Thiopseudomonas denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Thiopseudomonas

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2567 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6TVL4|A0A4R6TVL4_9GAMM Cyclic pyranopterin monophosphate synthase OS=Thiopseudomonas denitrificans OX=1501432 GN=moaC PE=3 SV=1
MM1 pKa = 7.15SQPTSAYY8 pKa = 8.04MAFMQLLTSQGQHH21 pKa = 6.06FSPAEE26 pKa = 3.71LHH28 pKa = 6.28GLLVGHH34 pKa = 6.41SCAGAGFAPQAWLDD48 pKa = 3.58NAAQLFDD55 pKa = 3.85GDD57 pKa = 4.54IPASLQPALTGLQDD71 pKa = 3.49MLKK74 pKa = 9.12TEE76 pKa = 4.59ISADD80 pKa = 3.34DD81 pKa = 3.88CMAITLLLPGDD92 pKa = 4.82DD93 pKa = 4.83DD94 pKa = 4.4SLPARR99 pKa = 11.84SQALGQWCQGFLSGFGSSIGDD120 pKa = 3.14RR121 pKa = 11.84KK122 pKa = 10.53LSAEE126 pKa = 3.78ARR128 pKa = 11.84EE129 pKa = 3.91ILEE132 pKa = 4.96DD133 pKa = 3.85YY134 pKa = 10.82VAVAQISSGDD144 pKa = 3.64DD145 pKa = 3.33EE146 pKa = 5.12EE147 pKa = 5.34EE148 pKa = 4.5GGEE151 pKa = 4.17AADD154 pKa = 3.5EE155 pKa = 4.25ASFMEE160 pKa = 4.15VSEE163 pKa = 4.45YY164 pKa = 11.21LRR166 pKa = 11.84MTAVFLFTEE175 pKa = 4.86CNTPAPTPDD184 pKa = 3.53SQPEE188 pKa = 3.86QPAIHH193 pKa = 6.6

Molecular weight: 20.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6TX45|A0A4R6TX45_9GAMM Murein L D-transpeptidase YcbB/YkuD OS=Thiopseudomonas denitrificans OX=1501432 GN=DFQ45_10421 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 9.2RR14 pKa = 11.84DD15 pKa = 3.06HH16 pKa = 6.58GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27GGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2567

0

2567

833170

29

2551

324.6

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.452 ± 0.058

0.997 ± 0.017

5.38 ± 0.034

5.995 ± 0.054

3.546 ± 0.033

7.676 ± 0.042

2.345 ± 0.024

4.971 ± 0.034

3.399 ± 0.037

11.981 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.022

3.175 ± 0.032

4.535 ± 0.038

5.342 ± 0.057

6.418 ± 0.041

5.718 ± 0.039

4.722 ± 0.037

6.905 ± 0.044

1.392 ± 0.022

2.538 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski