Rheinheimera phage vB_RspM_Barba31A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Barbavirus; unclassified Barbavirus

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 143 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8NKH8|A0A4P8NKH8_9CAUD DNA primase OS=Rheinheimera phage vB_RspM_Barba31A OX=2565682 GN=Barba31A_gp027 PE=4 SV=1
MM1 pKa = 7.32FRR3 pKa = 11.84FSNQPIVSSKK13 pKa = 10.44FVFGVGNFGVLYY25 pKa = 10.45DD26 pKa = 3.86VVPTTGQNGAAVLLNDD42 pKa = 3.99GGVNGDD48 pKa = 3.75YY49 pKa = 10.92VRR51 pKa = 11.84LRR53 pKa = 11.84IDD55 pKa = 4.4SIDD58 pKa = 3.54PTITSLFVYY67 pKa = 9.9EE68 pKa = 4.96DD69 pKa = 3.87GSFEE73 pKa = 4.46SQGTGQWTYY82 pKa = 10.37YY83 pKa = 9.72YY84 pKa = 10.48SEE86 pKa = 4.43NGIEE90 pKa = 4.37NPTLNTVTINPFVTDD105 pKa = 3.68SNLTISDD112 pKa = 3.93VLPALSSSVTITNVVPEE129 pKa = 4.39YY130 pKa = 10.51NLSISDD136 pKa = 3.97SFSPLQSSMSLVNGIPEE153 pKa = 4.27SSLSIATTLPSLSSSVSIEE172 pKa = 4.0NVVPTYY178 pKa = 11.13SLAITTSLPSLQSTVGLSYY197 pKa = 11.25EE198 pKa = 4.11DD199 pKa = 3.99ALYY202 pKa = 10.71DD203 pKa = 3.55ITISDD208 pKa = 4.81NLPSLQSAITLEE220 pKa = 4.48NIVPVYY226 pKa = 9.62TLEE229 pKa = 4.14ITDD232 pKa = 4.23SFAALQSSVNIINGEE247 pKa = 4.07LTIHH251 pKa = 6.52VAPQNLIYY259 pKa = 10.36IKK261 pKa = 9.91PKK263 pKa = 9.84PRR265 pKa = 11.84FITLRR270 pKa = 3.64

Molecular weight: 29.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8NKL5|A0A4P8NKL5_9CAUD Uncharacterized protein OS=Rheinheimera phage vB_RspM_Barba31A OX=2565682 GN=Barba31A_gp087 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.37RR3 pKa = 11.84KK4 pKa = 10.04LIQRR8 pKa = 11.84NKK10 pKa = 9.23QFLSSRR16 pKa = 11.84YY17 pKa = 7.63KK18 pKa = 10.62HH19 pKa = 6.26NYY21 pKa = 8.31SGNGSFQRR29 pKa = 11.84IRR31 pKa = 11.84VHH33 pKa = 6.8KK34 pKa = 9.94PFIDD38 pKa = 3.47SHH40 pKa = 7.9GEE42 pKa = 3.66LQIRR46 pKa = 11.84KK47 pKa = 8.55PRR49 pKa = 11.84YY50 pKa = 6.44IWRR53 pKa = 11.84WFFNAEE59 pKa = 3.87WFSDD63 pKa = 3.7LVGWFSDD70 pKa = 3.94LRR72 pKa = 11.84GEE74 pKa = 4.37VHH76 pKa = 7.43DD77 pKa = 4.56

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

143

0

143

25404

24

950

177.7

20.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.204 ± 0.307

1.48 ± 0.104

6.405 ± 0.152

6.66 ± 0.222

4.326 ± 0.141

6.574 ± 0.214

1.657 ± 0.109

5.987 ± 0.187

6.873 ± 0.27

8.455 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.113

5.511 ± 0.22

3.263 ± 0.157

3.826 ± 0.132

3.999 ± 0.138

6.656 ± 0.193

6.408 ± 0.281

7.227 ± 0.209

1.46 ± 0.098

4.562 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski