Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae; Geobacter; Geobacter sulfurreducens

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3402 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q74CS5|Q74CS5_GEOSL Uncharacterized protein OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=GSU1597 PE=4 SV=1
MM1 pKa = 7.77EE2 pKa = 5.55EE3 pKa = 4.12SQKK6 pKa = 10.31QPRR9 pKa = 11.84LCSEE13 pKa = 3.54IQLFDD18 pKa = 3.62LCEE21 pKa = 3.84VGAADD26 pKa = 4.08VCGHH30 pKa = 6.75RR31 pKa = 11.84DD32 pKa = 3.08GRR34 pKa = 11.84YY35 pKa = 6.86CTKK38 pKa = 10.82GDD40 pKa = 3.48LLARR44 pKa = 11.84FEE46 pKa = 5.67AIADD50 pKa = 3.83DD51 pKa = 4.34DD52 pKa = 4.78RR53 pKa = 11.84SPEE56 pKa = 3.78QYY58 pKa = 10.12FAEE61 pKa = 4.44EE62 pKa = 4.14LDD64 pKa = 4.0DD65 pKa = 6.06AEE67 pKa = 4.73GADD70 pKa = 3.94DD71 pKa = 5.04LGYY74 pKa = 11.09DD75 pKa = 3.44EE76 pKa = 6.11AFGVDD81 pKa = 3.58EE82 pKa = 4.48YY83 pKa = 11.84GEE85 pKa = 4.11EE86 pKa = 4.34DD87 pKa = 3.67EE88 pKa = 4.82EE89 pKa = 4.5

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q746R5|DCUP_GEOSL Uroporphyrinogen decarboxylase OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=hemE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.32QPSNTSRR12 pKa = 11.84KK13 pKa = 7.49RR14 pKa = 11.84THH16 pKa = 6.14GFLVRR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84LVIKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.4RR41 pKa = 11.84LSVSIAGKK49 pKa = 10.2

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3402

0

3402

1144256

20

5899

336.3

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.289 ± 0.054

1.246 ± 0.025

5.361 ± 0.034

6.51 ± 0.049

3.922 ± 0.025

8.444 ± 0.039

2.054 ± 0.023

5.589 ± 0.039

4.082 ± 0.044

10.168 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.021

2.926 ± 0.033

4.697 ± 0.038

2.776 ± 0.021

7.022 ± 0.061

5.491 ± 0.044

5.607 ± 0.07

7.788 ± 0.036

1.002 ± 0.015

2.629 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski