Janthinobacterium sp. B9-8
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4080 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A120II39|A0A120II39_9BURK Uncharacterized protein OS=Janthinobacterium sp. B9-8 OX=1236179 GN=VN23_15720 PE=4 SV=1
MM1 pKa = 6.87 NTVTDD6 pKa = 3.77 MPIPFVFTDD15 pKa = 3.78 SAAEE19 pKa = 4.02 KK20 pKa = 10.73 VRR22 pKa = 11.84 DD23 pKa = 4.06 LIIEE27 pKa = 4.3 EE28 pKa = 4.57 GNPDD32 pKa = 3.03 LKK34 pKa = 11.14 LRR36 pKa = 11.84 VFVTGGGCSGFQYY49 pKa = 10.94 GFTFDD54 pKa = 4.81 EE55 pKa = 4.64 ITNEE59 pKa = 4.02 DD60 pKa = 3.73 DD61 pKa = 4.08 TPVEE65 pKa = 4.3 KK66 pKa = 11.1 NGVTLLVDD74 pKa = 3.69 PMSYY78 pKa = 10.34 QYY80 pKa = 11.57 LVGAEE85 pKa = 3.69 IDD87 pKa = 3.85 YY88 pKa = 11.28 VEE90 pKa = 4.54 SLEE93 pKa = 4.67 GSQFTIKK100 pKa = 10.58 NPNASTTCGCGSSFSVV116 pKa = 3.54
Molecular weight: 12.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.897
IPC_protein 3.821
Toseland 3.63
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.541
Solomon 3.783
Lehninger 3.745
Nozaki 3.935
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.948
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|A0A0Y0USV9|A0A0Y0USV9_9BURK Cobyric acid synthase OS=Janthinobacterium sp. B9-8 OX=1236179 GN=VN23_01805 PE=4 SV=1
MM1 pKa = 7.41 VLFIIIFCGFLLFEE15 pKa = 4.77 LVLGNLPWWIAGGYY29 pKa = 10.6 LLMSTVAFIAYY40 pKa = 10.05 ALDD43 pKa = 3.54 KK44 pKa = 11.07 SAARR48 pKa = 11.84 NNRR51 pKa = 11.84 WRR53 pKa = 11.84 TPEE56 pKa = 3.92 RR57 pKa = 11.84 TLHH60 pKa = 6.05 LLGVLGGWRR69 pKa = 11.84 GALLAQRR76 pKa = 11.84 VLRR79 pKa = 11.84 HH80 pKa = 5.86 KK81 pKa = 9.72 SAKK84 pKa = 9.84 VVFLRR89 pKa = 11.84 VFVATIVLNCAALACLLAVGGFWQGG114 pKa = 2.77
Molecular weight: 12.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.692
IPC_protein 10.584
Toseland 10.643
ProMoST 10.394
Dawson 10.76
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.906
Grimsley 10.818
Solomon 10.891
Lehninger 10.862
Nozaki 10.687
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.701
Patrickios 10.716
IPC_peptide 10.891
IPC2_peptide 9.882
IPC2.peptide.svr19 8.418
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4080
0
4080
1323239
37
4936
324.3
35.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.892 ± 0.061
0.976 ± 0.014
5.14 ± 0.032
5.535 ± 0.046
3.901 ± 0.031
7.221 ± 0.057
2.152 ± 0.022
5.881 ± 0.03
4.769 ± 0.044
11.25 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.529 ± 0.024
3.678 ± 0.036
4.335 ± 0.032
4.529 ± 0.038
5.06 ± 0.045
6.53 ± 0.044
5.001 ± 0.067
6.64 ± 0.041
1.353 ± 0.02
2.628 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here