Pseudovibrio axinellae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Stappiaceae; Pseudovibrio

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4634 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A165WXM8|A0A165WXM8_9RHOB Type I secretion system ATP-binding protein PrsD OS=Pseudovibrio axinellae OX=989403 GN=prsD_4 PE=4 SV=1
MM1 pKa = 7.5NIKK4 pKa = 8.67TLALAASAAAFATSAQAADD23 pKa = 4.16LPMAAEE29 pKa = 3.99PVDD32 pKa = 3.81YY33 pKa = 11.22VKK35 pKa = 11.11ACDD38 pKa = 3.72AFGAGYY44 pKa = 9.0FQLPGKK50 pKa = 7.03EE51 pKa = 4.1TCIKK55 pKa = 10.23LGGRR59 pKa = 11.84IRR61 pKa = 11.84AQYY64 pKa = 10.59VSQDD68 pKa = 3.66LTDD71 pKa = 3.76KK72 pKa = 10.84STDD75 pKa = 3.33NDD77 pKa = 3.36ATSYY81 pKa = 10.61ARR83 pKa = 11.84GYY85 pKa = 10.49IYY87 pKa = 10.25FNSMTATDD95 pKa = 4.6FGTIKK100 pKa = 10.02TFVEE104 pKa = 5.69LEE106 pKa = 4.31SEE108 pKa = 4.29WNQDD112 pKa = 3.96AEE114 pKa = 4.76GADD117 pKa = 3.57TKK119 pKa = 11.74ANDD122 pKa = 2.79VWIQLGTGYY131 pKa = 11.14GSFLFGRR138 pKa = 11.84EE139 pKa = 3.5ASAFDD144 pKa = 3.96AFTGYY149 pKa = 7.61TWIGPVGNAYY159 pKa = 10.76SDD161 pKa = 3.54TSTLQASFTADD172 pKa = 3.17LGNGLTATASVEE184 pKa = 3.92DD185 pKa = 3.56SAYY188 pKa = 10.6RR189 pKa = 11.84AGDD192 pKa = 3.37NDD194 pKa = 3.64AVDD197 pKa = 4.76FVGALNLSQGWGSFKK212 pKa = 10.59LAAAAHH218 pKa = 5.22NTANSEE224 pKa = 4.2DD225 pKa = 3.59YY226 pKa = 10.68GYY228 pKa = 11.47AVGATAIFNLDD239 pKa = 3.29MVKK242 pKa = 10.53EE243 pKa = 4.31GTEE246 pKa = 3.93VTFQAQYY253 pKa = 11.7ADD255 pKa = 3.69DD256 pKa = 4.42AGNYY260 pKa = 9.36IGIDD264 pKa = 3.46TDD266 pKa = 3.68EE267 pKa = 6.08DD268 pKa = 3.67DD269 pKa = 3.59VDD271 pKa = 3.7AVRR274 pKa = 11.84GYY276 pKa = 10.59SLSAGLEE283 pKa = 3.91TALTDD288 pKa = 3.97KK289 pKa = 11.18VSAQLDD295 pKa = 3.99LSYY298 pKa = 10.56MDD300 pKa = 4.08IEE302 pKa = 4.66STVSGSDD309 pKa = 3.25EE310 pKa = 4.91DD311 pKa = 3.95EE312 pKa = 3.55QTYY315 pKa = 10.62AVNGSVVYY323 pKa = 10.68SPVEE327 pKa = 3.91GLGLALAAGWSDD339 pKa = 3.7GEE341 pKa = 4.07EE342 pKa = 4.26DD343 pKa = 5.56GVDD346 pKa = 3.41KK347 pKa = 11.16DD348 pKa = 5.33DD349 pKa = 3.88EE350 pKa = 4.88VKK352 pKa = 10.86VGARR356 pKa = 11.84VQYY359 pKa = 9.56TFF361 pKa = 4.12

Molecular weight: 38.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165ULJ6|A0A165ULJ6_9RHOB Nucleoside diphosphate kinase OS=Pseudovibrio axinellae OX=989403 GN=ndk PE=3 SV=1
MM1 pKa = 7.44LRR3 pKa = 11.84TATLQLMLSSRR14 pKa = 11.84NSRR17 pKa = 11.84EE18 pKa = 3.83RR19 pKa = 11.84ANGAVLPEE27 pKa = 3.85YY28 pKa = 8.98RR29 pKa = 11.84TLSNCVRR36 pKa = 11.84LFTRR40 pKa = 11.84QAQMTAGQRR49 pKa = 11.84FLEE52 pKa = 4.48SLLRR56 pKa = 11.84WKK58 pKa = 9.6STVSAMAKK66 pKa = 9.59YY67 pKa = 9.02VYY69 pKa = 10.5CMKK72 pKa = 10.79CEE74 pKa = 3.73DD75 pKa = 4.22AIRR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84SLTVCCVACAKK91 pKa = 10.41AAISSTVPTMFLRR104 pKa = 11.84ALGFPLRR111 pKa = 11.84TTHH114 pKa = 7.37CSGSPLKK121 pKa = 10.47KK122 pKa = 9.95QAATTIAPRR131 pKa = 11.84PLLQCWDD138 pKa = 3.27QRR140 pKa = 11.84LLVGG144 pKa = 4.83

Molecular weight: 16.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4634

0

4634

1443537

29

3466

311.5

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.171 ± 0.041

1.006 ± 0.014

5.463 ± 0.032

6.339 ± 0.037

4.107 ± 0.029

7.819 ± 0.038

2.093 ± 0.02

5.705 ± 0.03

4.59 ± 0.033

10.196 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.019

3.368 ± 0.022

4.513 ± 0.023

3.703 ± 0.024

5.505 ± 0.037

6.422 ± 0.031

5.405 ± 0.03

7.175 ± 0.027

1.277 ± 0.014

2.558 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski