Aurantiacibacter zhengii
Average proteome isoelectric point is 5.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3511 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A418NS04|A0A418NS04_9SPHN YcgN family cysteine cluster protein OS=Aurantiacibacter zhengii OX=2307003 GN=D2V07_11150 PE=4 SV=1
MM1 pKa = 7.47 SARR4 pKa = 11.84 LSVVTPLLLALSLAACDD21 pKa = 4.38 GGGSEE26 pKa = 5.25 PPITDD31 pKa = 3.17 APLYY35 pKa = 10.54 NSALTGQYY43 pKa = 11.22 DD44 pKa = 3.9 LVDD47 pKa = 3.41 SSGAQVTNADD57 pKa = 3.61 FAGQYY62 pKa = 8.8 QLVYY66 pKa = 10.15 FGYY69 pKa = 10.26 AYY71 pKa = 10.82 CPDD74 pKa = 3.35 ICPFDD79 pKa = 3.97 MQRR82 pKa = 11.84 MMRR85 pKa = 11.84 GYY87 pKa = 11.14 DD88 pKa = 3.06 RR89 pKa = 11.84 FAEE92 pKa = 4.4 ANPDD96 pKa = 3.58 LADD99 pKa = 3.91 DD100 pKa = 3.94 VQPIFITIDD109 pKa = 3.41 PARR112 pKa = 11.84 DD113 pKa = 3.35 TPEE116 pKa = 3.93 KK117 pKa = 10.35 VGEE120 pKa = 4.06 YY121 pKa = 11.02 ANAFSDD127 pKa = 4.09 RR128 pKa = 11.84 LIGLTGSPEE137 pKa = 4.07 QIEE140 pKa = 4.02 AAAAAFFAHH149 pKa = 5.61 YY150 pKa = 10.25 QKK152 pKa = 10.99 LDD154 pKa = 3.49 PDD156 pKa = 4.16 AEE158 pKa = 4.36 SDD160 pKa = 3.75 YY161 pKa = 11.71 LMDD164 pKa = 4.43 HH165 pKa = 6.42 SRR167 pKa = 11.84 TGYY170 pKa = 10.18 LVDD173 pKa = 4.8 RR174 pKa = 11.84 EE175 pKa = 4.66 GEE177 pKa = 4.11 PMALIPVEE185 pKa = 4.72 DD186 pKa = 3.76 SAEE189 pKa = 3.9 AVAAEE194 pKa = 4.13 LEE196 pKa = 4.41 KK197 pKa = 10.22 WVRR200 pKa = 11.84 PADD203 pKa = 3.62 GG204 pKa = 3.71
Molecular weight: 22.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.91
IPC_protein 3.91
Toseland 3.694
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.253
Thurlkill 3.745
EMBOSS 3.834
Sillero 4.024
Patrickios 1.163
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.913
Protein with the highest isoelectric point:
>tr|A0A418NRI3|A0A418NRI3_9SPHN Glutathione S-transferase family protein OS=Aurantiacibacter zhengii OX=2307003 GN=D2V07_09945 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 8.02 ATVGGRR28 pKa = 11.84 KK29 pKa = 8.07 VLRR32 pKa = 11.84 NRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.0 KK41 pKa = 10.54 LSAA44 pKa = 4.03
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3511
0
3511
1132093
30
12886
322.4
35.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.342 ± 0.068
0.833 ± 0.017
6.329 ± 0.046
6.422 ± 0.041
3.669 ± 0.027
8.834 ± 0.047
1.928 ± 0.026
4.914 ± 0.03
2.881 ± 0.035
9.692 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.543 ± 0.034
2.76 ± 0.03
5.063 ± 0.029
3.296 ± 0.02
6.936 ± 0.052
5.473 ± 0.038
5.252 ± 0.048
7.107 ± 0.037
1.459 ± 0.019
2.267 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here