McMurdo Ice Shelf pond-associated circular DNA virus-5

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075M3Q1|A0A075M3Q1_9VIRU Putative capsid protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-5 OX=1521389 PE=4 SV=1
MM1 pKa = 7.82SDD3 pKa = 3.4FSEE6 pKa = 4.38YY7 pKa = 9.87TQISDD12 pKa = 3.45LSTYY16 pKa = 7.52EE17 pKa = 3.86WPSGSRR23 pKa = 11.84RR24 pKa = 11.84PPTEE28 pKa = 3.53NARR31 pKa = 11.84TTLDD35 pKa = 3.18EE36 pKa = 5.45LMNEE40 pKa = 4.44LLNIEE45 pKa = 4.28EE46 pKa = 5.06PEE48 pKa = 4.23LSHH51 pKa = 6.37EE52 pKa = 4.4TALLMNVYY60 pKa = 8.24TQEE63 pKa = 4.04TLLRR67 pKa = 11.84DD68 pKa = 3.68YY69 pKa = 11.45VNRR72 pKa = 11.84STWLAPEE79 pKa = 4.33DD80 pKa = 4.06LKK82 pKa = 11.52NALTSWYY89 pKa = 10.0YY90 pKa = 10.75HH91 pKa = 6.0MNIIWYY97 pKa = 8.51PSKK100 pKa = 10.04TPLTSTLSFLRR111 pKa = 11.84RR112 pKa = 11.84YY113 pKa = 7.64RR114 pKa = 11.84TSKK117 pKa = 10.57LGNNAQIRR125 pKa = 11.84YY126 pKa = 8.75LLLKK130 pKa = 10.04EE131 pKa = 4.03SHH133 pKa = 6.29LTLEE137 pKa = 4.35ARR139 pKa = 11.84EE140 pKa = 4.06QFYY143 pKa = 9.12MICQPEE149 pKa = 4.32THH151 pKa = 6.76ISHH154 pKa = 6.36YY155 pKa = 10.32PSTIQQNQASIPKK168 pKa = 9.74DD169 pKa = 3.43VQDD172 pKa = 4.26LVRR175 pKa = 11.84PVLKK179 pKa = 10.5SRR181 pKa = 11.84KK182 pKa = 7.03QTLNKK187 pKa = 10.14CPLTSLPISLQVRR200 pKa = 11.84AVGLFAIEE208 pKa = 4.67RR209 pKa = 11.84LTLPLASSLVVVTKK223 pKa = 10.33CSATLPTIPNCSQLFQKK240 pKa = 10.34QSCQNCQCCKK250 pKa = 8.07TTLSYY255 pKa = 11.04TRR257 pKa = 11.84TITYY261 pKa = 9.34HH262 pKa = 5.54

Molecular weight: 30.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075M3Q1|A0A075M3Q1_9VIRU Putative capsid protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-5 OX=1521389 PE=4 SV=1
MM1 pKa = 7.37SRR3 pKa = 11.84AKK5 pKa = 9.88HH6 pKa = 4.57WCFTVNNYY14 pKa = 8.25TDD16 pKa = 3.76EE17 pKa = 5.91DD18 pKa = 3.51IHH20 pKa = 7.87KK21 pKa = 10.23LSKK24 pKa = 10.81ASLLLQPLVSSCIYY38 pKa = 8.45QQEE41 pKa = 4.55VPGQEE46 pKa = 4.23SATPGTPHH54 pKa = 6.33LQGFISFKK62 pKa = 9.72TKK64 pKa = 10.47QSFKK68 pKa = 9.21FTKK71 pKa = 10.52NLVSDD76 pKa = 4.24RR77 pKa = 11.84AHH79 pKa = 6.78VEE81 pKa = 4.16VAKK84 pKa = 8.93GTPQQNRR91 pKa = 11.84IYY93 pKa = 10.38CSKK96 pKa = 11.11AKK98 pKa = 9.91DD99 pKa = 3.47RR100 pKa = 11.84KK101 pKa = 10.01IGTEE105 pKa = 3.7VFTYY109 pKa = 10.91GEE111 pKa = 4.17QPKK114 pKa = 10.28LLPGKK119 pKa = 10.59RR120 pKa = 11.84NDD122 pKa = 3.72LYY124 pKa = 11.48AFQQYY129 pKa = 9.21VKK131 pKa = 9.89EE132 pKa = 4.4GNIQTRR138 pKa = 11.84DD139 pKa = 3.1ILEE142 pKa = 4.01NHH144 pKa = 6.71ASVAARR150 pKa = 11.84YY151 pKa = 7.59PRR153 pKa = 11.84YY154 pKa = 8.67VRR156 pKa = 11.84EE157 pKa = 4.18YY158 pKa = 9.22IDD160 pKa = 5.59LYY162 pKa = 10.96VNPPEE167 pKa = 5.04VPDD170 pKa = 3.71HH171 pKa = 7.11PLYY174 pKa = 10.69LWQEE178 pKa = 4.26TLTKK182 pKa = 10.08IITGPSDD189 pKa = 3.2DD190 pKa = 3.7RR191 pKa = 11.84QIIFVVDD198 pKa = 3.47EE199 pKa = 4.42VGNQGKK205 pKa = 7.32TWFAKK210 pKa = 9.89KK211 pKa = 9.87YY212 pKa = 10.1CRR214 pKa = 11.84AHH216 pKa = 6.85EE217 pKa = 4.39DD218 pKa = 3.49AQFLEE223 pKa = 4.64PGKK226 pKa = 10.4KK227 pKa = 9.85ADD229 pKa = 3.33MAYY232 pKa = 10.74ALNTDD237 pKa = 3.52LRR239 pKa = 11.84VLFLNVTRR247 pKa = 11.84QQVEE251 pKa = 3.82HH252 pKa = 6.17LQYY255 pKa = 11.43SFLEE259 pKa = 4.15AVKK262 pKa = 10.56DD263 pKa = 3.99GSVWSPKK270 pKa = 9.36YY271 pKa = 10.17EE272 pKa = 4.01SRR274 pKa = 11.84TKK276 pKa = 10.57HH277 pKa = 5.12LTQIPHH283 pKa = 6.13IVVMMNQDD291 pKa = 3.58PDD293 pKa = 3.77FQLLSKK299 pKa = 10.69DD300 pKa = 3.63RR301 pKa = 11.84YY302 pKa = 7.02HH303 pKa = 7.33TIYY306 pKa = 10.38II307 pKa = 4.19

Molecular weight: 35.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

569

262

307

284.5

32.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.921 ± 0.465

2.109 ± 0.608

4.394 ± 1.109

5.8 ± 0.198

3.163 ± 0.808

2.812 ± 1.073

2.988 ± 0.449

5.097 ± 0.087

6.151 ± 1.257

10.721 ± 1.945

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.582 ± 0.21

4.394 ± 0.366

5.8 ± 0.198

6.678 ± 0.368

5.272 ± 0.292

7.557 ± 1.524

8.26 ± 1.563

5.624 ± 1.164

1.406 ± 0.078

5.272 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski