Bacillus thermoamylovorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Caldibacillus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3440 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090KSB9|A0A090KSB9_9BACI Uncharacterized protein OS=Bacillus thermoamylovorans OX=35841 GN=BT1A1_1783 PE=4 SV=1
MM1 pKa = 7.74SNLEE5 pKa = 3.82WLSRR9 pKa = 11.84IEE11 pKa = 3.89QAISISLPEE20 pKa = 4.08VSAKK24 pKa = 10.23FDD26 pKa = 3.87DD27 pKa = 4.04YY28 pKa = 11.4EE29 pKa = 4.59IRR31 pKa = 11.84LTVNTTKK38 pKa = 10.51KK39 pKa = 10.0HH40 pKa = 5.88PSLSFYY46 pKa = 10.55TEE48 pKa = 3.49IDD50 pKa = 3.21TKK52 pKa = 10.77IFEE55 pKa = 4.26FCTIYY60 pKa = 10.54FDD62 pKa = 4.51PVNQEE67 pKa = 3.72LYY69 pKa = 10.15SYY71 pKa = 10.14YY72 pKa = 9.81WNEE75 pKa = 3.84DD76 pKa = 2.99FEE78 pKa = 5.73LNSKK82 pKa = 9.78ILFTEE87 pKa = 4.14LEE89 pKa = 4.19EE90 pKa = 5.99IIDD93 pKa = 4.27FIYY96 pKa = 10.93DD97 pKa = 3.4AFFDD101 pKa = 4.13FLDD104 pKa = 3.95HH105 pKa = 8.47VEE107 pKa = 4.37EE108 pKa = 5.77DD109 pKa = 4.15DD110 pKa = 4.5EE111 pKa = 4.73NEE113 pKa = 4.13QVEE116 pKa = 4.66SSS118 pKa = 3.36

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090J2M8|A0A090J2M8_9BACI Uncharacterized protein OS=Bacillus thermoamylovorans OX=35841 GN=B4064_1454 PE=4 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84RR3 pKa = 11.84KK4 pKa = 7.22THH6 pKa = 6.48FFGDD10 pKa = 4.35EE11 pKa = 3.7ITSRR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 3.75FWAGNSIFWRR27 pKa = 11.84RR28 pKa = 11.84NSFSSSFLGWKK39 pKa = 10.12CYY41 pKa = 10.28FLASKK46 pKa = 10.7LLLIVFLGPEE56 pKa = 4.05TPLFGDD62 pKa = 3.38EE63 pKa = 4.32TLSCRR68 pKa = 11.84HH69 pKa = 5.77FEE71 pKa = 4.52AEE73 pKa = 4.51NSLFWRR79 pKa = 11.84RR80 pKa = 11.84DD81 pKa = 3.35HH82 pKa = 7.17FSSSLLGGKK91 pKa = 8.42LHH93 pKa = 6.93FLATILVLVVIFRR106 pKa = 11.84LEE108 pKa = 3.63MLFFGDD114 pKa = 4.47EE115 pKa = 3.93ITSRR119 pKa = 11.84RR120 pKa = 11.84HH121 pKa = 4.32FWPGNSIFWRR131 pKa = 11.84RR132 pKa = 11.84NPFSSSFWGRR142 pKa = 11.84KK143 pKa = 6.93LHH145 pKa = 6.96FLATKK150 pKa = 10.2LVLVVIFVRR159 pKa = 11.84KK160 pKa = 9.98SPFFGDD166 pKa = 3.18EE167 pKa = 4.05TLSRR171 pKa = 11.84RR172 pKa = 11.84HH173 pKa = 5.9FGAGNAFFWRR183 pKa = 11.84RR184 pKa = 11.84NSFSSSFLCGNLHH197 pKa = 6.96FLATRR202 pKa = 11.84LILVVVFGRR211 pKa = 11.84KK212 pKa = 6.17THH214 pKa = 6.63FFGDD218 pKa = 3.7DD219 pKa = 3.0TLSRR223 pKa = 11.84RR224 pKa = 11.84RR225 pKa = 11.84FWTEE229 pKa = 2.88NSLFWRR235 pKa = 11.84RR236 pKa = 11.84DD237 pKa = 3.53PFSSPFWDD245 pKa = 3.3RR246 pKa = 11.84KK247 pKa = 10.36RR248 pKa = 11.84YY249 pKa = 9.25FLATTLILVAFFAPEE264 pKa = 3.76PPLFGDD270 pKa = 3.72EE271 pKa = 4.42TFSSSFLCVNKK282 pKa = 9.07YY283 pKa = 8.3TNSRR287 pKa = 11.84FSAFDD292 pKa = 3.47CLL294 pKa = 4.38

Molecular weight: 34.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3440

0

3440

983123

29

2122

285.8

32.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.531 ± 0.042

0.703 ± 0.012

5.029 ± 0.036

7.475 ± 0.05

4.791 ± 0.036

6.73 ± 0.038

2.016 ± 0.019

8.563 ± 0.043

7.41 ± 0.04

9.685 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.02

4.773 ± 0.033

3.589 ± 0.02

3.713 ± 0.029

3.97 ± 0.034

5.566 ± 0.032

5.363 ± 0.026

6.844 ± 0.035

0.98 ± 0.018

3.613 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski