Bat coronavirus HKU9 (BtCoV) (BtCoV/HKU9)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Nobecovirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A3EXG9|VME1_BCHK9 Membrane protein OS=Bat coronavirus HKU9 OX=694006 GN=M PE=3 SV=1
MM1 pKa = 7.31YY2 pKa = 10.07DD3 pKa = 3.06IVGTNNSILIANVLVLIIICLLVVIVGCALLLILQFVFGVCGFVFKK49 pKa = 9.25FVCKK53 pKa = 8.8PTILVYY59 pKa = 11.02NKK61 pKa = 9.42FRR63 pKa = 11.84NEE65 pKa = 3.56SLLNEE70 pKa = 4.19RR71 pKa = 11.84EE72 pKa = 4.03EE73 pKa = 4.17LLCDD77 pKa = 3.64NVV79 pKa = 3.42

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A3EXH1|NS7A_BCHK9 Non-structural protein 7a OS=Bat coronavirus HKU9 OX=694006 GN=7a PE=3 SV=1
MM1 pKa = 7.53SGRR4 pKa = 11.84NRR6 pKa = 11.84SRR8 pKa = 11.84SGTPSPKK15 pKa = 9.14VTFKK19 pKa = 11.02QEE21 pKa = 3.89SDD23 pKa = 3.52GSDD26 pKa = 3.37SEE28 pKa = 4.52SEE30 pKa = 3.92RR31 pKa = 11.84RR32 pKa = 11.84NGNRR36 pKa = 11.84NGARR40 pKa = 11.84PKK42 pKa = 10.5NNNSRR47 pKa = 11.84GSAPKK52 pKa = 9.89PEE54 pKa = 4.43KK55 pKa = 10.23PKK57 pKa = 10.56AAPPQNVSWFAPLVQTGKK75 pKa = 10.94AEE77 pKa = 3.93LRR79 pKa = 11.84FPRR82 pKa = 11.84GEE84 pKa = 4.09GVPVSQGVDD93 pKa = 2.91STYY96 pKa = 11.3EE97 pKa = 3.4HH98 pKa = 7.18GYY100 pKa = 8.24WLRR103 pKa = 11.84TQRR106 pKa = 11.84SFQKK110 pKa = 10.63GGKK113 pKa = 8.51QVLANPRR120 pKa = 11.84WYY122 pKa = 10.14FYY124 pKa = 10.09YY125 pKa = 10.29TGTGRR130 pKa = 11.84FGDD133 pKa = 3.82LRR135 pKa = 11.84FGTKK139 pKa = 10.25NPDD142 pKa = 3.36IVWVGQEE149 pKa = 3.73GANINRR155 pKa = 11.84LGDD158 pKa = 3.28MGTRR162 pKa = 11.84NPSNDD167 pKa = 2.83GAIPVQLAGGIPKK180 pKa = 10.3GFYY183 pKa = 10.56AEE185 pKa = 4.4GRR187 pKa = 11.84GSRR190 pKa = 11.84GNSRR194 pKa = 11.84SSSRR198 pKa = 11.84NSSRR202 pKa = 11.84ASSRR206 pKa = 11.84GNSRR210 pKa = 11.84ASSRR214 pKa = 11.84GASPGRR220 pKa = 11.84PAANPSTEE228 pKa = 3.28PWMAYY233 pKa = 9.55LVQKK237 pKa = 10.22LEE239 pKa = 4.11RR240 pKa = 11.84LEE242 pKa = 4.27SQVSGTKK249 pKa = 9.77PATKK253 pKa = 10.5NPVQVTKK260 pKa = 11.33NEE262 pKa = 3.71AAANAKK268 pKa = 9.82KK269 pKa = 10.2LRR271 pKa = 11.84HH272 pKa = 6.07KK273 pKa = 9.87RR274 pKa = 11.84TAHH277 pKa = 6.37KK278 pKa = 10.71GSGVTVNYY286 pKa = 9.67GRR288 pKa = 11.84RR289 pKa = 11.84GPGDD293 pKa = 3.4LEE295 pKa = 4.89GNFGDD300 pKa = 4.8RR301 pKa = 11.84EE302 pKa = 4.21MIKK305 pKa = 10.58LGTDD309 pKa = 3.39DD310 pKa = 4.27PRR312 pKa = 11.84FAAAAQMAPNVSSFLFMSHH331 pKa = 7.13LSTRR335 pKa = 11.84DD336 pKa = 3.01EE337 pKa = 5.85DD338 pKa = 3.98DD339 pKa = 5.0ALWLHH344 pKa = 5.98YY345 pKa = 10.2KK346 pKa = 10.54GAIKK350 pKa = 10.4LPKK353 pKa = 9.74DD354 pKa = 3.61DD355 pKa = 5.0PNYY358 pKa = 9.53EE359 pKa = 3.75QWTKK363 pKa = 10.82ILAEE367 pKa = 4.18NLNAYY372 pKa = 9.73KK373 pKa = 10.34DD374 pKa = 4.19FPPTEE379 pKa = 4.01PKK381 pKa = 9.85KK382 pKa = 10.54DD383 pKa = 3.37KK384 pKa = 10.64KK385 pKa = 10.79KK386 pKa = 10.82KK387 pKa = 10.43EE388 pKa = 3.69EE389 pKa = 3.97TAQDD393 pKa = 3.47TVIFEE398 pKa = 4.52DD399 pKa = 4.13ASTGTDD405 pKa = 3.18QTVVKK410 pKa = 10.27VWVKK414 pKa = 10.92DD415 pKa = 3.4QDD417 pKa = 3.61AQTDD421 pKa = 4.02DD422 pKa = 3.18EE423 pKa = 4.56WLGGDD428 pKa = 3.28EE429 pKa = 4.37TVYY432 pKa = 10.85EE433 pKa = 4.85DD434 pKa = 4.34EE435 pKa = 5.21DD436 pKa = 4.66DD437 pKa = 3.79RR438 pKa = 11.84PKK440 pKa = 9.49TQRR443 pKa = 11.84RR444 pKa = 11.84HH445 pKa = 5.22KK446 pKa = 10.5KK447 pKa = 9.72RR448 pKa = 11.84GSTASRR454 pKa = 11.84VTIADD459 pKa = 3.46PTNAGAEE466 pKa = 4.13RR467 pKa = 11.84SS468 pKa = 3.74

Molecular weight: 51.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

13775

79

6930

1530.6

169.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.535 ± 0.145

3.114 ± 0.289

5.314 ± 0.183

3.652 ± 0.239

4.436 ± 0.25

6.475 ± 0.252

1.808 ± 0.133

4.414 ± 0.34

4.53 ± 0.371

9.829 ± 0.441

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.133

4.82 ± 0.586

4.668 ± 0.258

3.071 ± 0.167

4.247 ± 0.463

6.904 ± 0.398

6.628 ± 0.169

9.851 ± 0.624

1.299 ± 0.109

4.9 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski