Ranid herpesvirus 2
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 147 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q14W02|Q14W02_9VIRU ORF104 OS=Ranid herpesvirus 2 OX=389214 PE=4 SV=1
MM1 pKa = 7.09 FVKK4 pKa = 9.61 LTVVAIALACAVQRR18 pKa = 11.84 HH19 pKa = 5.4 AALVPSSNDD28 pKa = 3.02 PEE30 pKa = 4.58 KK31 pKa = 11.38 YY32 pKa = 9.47 IDD34 pKa = 3.64 EE35 pKa = 5.1 DD36 pKa = 4.94 GYY38 pKa = 11.31 GPTMLTKK45 pKa = 10.09 FQIPQVPSFRR55 pKa = 11.84 GTASPTEE62 pKa = 4.18 DD63 pKa = 3.47 EE64 pKa = 4.38 EE65 pKa = 4.91 EE66 pKa = 3.99 EE67 pKa = 4.57 VVVVEE72 pKa = 4.59 RR73 pKa = 11.84 VPPSAAAQEE82 pKa = 4.38 EE83 pKa = 4.78 EE84 pKa = 4.81 TVAEE88 pKa = 4.35 AATAEE93 pKa = 4.53 TPTEE97 pKa = 4.33 TVDD100 pKa = 3.46 EE101 pKa = 4.95 APTEE105 pKa = 4.12 EE106 pKa = 4.49 TPAEE110 pKa = 4.2 AEE112 pKa = 4.14 PTVLEE117 pKa = 4.43 VPVDD121 pKa = 3.72 TAEE124 pKa = 4.18 TTTPAGVEE132 pKa = 4.2 EE133 pKa = 4.31 EE134 pKa = 5.03 SITTTTPSPEE144 pKa = 3.97 EE145 pKa = 4.06 VEE147 pKa = 4.22 RR148 pKa = 11.84 TTLGVMLGLSILTTTGTTLSLVVEE172 pKa = 4.79 EE173 pKa = 4.78 PTNTTATPTTTTKK186 pKa = 10.78 AVNTTPEE193 pKa = 4.23 DD194 pKa = 3.79 YY195 pKa = 10.62 KK196 pKa = 11.51 DD197 pKa = 3.59 EE198 pKa = 5.42 DD199 pKa = 3.75 FDD201 pKa = 4.42 EE202 pKa = 5.13 YY203 pKa = 11.17 EE204 pKa = 4.22 SSDD207 pKa = 3.83 TEE209 pKa = 4.02 LHH211 pKa = 6.96 DD212 pKa = 4.57 RR213 pKa = 11.84 LPTIGGYY220 pKa = 5.84 TTPPRR225 pKa = 11.84 STTASNTTVNGTSSKK240 pKa = 10.43 PDD242 pKa = 3.24 QKK244 pKa = 10.97 PNQSNGSGPGSDD256 pKa = 3.19 FGVYY260 pKa = 7.32 VHH262 pKa = 6.96 IFSKK266 pKa = 10.04 ATAEE270 pKa = 5.51 RR271 pKa = 11.84 GPTWQAALLISVVFIMGVVFF291 pKa = 5.1
Molecular weight: 30.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.263
IPC2_protein 4.075
IPC_protein 4.012
Toseland 3.846
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.795
Rodwell 3.846
Grimsley 3.757
Solomon 3.948
Lehninger 3.897
Nozaki 4.05
DTASelect 4.164
Thurlkill 3.846
EMBOSS 3.821
Sillero 4.113
Patrickios 3.541
IPC_peptide 3.948
IPC2_peptide 4.101
IPC2.peptide.svr19 4.032
Protein with the highest isoelectric point:
>tr|Q14VX4|Q14VX4_9VIRU ORF132 OS=Ranid herpesvirus 2 OX=389214 PE=4 SV=1
MM1 pKa = 7.61 RR2 pKa = 11.84 VLVLAAFLWAATGFNPRR19 pKa = 11.84 VARR22 pKa = 11.84 SADD25 pKa = 3.43 FPLNDD30 pKa = 3.47 QEE32 pKa = 4.22 FARR35 pKa = 11.84 VKK37 pKa = 10.55 EE38 pKa = 3.83 LLNRR42 pKa = 11.84 NSVRR46 pKa = 11.84 YY47 pKa = 9.51 KK48 pKa = 10.17 RR49 pKa = 11.84 TDD51 pKa = 3.18 GPPRR55 pKa = 11.84 DD56 pKa = 3.73 VDD58 pKa = 3.28 ATFYY62 pKa = 10.91 SVRR65 pKa = 11.84 PPPAYY70 pKa = 8.7 TAAFKK75 pKa = 10.53 KK76 pKa = 10.12 IYY78 pKa = 8.81 TSRR81 pKa = 11.84 SVRR84 pKa = 11.84 SFCLRR89 pKa = 11.84 DD90 pKa = 3.43 YY91 pKa = 11.63 VKK93 pKa = 10.98 GKK95 pKa = 7.66 TRR97 pKa = 11.84 SVEE100 pKa = 3.71 ASSCIFGLKK109 pKa = 9.63 KK110 pKa = 10.33 QLFLLRR116 pKa = 11.84 STRR119 pKa = 11.84 VQNITSEE126 pKa = 4.41 PPCLEE131 pKa = 3.85 VRR133 pKa = 11.84 PVTSWGYY140 pKa = 10.53 IYY142 pKa = 11.28 VNALNQDD149 pKa = 3.74 RR150 pKa = 11.84 IKK152 pKa = 11.1 INMGKK157 pKa = 9.29 KK158 pKa = 9.55 LQMMPCKK165 pKa = 10.0 PRR167 pKa = 4.4
Molecular weight: 19.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.692
IPC_protein 10.233
Toseland 10.496
ProMoST 10.248
Dawson 10.628
Bjellqvist 10.321
Wikipedia 10.804
Rodwell 10.965
Grimsley 10.687
Solomon 10.687
Lehninger 10.657
Nozaki 10.496
DTASelect 10.306
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.672
IPC_peptide 10.687
IPC2_peptide 9.297
IPC2.peptide.svr19 8.601
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
147
0
147
64480
75
3161
438.6
49.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.368 ± 0.137
2.765 ± 0.095
4.994 ± 0.114
6.664 ± 0.191
3.916 ± 0.101
4.254 ± 0.125
3.015 ± 0.083
4.15 ± 0.086
5.538 ± 0.155
10.14 ± 0.18
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.985 ± 0.09
4.045 ± 0.102
5.625 ± 0.275
3.725 ± 0.101
5.642 ± 0.112
6.368 ± 0.14
6.239 ± 0.131
6.914 ± 0.121
1.058 ± 0.049
4.595 ± 0.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here