Avon-Heathcote Estuary associated circular virus 27

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IBU2|A0A0C5IBU2_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 27 OX=1618251 PE=4 SV=1
MM1 pKa = 7.59PAKK4 pKa = 10.08QAFNMPFTAFDD15 pKa = 3.63VEE17 pKa = 4.53DD18 pKa = 4.43IEE20 pKa = 4.78WKK22 pKa = 9.06PDD24 pKa = 2.87HH25 pKa = 6.56MKK27 pKa = 10.43YY28 pKa = 10.61LLYY31 pKa = 10.78GRR33 pKa = 11.84EE34 pKa = 4.27TCPSTGRR41 pKa = 11.84EE42 pKa = 3.89HH43 pKa = 6.56LQGYY47 pKa = 6.99VQFIKK52 pKa = 10.59KK53 pKa = 7.28QTIAGAKK60 pKa = 9.9KK61 pKa = 9.86IFDD64 pKa = 3.45NATIHH69 pKa = 6.96FEE71 pKa = 4.09FALGDD76 pKa = 3.53YY77 pKa = 10.46EE78 pKa = 5.86EE79 pKa = 4.24NFKK82 pKa = 11.34YY83 pKa = 9.28CTKK86 pKa = 10.36TRR88 pKa = 11.84VNDD91 pKa = 3.62MVPNVYY97 pKa = 10.06FEE99 pKa = 4.58RR100 pKa = 11.84GEE102 pKa = 3.93PTRR105 pKa = 11.84KK106 pKa = 9.58KK107 pKa = 10.47GQRR110 pKa = 11.84NDD112 pKa = 2.9IQEE115 pKa = 4.04VIRR118 pKa = 11.84RR119 pKa = 11.84IEE121 pKa = 4.02SGNDD125 pKa = 3.0LRR127 pKa = 11.84DD128 pKa = 3.36LMFDD132 pKa = 3.8DD133 pKa = 5.24EE134 pKa = 5.03VNQTVARR141 pKa = 11.84CMPFVRR147 pKa = 11.84QAIADD152 pKa = 4.64FKK154 pKa = 11.09AGKK157 pKa = 9.41GLSAIKK163 pKa = 10.47SKK165 pKa = 11.27LEE167 pKa = 3.92SAQLRR172 pKa = 11.84SWQHH176 pKa = 4.24GVKK179 pKa = 10.29SLVEE183 pKa = 3.9QEE185 pKa = 3.98PEE187 pKa = 3.91GRR189 pKa = 11.84SVYY192 pKa = 9.83WYY194 pKa = 9.46WDD196 pKa = 3.4TKK198 pKa = 11.19GNTGKK203 pKa = 10.7SFMVDD208 pKa = 2.38WLVAFHH214 pKa = 6.65KK215 pKa = 10.78AVVFTHH221 pKa = 6.24GKK223 pKa = 7.14MHH225 pKa = 7.6DD226 pKa = 3.17IAHH229 pKa = 7.38AYY231 pKa = 8.11NHH233 pKa = 6.14EE234 pKa = 4.18KK235 pKa = 10.4VVCFDD240 pKa = 3.99LSRR243 pKa = 11.84TQEE246 pKa = 3.91EE247 pKa = 4.53KK248 pKa = 11.09LDD250 pKa = 3.8AVYY253 pKa = 9.14MALEE257 pKa = 4.23CFKK260 pKa = 10.65NGRR263 pKa = 11.84MFSGKK268 pKa = 10.08YY269 pKa = 9.81EE270 pKa = 4.09STTKK274 pKa = 10.21IFEE277 pKa = 4.32VPHH280 pKa = 5.73VFVFANFAPDD290 pKa = 3.36KK291 pKa = 10.22TKK293 pKa = 10.85LSEE296 pKa = 4.39DD297 pKa = 2.93RR298 pKa = 11.84WKK300 pKa = 9.29VTEE303 pKa = 4.75IISFF307 pKa = 3.92

Molecular weight: 35.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IBU2|A0A0C5IBU2_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 27 OX=1618251 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.18RR3 pKa = 11.84ARR5 pKa = 11.84DD6 pKa = 3.77YY7 pKa = 10.96QRR9 pKa = 11.84PRR11 pKa = 11.84YY12 pKa = 8.85SKK14 pKa = 10.01RR15 pKa = 11.84SRR17 pKa = 11.84RR18 pKa = 11.84PTPQRR23 pKa = 11.84SMRR26 pKa = 11.84SVIGRR31 pKa = 11.84LAEE34 pKa = 4.13SKK36 pKa = 10.66RR37 pKa = 11.84EE38 pKa = 3.91NFHH41 pKa = 7.13VSSQTLNHH49 pKa = 7.03DD50 pKa = 3.9AGSGQVVGGFARR62 pKa = 11.84PNNRR66 pKa = 11.84SGFNWMGLNQGDD78 pKa = 4.36SKK80 pKa = 11.07HH81 pKa = 6.86DD82 pKa = 3.81RR83 pKa = 11.84DD84 pKa = 3.68GDD86 pKa = 4.54SIFSQYY92 pKa = 9.93IDD94 pKa = 3.86YY95 pKa = 10.83KK96 pKa = 10.87LQLTGYY102 pKa = 9.69SDD104 pKa = 4.46YY105 pKa = 10.77PSQSCRR111 pKa = 11.84VIIFTPKK118 pKa = 10.45ALRR121 pKa = 11.84TNTLIASSNVASLFVTDD138 pKa = 5.28GSDD141 pKa = 3.29SGSGNLLIQPVDD153 pKa = 3.42TFNYY157 pKa = 9.55NVLRR161 pKa = 11.84DD162 pKa = 4.12FVVHH166 pKa = 6.35PLKK169 pKa = 10.88NQNQNSSAAQSTGLLYY185 pKa = 10.67DD186 pKa = 3.54VLHH189 pKa = 6.75PSISSGPFTSVAGTYY204 pKa = 9.86RR205 pKa = 11.84EE206 pKa = 4.48LFNKK210 pKa = 8.76YY211 pKa = 10.06HH212 pKa = 6.31SIIYY216 pKa = 10.22NEE218 pKa = 4.09LPALEE223 pKa = 4.54SAAPTLAPYY232 pKa = 9.93IAAKK236 pKa = 9.93IAEE239 pKa = 4.18SQYY242 pKa = 11.06YY243 pKa = 8.84INEE246 pKa = 4.12YY247 pKa = 10.15VDD249 pKa = 5.63DD250 pKa = 3.86VTEE253 pKa = 3.98YY254 pKa = 11.58SNIPFDD260 pKa = 4.29PVDD263 pKa = 4.87DD264 pKa = 5.18AGNDD268 pKa = 3.38CNYY271 pKa = 9.54PIVSGRR277 pKa = 11.84IKK279 pKa = 9.59TNRR282 pKa = 11.84RR283 pKa = 11.84VQYY286 pKa = 8.12EE287 pKa = 4.17TDD289 pKa = 3.07SVRR292 pKa = 11.84PLKK295 pKa = 8.62TTDD298 pKa = 4.6FIQIAIIPYY307 pKa = 9.14ADD309 pKa = 3.84PSAVTTTTIMRR320 pKa = 11.84YY321 pKa = 6.79NQEE324 pKa = 3.61VTHH327 pKa = 5.66YY328 pKa = 10.43FKK330 pKa = 11.21DD331 pKa = 3.91FF332 pKa = 3.52

Molecular weight: 37.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

639

307

332

319.5

36.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.573 ± 0.185

1.095 ± 0.368

6.573 ± 0.265

5.321 ± 1.723

5.79 ± 1.174

5.477 ± 0.042

2.66 ± 0.412

5.477 ± 0.633

6.26 ± 1.974

5.477 ± 0.408

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.736

5.321 ± 0.974

4.538 ± 0.884

4.382 ± 0.326

6.103 ± 0.615

7.668 ± 2.145

6.103 ± 0.39

6.886 ± 0.418

1.095 ± 0.593

5.008 ± 0.983
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski