Sparus aurata polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2RW94|A0A1B2RW94_9POLY ORF1 OS=Sparus aurata polyomavirus 1 OX=1885927 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.63IEE4 pKa = 4.63EE5 pKa = 4.24KK6 pKa = 10.62LKK8 pKa = 10.76RR9 pKa = 11.84ALGSCYY15 pKa = 7.28TTQVSIPQNMEE26 pKa = 4.02LFRR29 pKa = 11.84QAQMLEE35 pKa = 4.3GSQPDD40 pKa = 3.67EE41 pKa = 4.71VYY43 pKa = 9.98VQQVEE48 pKa = 4.25EE49 pKa = 5.36LYY51 pKa = 10.67KK52 pKa = 10.58QWTEE56 pKa = 3.76YY57 pKa = 9.44TAVVQTIAATTPQKK71 pKa = 10.53KK72 pKa = 10.39GPDD75 pKa = 3.71VFCNDD80 pKa = 3.57QVQDD84 pKa = 3.71YY85 pKa = 9.69CDD87 pKa = 3.19TVAAVVNQVVVMCPTEE103 pKa = 4.14RR104 pKa = 11.84APHH107 pKa = 6.54LSRR110 pKa = 11.84IIFSYY115 pKa = 10.73KK116 pKa = 10.38NEE118 pKa = 4.0DD119 pKa = 3.79KK120 pKa = 10.93FWVDD124 pKa = 3.31MNDD127 pKa = 3.63EE128 pKa = 4.12KK129 pKa = 10.59WGKK132 pKa = 9.71VSYY135 pKa = 10.39IGAKK139 pKa = 8.65FRR141 pKa = 11.84YY142 pKa = 8.85RR143 pKa = 11.84IVRR146 pKa = 11.84EE147 pKa = 3.54VLFRR151 pKa = 11.84KK152 pKa = 10.23FKK154 pKa = 9.46MSCGADD160 pKa = 2.87SWFVGMPVKK169 pKa = 10.4SGKK172 pKa = 10.48LSMVKK177 pKa = 10.49SMLLGSSCVEE187 pKa = 3.97KK188 pKa = 10.91VVEE191 pKa = 4.34GQLSFEE197 pKa = 4.53TPPFLQAMLSLYY209 pKa = 10.72AEE211 pKa = 4.38EE212 pKa = 5.56HH213 pKa = 6.72SITDD217 pKa = 3.55PFLLMGQYY225 pKa = 8.98MRR227 pKa = 11.84LADD230 pKa = 5.31DD231 pKa = 3.66SPEE234 pKa = 3.8VCRR237 pKa = 11.84FCDD240 pKa = 3.32EE241 pKa = 3.8EE242 pKa = 4.61RR243 pKa = 11.84RR244 pKa = 11.84MYY246 pKa = 10.57RR247 pKa = 11.84GIEE250 pKa = 3.91NEE252 pKa = 3.81SKK254 pKa = 10.2RR255 pKa = 11.84RR256 pKa = 11.84HH257 pKa = 5.24NRR259 pKa = 11.84NHH261 pKa = 5.57QKK263 pKa = 10.34HH264 pKa = 4.92YY265 pKa = 10.76QNAKK269 pKa = 10.22GFMALHH275 pKa = 6.32NKK277 pKa = 8.9KK278 pKa = 9.35QACGFATDD286 pKa = 3.46VVMGRR291 pKa = 11.84LRR293 pKa = 11.84VSSCGTRR300 pKa = 11.84DD301 pKa = 3.24EE302 pKa = 4.65RR303 pKa = 11.84FQASVLAHH311 pKa = 6.72FGTLRR316 pKa = 11.84DD317 pKa = 4.0MGCLSQNQVAAAYY330 pKa = 9.97LLANLIPVSVVHH342 pKa = 6.83FYY344 pKa = 10.27TYY346 pKa = 11.05VVGRR350 pKa = 11.84MLKK353 pKa = 10.17AVPKK357 pKa = 8.54QQGFIFFGAMNTGKK371 pKa = 8.56STVASALCDD380 pKa = 4.24FMSGCALNVNCPRR393 pKa = 11.84DD394 pKa = 3.87RR395 pKa = 11.84LWVEE399 pKa = 3.71MGRR402 pKa = 11.84CIDD405 pKa = 3.13RR406 pKa = 11.84FMVVFEE412 pKa = 4.95DD413 pKa = 3.88VMGVPMRR420 pKa = 11.84GHH422 pKa = 6.88TYY424 pKa = 10.52LALGDD429 pKa = 3.9GMSNLDD435 pKa = 3.62YY436 pKa = 10.59MRR438 pKa = 11.84EE439 pKa = 4.01HH440 pKa = 7.33LDD442 pKa = 3.31GLFKK446 pKa = 11.06VGLEE450 pKa = 3.77RR451 pKa = 11.84KK452 pKa = 8.57HH453 pKa = 5.59QNKK456 pKa = 10.01LDD458 pKa = 3.6MYY460 pKa = 8.64FPPWVVTCNHH470 pKa = 5.45YY471 pKa = 10.78VIPDD475 pKa = 4.01SIKK478 pKa = 9.57QRR480 pKa = 11.84SLSVEE485 pKa = 4.39FWSKK489 pKa = 10.68KK490 pKa = 8.32YY491 pKa = 10.25SVRR494 pKa = 11.84DD495 pKa = 3.75FCKK498 pKa = 10.24KK499 pKa = 9.11WDD501 pKa = 3.29IDD503 pKa = 4.09LRR505 pKa = 11.84FLSSGPCMLLMFALLGDD522 pKa = 4.08EE523 pKa = 4.13EE524 pKa = 4.56WFIEE528 pKa = 4.65QEE530 pKa = 4.12LEE532 pKa = 4.2TVKK535 pKa = 9.33MWKK538 pKa = 10.34DD539 pKa = 3.35EE540 pKa = 4.18AKK542 pKa = 10.84HH543 pKa = 6.18FDD545 pKa = 4.05TDD547 pKa = 4.36SMTTDD552 pKa = 3.28MEE554 pKa = 4.84TEE556 pKa = 4.07GEE558 pKa = 3.97PDD560 pKa = 3.72IEE562 pKa = 4.14IQEE565 pKa = 4.7DD566 pKa = 4.05GTLLFTDD573 pKa = 5.51GPSISGTMIPSSMPPLPVTPTPATPPTPPGLTPRR607 pKa = 11.84QRR609 pKa = 11.84LKK611 pKa = 11.08RR612 pKa = 11.84LITQHH617 pKa = 6.71AGWSPADD624 pKa = 3.76PGFLSSDD631 pKa = 3.97DD632 pKa = 4.59DD633 pKa = 4.61ADD635 pKa = 3.76AAPFEE640 pKa = 5.24DD641 pKa = 4.08EE642 pKa = 4.83DD643 pKa = 5.56DD644 pKa = 4.23RR645 pKa = 11.84EE646 pKa = 4.09EE647 pKa = 4.05WVQPKK652 pKa = 9.92RR653 pKa = 11.84PKK655 pKa = 10.33RR656 pKa = 11.84PCPFLDD662 pKa = 4.08SEE664 pKa = 4.42AEE666 pKa = 3.86EE667 pKa = 5.17DD668 pKa = 3.97GEE670 pKa = 4.19EE671 pKa = 5.59DD672 pKa = 3.24SGEE675 pKa = 4.07WSDD678 pKa = 4.29SAWRR682 pKa = 11.84EE683 pKa = 3.7LGGWSEE689 pKa = 4.65GSWEE693 pKa = 4.55DD694 pKa = 4.85FNPSDD699 pKa = 4.9ADD701 pKa = 3.65TDD703 pKa = 4.28STCSLNLEE711 pKa = 4.41RR712 pKa = 11.84WDD714 pKa = 3.31EE715 pKa = 4.38TTPVTHH721 pKa = 7.05RR722 pKa = 11.84KK723 pKa = 9.42VEE725 pKa = 4.04NLCPVKK731 pKa = 10.47QSAPTHH737 pKa = 5.96LGVPASRR744 pKa = 11.84EE745 pKa = 4.28LFPSMTPGAPPALQLPRR762 pKa = 11.84LTQTTIAQFIRR773 pKa = 11.84APPPSPVHH781 pKa = 6.71SNEE784 pKa = 4.03DD785 pKa = 3.88LFPVDD790 pKa = 5.95DD791 pKa = 5.88DD792 pKa = 5.35DD793 pKa = 6.93DD794 pKa = 4.31PTGLIALRR802 pKa = 11.84EE803 pKa = 4.1MPGGSGYY810 pKa = 10.51IMDD813 pKa = 4.34EE814 pKa = 4.25KK815 pKa = 10.57EE816 pKa = 4.0GKK818 pKa = 9.84NFFWGFWGSWGTSHH832 pKa = 7.53

Molecular weight: 94.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2RW96|A0A1B2RW96_9POLY ORF2 OS=Sparus aurata polyomavirus 1 OX=1885927 PE=4 SV=1
MM1 pKa = 7.61LISVSSPGPGQQQPAQQEE19 pKa = 4.07PRR21 pKa = 11.84HH22 pKa = 5.73FRR24 pKa = 11.84TFSHH28 pKa = 7.34PISLRR33 pKa = 11.84NEE35 pKa = 3.5RR36 pKa = 11.84RR37 pKa = 11.84VCLTLSRR44 pKa = 11.84AGPNWWKK51 pKa = 10.64APQSFFFGLAPPQNQISWRR70 pKa = 11.84EE71 pKa = 3.88KK72 pKa = 10.64QKK74 pKa = 10.93LAWCQLFFYY83 pKa = 10.37SGHH86 pKa = 6.05TPGTYY91 pKa = 10.22PRR93 pKa = 11.84MLSAGRR99 pKa = 11.84RR100 pKa = 11.84VHH102 pKa = 7.12CKK104 pKa = 10.48GEE106 pKa = 4.3KK107 pKa = 10.15KK108 pKa = 10.62LGTVPKK114 pKa = 10.16KK115 pKa = 10.44KK116 pKa = 10.31FHH118 pKa = 6.23II119 pKa = 4.48

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2068

111

832

413.6

45.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.559 ± 2.445

1.354 ± 0.624

5.851 ± 1.101

6.576 ± 0.544

3.433 ± 0.922

8.462 ± 1.062

2.805 ± 0.478

3.191 ± 0.113

5.029 ± 0.777

7.108 ± 0.716

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.95 ± 0.691

2.756 ± 0.469

7.012 ± 1.58

4.255 ± 0.514

6.431 ± 1.453

6.915 ± 0.673

5.416 ± 0.547

6.867 ± 0.491

2.273 ± 0.148

2.756 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski