 6.15 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATANGRR29 pKa = 11.84 KK30 pKa = 8.91 VLSRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.11 GRR40 pKa = 11.84 HH41 pKa = 5.34 RR42 pKa = 11.84 LTVV45 pKa = 3.07
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2313
0
2313
731157
30
5383
316.1
35.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.618 ± 0.078
0.985 ± 0.021
5.314 ± 0.069
5.951 ± 0.053
3.992 ± 0.044
7.025 ± 0.065
2.403 ± 0.032
6.615 ± 0.047
4.851 ± 0.061
10.506 ± 0.097
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.404 ± 0.037
4.154 ± 0.062
3.907 ± 0.037
5.135 ± 0.058
5.187 ± 0.063
6.025 ± 0.046
4.617 ± 0.069
5.936 ± 0.055
1.207 ± 0.023
3.168 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here