Pseudomonas phage Lana

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lanavirus; Pseudomonas virus Lana

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W6C1|A0A481W6C1_9CAUD Toxin-antitoxin protein OS=Pseudomonas phage Lana OX=2530172 PE=4 SV=1
MM1 pKa = 7.68AIIDD5 pKa = 4.14RR6 pKa = 11.84IEE8 pKa = 3.96YY9 pKa = 9.61SVCEE13 pKa = 3.98DD14 pKa = 3.8CFTVVAHH21 pKa = 6.12GHH23 pKa = 5.8SDD25 pKa = 3.34HH26 pKa = 6.77TSEE29 pKa = 5.99ADD31 pKa = 4.09DD32 pKa = 3.81DD33 pKa = 4.46HH34 pKa = 8.56LEE36 pKa = 3.68QRR38 pKa = 11.84MISEE42 pKa = 4.19RR43 pKa = 11.84GDD45 pKa = 3.15RR46 pKa = 11.84DD47 pKa = 4.22GYY49 pKa = 7.86WTTGVEE55 pKa = 3.99PTEE58 pKa = 4.77DD59 pKa = 3.62DD60 pKa = 3.94EE61 pKa = 4.92EE62 pKa = 6.03GNGYY66 pKa = 10.43DD67 pKa = 3.95EE68 pKa = 6.37FSSCDD73 pKa = 3.6CEE75 pKa = 4.39LCNDD79 pKa = 3.8GLAGSRR85 pKa = 11.84HH86 pKa = 5.11GVSLILTKK94 pKa = 10.76EE95 pKa = 4.03EE96 pKa = 3.94EE97 pKa = 4.16

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W5Y4|A0A481W5Y4_9CAUD Uncharacterized protein OS=Pseudomonas phage Lana OX=2530172 PE=4 SV=1
MM1 pKa = 7.77RR2 pKa = 11.84FFVDD6 pKa = 3.31HH7 pKa = 5.61QRR9 pKa = 11.84RR10 pKa = 11.84LVRR13 pKa = 11.84TIGATDD19 pKa = 3.65PTPPPRR25 pKa = 11.84WVEE28 pKa = 4.22VTATEE33 pKa = 3.9YY34 pKa = 11.23DD35 pKa = 3.4QFRR38 pKa = 11.84KK39 pKa = 8.31EE40 pKa = 4.06TASYY44 pKa = 9.5SAKK47 pKa = 10.51ALRR50 pKa = 11.84LLRR53 pKa = 11.84AQAA56 pKa = 3.6

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

132

0

132

27690

38

2505

209.8

23.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.213 ± 0.419

1.112 ± 0.135

6.219 ± 0.216

6.591 ± 0.346

3.398 ± 0.156

7.169 ± 0.187

2.189 ± 0.196

4.763 ± 0.181

5.684 ± 0.314

8.815 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.802 ± 0.136

3.705 ± 0.18

4.218 ± 0.249

4.052 ± 0.142

5.218 ± 0.197

5.749 ± 0.345

6.638 ± 0.434

7.064 ± 0.222

1.546 ± 0.11

2.853 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski