Halorubrum pleomorphic virus 10
Average proteome isoelectric point is 5.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A410N6N8|A0A410N6N8_9VIRU ORF9 OS=Halorubrum pleomorphic virus 10 OX=2507576 GN=HRPV10-gp11 PE=4 SV=1
MM1 pKa = 7.78 AKK3 pKa = 8.66 MQMDD7 pKa = 5.64 AIDD10 pKa = 3.91 ASLFVLNTMAGFLLVGIGTFEE31 pKa = 4.34 LFGVDD36 pKa = 4.79 FGATLFSPAGIGLSTAWVIGYY57 pKa = 9.33 AAVVGTLVTNDD68 pKa = 3.22 NAEE71 pKa = 4.14 LSSLTNDD78 pKa = 3.35 VQDD81 pKa = 4.16 LEE83 pKa = 4.52 GYY85 pKa = 10.03 YY86 pKa = 10.21 YY87 pKa = 11.12 AAALGTLLLPVAFVVFPDD105 pKa = 3.87 TVGSFFKK112 pKa = 11.03 SADD115 pKa = 3.44 LWGLAYY121 pKa = 10.33 VVLVTSGQAALGWMLL136 pKa = 4.07
Molecular weight: 14.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.961
IPC2_protein 3.478
IPC_protein 3.389
Toseland 3.198
ProMoST 3.63
Dawson 3.427
Bjellqvist 3.605
Wikipedia 3.439
Rodwell 3.249
Grimsley 3.121
Solomon 3.363
Lehninger 3.312
Nozaki 3.579
DTASelect 3.795
Thurlkill 3.3
EMBOSS 3.439
Sillero 3.528
Patrickios 0.172
IPC_peptide 3.35
IPC2_peptide 3.49
IPC2.peptide.svr19 3.649
Protein with the highest isoelectric point:
>tr|A0A410N6Y6|A0A410N6Y6_9VIRU ORF5 OS=Halorubrum pleomorphic virus 10 OX=2507576 GN=HRPV10-gp05 PE=4 SV=1
MM1 pKa = 7.75 AKK3 pKa = 8.31 QTARR7 pKa = 11.84 DD8 pKa = 3.92 RR9 pKa = 11.84 CWNRR13 pKa = 11.84 ALDD16 pKa = 3.66 IVLKK20 pKa = 10.65 KK21 pKa = 10.79 GGVVRR26 pKa = 11.84 KK27 pKa = 9.47 QDD29 pKa = 4.13 LILAEE34 pKa = 4.3 EE35 pKa = 4.35 VSEE38 pKa = 4.2 RR39 pKa = 11.84 TAADD43 pKa = 3.5 VLSTMTDD50 pKa = 3.22 MGWLKK55 pKa = 10.57 RR56 pKa = 11.84 EE57 pKa = 4.05 YY58 pKa = 11.02 VPGPKK63 pKa = 9.43 ADD65 pKa = 2.77 NWRR68 pKa = 11.84 RR69 pKa = 11.84 GDD71 pKa = 3.87 EE72 pKa = 4.19 LPATLSTEE80 pKa = 4.13 GAKK83 pKa = 10.33
Molecular weight: 9.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.277
IPC2_protein 8.024
IPC_protein 8.287
Toseland 8.931
ProMoST 8.99
Dawson 9.004
Bjellqvist 8.887
Wikipedia 9.033
Rodwell 9.18
Grimsley 8.639
Solomon 9.399
Lehninger 9.414
Nozaki 9.18
DTASelect 8.799
Thurlkill 8.96
EMBOSS 9.268
Sillero 9.151
Patrickios 7.673
IPC_peptide 9.399
IPC2_peptide 7.644
IPC2.peptide.svr19 7.649
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
2711
46
538
208.5
22.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.918 ± 1.067
0.59 ± 0.289
7.709 ± 0.641
7.894 ± 0.873
2.619 ± 0.388
8.521 ± 0.806
1.66 ± 0.606
4.869 ± 0.397
3.467 ± 0.418
7.488 ± 0.732
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.176 ± 0.243
3.652 ± 0.332
3.726 ± 0.32
2.656 ± 0.256
5.681 ± 0.751
6.898 ± 0.404
6.824 ± 0.613
7.451 ± 0.947
1.475 ± 0.241
3.726 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here