Mycobacterium phage LittleCherry

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5Y3A4|S5Y3A4_9CAUD Uncharacterized protein OS=Mycobacterium phage LittleCherry OX=1340818 GN=84 PE=4 SV=1
MM1 pKa = 7.24ARR3 pKa = 11.84IYY5 pKa = 10.83VADD8 pKa = 4.16LAAYY12 pKa = 10.34NEE14 pKa = 4.16GHH16 pKa = 7.21LVGAWIDD23 pKa = 3.65LEE25 pKa = 4.87AYY27 pKa = 10.48SGDD30 pKa = 3.98LEE32 pKa = 4.26ALEE35 pKa = 4.58AAVKK39 pKa = 10.41AVLLDD44 pKa = 4.4GNEE47 pKa = 3.6EE48 pKa = 3.99WAIHH52 pKa = 6.47DD53 pKa = 4.62DD54 pKa = 3.72EE55 pKa = 5.77GFGNIRR61 pKa = 11.84IFEE64 pKa = 4.33HH65 pKa = 7.04EE66 pKa = 4.58DD67 pKa = 3.08LGTLLDD73 pKa = 3.95LSEE76 pKa = 5.87AIEE79 pKa = 3.96KK80 pKa = 10.79HH81 pKa = 5.65GDD83 pKa = 3.5PFVAWLGEE91 pKa = 4.23VVGDD95 pKa = 3.34ICYY98 pKa = 9.4FANVADD104 pKa = 4.75AVDD107 pKa = 3.74QFEE110 pKa = 4.32DD111 pKa = 4.74RR112 pKa = 11.84YY113 pKa = 9.75VGPMTLEE120 pKa = 4.42DD121 pKa = 3.62YY122 pKa = 10.46AYY124 pKa = 9.72EE125 pKa = 4.09YY126 pKa = 11.37AEE128 pKa = 4.28EE129 pKa = 4.09VLGLKK134 pKa = 8.88DD135 pKa = 3.13TALDD139 pKa = 3.52YY140 pKa = 11.35FNYY143 pKa = 10.44EE144 pKa = 3.76KK145 pKa = 10.57FANDD149 pKa = 3.35LALSGEE155 pKa = 4.39MVEE158 pKa = 4.18VWYY161 pKa = 10.43EE162 pKa = 3.69GVDD165 pKa = 3.55YY166 pKa = 11.04VFRR169 pKa = 11.84AWW171 pKa = 3.34

Molecular weight: 19.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5XWM1|S5XWM1_9CAUD Uncharacterized protein OS=Mycobacterium phage LittleCherry OX=1340818 GN=61 PE=4 SV=1
MM1 pKa = 7.83PGPMSAPNGNGLITVSPEE19 pKa = 3.36ARR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 9.95AGRR26 pKa = 11.84TGKK29 pKa = 8.52TEE31 pKa = 3.87LVNEE35 pKa = 4.14RR36 pKa = 11.84KK37 pKa = 9.13EE38 pKa = 3.98RR39 pKa = 11.84KK40 pKa = 9.25AGKK43 pKa = 9.63KK44 pKa = 9.48

Molecular weight: 4.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

15811

27

1112

181.7

20.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.335 ± 0.311

0.923 ± 0.125

7.014 ± 0.202

7.356 ± 0.288

3.422 ± 0.221

8.462 ± 0.504

1.948 ± 0.149

4.933 ± 0.177

4.668 ± 0.331

8.254 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.296 ± 0.119

3.27 ± 0.144

5.085 ± 0.276

3.358 ± 0.214

6.141 ± 0.306

5.25 ± 0.217

5.831 ± 0.233

7.507 ± 0.258

1.973 ± 0.147

2.973 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski