Urbifossiella limnaea

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Gemmatales; Gemmataceae; Urbifossiella

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6172 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517Y3M4|A0A517Y3M4_9BACT Penicillin acylase OS=Urbifossiella limnaea OX=2528023 GN=ETAA1_63420 PE=3 SV=1
MM1 pKa = 6.87TTLLCRR7 pKa = 11.84LWDD10 pKa = 4.16DD11 pKa = 4.23DD12 pKa = 4.11RR13 pKa = 11.84GAVISSEE20 pKa = 4.5LILVLGVLVMGLIPGLVALRR40 pKa = 11.84NSTNSALTTIGNFLGRR56 pKa = 11.84ITPSFTYY63 pKa = 9.2SGYY66 pKa = 10.14MILPEE71 pKa = 4.45GGISTVAVVGGLQVDD86 pKa = 4.12YY87 pKa = 8.27TTANQLSAAQVEE99 pKa = 4.57PVDD102 pKa = 3.89TDD104 pKa = 3.66FEE106 pKa = 4.95YY107 pKa = 10.36PGPFVVSPAPP117 pKa = 3.36

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517XWE7|A0A517XWE7_9BACT Uncharacterized protein OS=Urbifossiella limnaea OX=2528023 GN=ETAA1_37670 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84VPSPFWISRR11 pKa = 11.84AIRR14 pKa = 11.84FNLPLSVVSSFGVGWSKK31 pKa = 10.68RR32 pKa = 11.84RR33 pKa = 11.84GWVLVFGWMSRR44 pKa = 11.84SASSIFF50 pKa = 3.34

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6172

0

6172

2292864

29

15717

371.5

39.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.565 ± 0.058

0.932 ± 0.013

6.074 ± 0.021

4.991 ± 0.041

3.524 ± 0.018

9.235 ± 0.056

2.029 ± 0.023

2.709 ± 0.025

3.306 ± 0.041

9.838 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.585 ± 0.017

2.315 ± 0.029

6.795 ± 0.042

2.564 ± 0.016

7.83 ± 0.061

4.169 ± 0.042

5.924 ± 0.069

8.826 ± 0.04

1.514 ± 0.016

2.276 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski