Arsenophonus endosymbiont of Aleurodicus floccissimus
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1780 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3B0J6C6|A0A3B0J6C6_9GAMM Transcriptional regulatory protein OmpR OS=Arsenophonus endosymbiont of Aleurodicus floccissimus OX=2152761 GN=ompR PE=4 SV=1
MM1 pKa = 8.34 DD2 pKa = 3.86 IQTEE6 pKa = 4.24 NEE8 pKa = 4.18 MLWALKK14 pKa = 10.32 KK15 pKa = 10.13 LTVEE19 pKa = 4.39 EE20 pKa = 4.85 EE21 pKa = 4.96 GFCQPGGEE29 pKa = 4.17 YY30 pKa = 10.2 LYY32 pKa = 11.02 EE33 pKa = 4.42 SLTNAYY39 pKa = 7.58 LTQKK43 pKa = 10.95 LSDD46 pKa = 3.49 TDD48 pKa = 3.86 KK49 pKa = 10.98 SDD51 pKa = 4.42 EE52 pKa = 4.19 YY53 pKa = 10.93 EE54 pKa = 3.91 AWLLALEE61 pKa = 4.45 TTDD64 pKa = 5.02 GFDD67 pKa = 3.08 EE68 pKa = 4.2 VLYY71 pKa = 10.95 DD72 pKa = 3.31 VTQKK76 pKa = 10.43 VEE78 pKa = 4.01 QILYY82 pKa = 10.86 LMQCRR87 pKa = 11.84 DD88 pKa = 3.23 AYY90 pKa = 11.3 YY91 pKa = 10.04 EE92 pKa = 4.32 VPAA95 pKa = 5.67
Molecular weight: 11.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.831
IPC2_protein 3.872
IPC_protein 3.783
Toseland 3.605
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.63
Grimsley 3.528
Solomon 3.732
Lehninger 3.694
Nozaki 3.884
DTASelect 4.012
Thurlkill 3.656
EMBOSS 3.668
Sillero 3.897
Patrickios 0.362
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A3B0IXE6|A0A3B0IXE6_9GAMM Transcription termination factor Rho OS=Arsenophonus endosymbiont of Aleurodicus floccissimus OX=2152761 GN=rho PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSSKK47 pKa = 11.16
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1780
0
1780
407451
29
1664
228.9
25.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.83 ± 0.064
1.173 ± 0.022
5.061 ± 0.041
5.752 ± 0.065
3.991 ± 0.043
6.357 ± 0.057
2.27 ± 0.027
8.056 ± 0.057
5.992 ± 0.048
10.493 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.672 ± 0.032
4.876 ± 0.041
3.88 ± 0.038
4.622 ± 0.052
4.914 ± 0.057
6.004 ± 0.043
5.363 ± 0.04
6.179 ± 0.057
1.222 ± 0.026
3.293 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here