Microbacterium phage Warren

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Appavirus; unclassified Appavirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8N6W6|A0A4P8N6W6_9CAUD Uncharacterized protein OS=Microbacterium phage Warren OX=2565528 GN=43 PE=4 SV=1
MM1 pKa = 7.18TKK3 pKa = 10.04RR4 pKa = 11.84RR5 pKa = 11.84STIMTEE11 pKa = 3.82QTVNPLPAFNIDD23 pKa = 3.96DD24 pKa = 3.82VDD26 pKa = 4.51DD27 pKa = 4.96GEE29 pKa = 4.94RR30 pKa = 11.84NWAALALCVVDD41 pKa = 4.83IEE43 pKa = 4.46TRR45 pKa = 11.84RR46 pKa = 11.84CIYY49 pKa = 10.26CGSAAIWDD57 pKa = 4.23TYY59 pKa = 11.23AEE61 pKa = 4.49YY62 pKa = 10.67VAHH65 pKa = 7.0FYY67 pKa = 11.19AFEE70 pKa = 4.21YY71 pKa = 10.88EE72 pKa = 4.11GDD74 pKa = 3.56SWHH77 pKa = 7.19APDD80 pKa = 5.99DD81 pKa = 3.77EE82 pKa = 6.65ADD84 pKa = 3.59FDD86 pKa = 4.51RR87 pKa = 11.84VSSLCLATADD97 pKa = 4.3EE98 pKa = 4.64NADD101 pKa = 3.38DD102 pKa = 4.2SAVVRR107 pKa = 4.6

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1EYR3|A0A4V1EYR3_9CAUD Uncharacterized protein OS=Microbacterium phage Warren OX=2565528 GN=63 PE=4 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84RR3 pKa = 11.84DD4 pKa = 3.05KK5 pKa = 11.26SFEE8 pKa = 3.61FGRR11 pKa = 11.84WVGRR15 pKa = 11.84YY16 pKa = 8.16IVGPLLVLVIIGALVGLIALLVVGIGNTLGAA47 pKa = 4.62

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12499

42

732

195.3

21.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.633 ± 0.47

0.536 ± 0.114

6.873 ± 0.251

6.641 ± 0.291

2.584 ± 0.218

8.457 ± 0.341

1.792 ± 0.202

4.744 ± 0.229

3.048 ± 0.266

7.561 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.173

2.448 ± 0.178

5.064 ± 0.338

3.152 ± 0.171

7.721 ± 0.401

5.784 ± 0.266

6.641 ± 0.291

7.673 ± 0.248

1.896 ± 0.167

2.256 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski