Cocksfoot mild mosaic virus
Average proteome isoelectric point is 7.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B4XRZ9|B4XRZ9_9TOMB Capsid protein OS=Cocksfoot mild mosaic virus OX=479060 PE=3 SV=1
MM1 pKa = 7.39 ATGTCHH7 pKa = 7.14 CPDD10 pKa = 4.22 NPFVWPLVTVVLILLIIIVASVTSTGPVIIPPSHH44 pKa = 5.74 NTTYY48 pKa = 10.55 HH49 pKa = 5.51 HH50 pKa = 6.79 EE51 pKa = 4.23 KK52 pKa = 8.99 YY53 pKa = 10.68 QNIEE57 pKa = 3.89 VQKK60 pKa = 11.04
Molecular weight: 6.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.86
IPC2_protein 5.97
IPC_protein 6.097
Toseland 6.504
ProMoST 6.547
Dawson 6.465
Bjellqvist 6.364
Wikipedia 6.491
Rodwell 6.453
Grimsley 6.649
Solomon 6.465
Lehninger 6.465
Nozaki 6.707
DTASelect 6.912
Thurlkill 6.942
EMBOSS 6.942
Sillero 6.854
Patrickios 0.846
IPC_peptide 6.491
IPC2_peptide 6.825
IPC2.peptide.svr19 6.71
Protein with the highest isoelectric point:
>tr|B4XS00|B4XS00_9TOMB p15 OS=Cocksfoot mild mosaic virus OX=479060 PE=4 SV=1
MM1 pKa = 7.57 NGGNTSNRR9 pKa = 11.84 RR10 pKa = 11.84 GRR12 pKa = 11.84 GKK14 pKa = 10.45 ALQRR18 pKa = 11.84 RR19 pKa = 11.84 TNKK22 pKa = 9.62 PPRR25 pKa = 11.84 SRR27 pKa = 11.84 SIEE30 pKa = 3.79 RR31 pKa = 11.84 ARR33 pKa = 11.84 PLAYY37 pKa = 9.78 GQAVGPSPPRR47 pKa = 11.84 TVAYY51 pKa = 10.26 RR52 pKa = 11.84 RR53 pKa = 11.84 GRR55 pKa = 11.84 QVNTQPEE62 pKa = 4.34 IILRR66 pKa = 11.84 ITAKK70 pKa = 8.32 TTDD73 pKa = 3.36 NYY75 pKa = 8.13 TTVPIIPALLFDD87 pKa = 5.41 PSTQPGYY94 pKa = 10.75 GGRR97 pKa = 11.84 AQYY100 pKa = 9.47 MAGLSQLHH108 pKa = 6.03 SQHH111 pKa = 4.99 EE112 pKa = 4.18 WRR114 pKa = 11.84 RR115 pKa = 11.84 LQFTWIPSCATVTPGNVVLKK135 pKa = 8.03 FFPNYY140 pKa = 7.63 RR141 pKa = 11.84 TPLPTVLEE149 pKa = 4.41 DD150 pKa = 5.3 LMDD153 pKa = 3.98 TSSLTFSPYY162 pKa = 9.96 EE163 pKa = 3.61 KK164 pKa = 9.45 HH165 pKa = 5.56 TFSVAGRR172 pKa = 11.84 ISGLKK177 pKa = 9.45 YY178 pKa = 10.78 NIGATAFLALSDD190 pKa = 4.23 EE191 pKa = 4.6 DD192 pKa = 3.99 KK193 pKa = 11.37 GDD195 pKa = 3.64 YY196 pKa = 10.75 SIGRR200 pKa = 11.84 LVIGTTSQVSAVSLGIIQMVPDD222 pKa = 3.94 VEE224 pKa = 4.58 FSGPVTTPVAPTPAATAAAPAA245 pKa = 4.11
Molecular weight: 26.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.706
IPC_protein 10.438
Toseland 10.204
ProMoST 10.043
Dawson 10.438
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 10.599
Grimsley 10.54
Solomon 10.496
Lehninger 10.452
Nozaki 10.189
DTASelect 10.189
Thurlkill 10.277
EMBOSS 10.628
Sillero 10.365
Patrickios 10.087
IPC_peptide 10.496
IPC2_peptide 9.121
IPC2.peptide.svr19 8.591
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1805
60
934
300.8
33.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.418 ± 1.145
1.994 ± 0.394
4.986 ± 0.522
4.654 ± 0.598
4.155 ± 0.483
5.817 ± 0.506
2.604 ± 0.489
4.598 ± 0.538
5.152 ± 1.04
9.197 ± 0.864
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.438 ± 0.237
3.546 ± 0.337
5.706 ± 1.097
3.934 ± 0.35
7.147 ± 0.5
7.036 ± 0.588
6.26 ± 1.19
5.873 ± 0.501
1.662 ± 0.368
3.767 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here