Amino acid dipepetide frequency for Actinomyces sp. CtC 72

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.951AlaAla: 20.951 ± 0.293	1.309AlaCys: 1.309 ± 0.05	8.9AlaAsp: 8.9 ± 0.141	7.422AlaGlu: 7.422 ± 0.138	3.249AlaPhe: 3.249 ± 0.07	12.373AlaGly: 12.373 ± 0.168	2.552AlaHis: 2.552 ± 0.072	4.913AlaIle: 4.913 ± 0.099	2.167AlaLys: 2.167 ± 0.068	13.658AlaLeu: 13.658 ± 0.179	2.917AlaMet: 2.917 ± 0.061	2.472AlaAsn: 2.472 ± 0.072	6.513AlaPro: 6.513 ± 0.127	4.226AlaGln: 4.226 ± 0.097	9.423AlaArg: 9.423 ± 0.166	7.161AlaSer: 7.161 ± 0.131	7.761AlaThr: 7.761 ± 0.128	11.927AlaVal: 11.927 ± 0.178	2.138AlaTrp: 2.138 ± 0.063	2.479AlaTyr: 2.479 ± 0.069	0.0AlaXaa: 0.0 ± 0.0
Cys	1.133CysAla: 1.133 ± 0.049	0.163CysCys: 0.163 ± 0.017	0.443CysAsp: 0.443 ± 0.025	0.422CysGlu: 0.422 ± 0.029	0.241CysPhe: 0.241 ± 0.021	0.913CysGly: 0.913 ± 0.04	0.165CysHis: 0.165 ± 0.021	0.284CysIle: 0.284 ± 0.019	0.118CysLys: 0.118 ± 0.014	0.815CysLeu: 0.815 ± 0.035	0.161CysMet: 0.161 ± 0.021	0.134CysAsn: 0.134 ± 0.017	0.484CysPro: 0.484 ± 0.033	0.251CysGln: 0.251 ± 0.017	0.634CysArg: 0.634 ± 0.033	0.539CysSer: 0.539 ± 0.028	0.595CysThr: 0.595 ± 0.034	0.711CysVal: 0.711 ± 0.036	0.177CysTrp: 0.177 ± 0.016	0.203CysTyr: 0.203 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	9.094AspAla: 9.094 ± 0.137	0.411AspCys: 0.411 ± 0.027	4.227AspAsp: 4.227 ± 0.108	3.645AspGlu: 3.645 ± 0.086	1.609AspPhe: 1.609 ± 0.053	6.193AspGly: 6.193 ± 0.116	1.275AspHis: 1.275 ± 0.049	2.143AspIle: 2.143 ± 0.065	0.983AspLys: 0.983 ± 0.044	5.968AspLeu: 5.968 ± 0.112	1.063AspMet: 1.063 ± 0.041	1.125AspAsn: 1.125 ± 0.051	4.026AspPro: 4.026 ± 0.08	1.544AspGln: 1.544 ± 0.049	3.951AspArg: 3.951 ± 0.076	3.206AspSer: 3.206 ± 0.08	3.401AspThr: 3.401 ± 0.079	5.386AspVal: 5.386 ± 0.108	1.005AspTrp: 1.005 ± 0.04	1.593AspTyr: 1.593 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	7.114GluAla: 7.114 ± 0.108	0.406GluCys: 0.406 ± 0.024	3.017GluAsp: 3.017 ± 0.075	3.356GluGlu: 3.356 ± 0.074	1.323GluPhe: 1.323 ± 0.048	3.733GluGly: 3.733 ± 0.085	1.427GluHis: 1.427 ± 0.05	2.432GluIle: 2.432 ± 0.068	1.008GluLys: 1.008 ± 0.043	6.306GluLeu: 6.306 ± 0.106	0.984GluMet: 0.984 ± 0.039	1.151GluAsn: 1.151 ± 0.036	2.973GluPro: 2.973 ± 0.07	2.424GluGln: 2.424 ± 0.06	4.791GluArg: 4.791 ± 0.102	2.711GluSer: 2.711 ± 0.071	2.693GluThr: 2.693 ± 0.058	4.598GluVal: 4.598 ± 0.099	0.718GluTrp: 0.718 ± 0.036	1.199GluTyr: 1.199 ± 0.044	0.0GluXaa: 0.0 ± 0.0
Phe	3.267PheAla: 3.267 ± 0.074	0.219PheCys: 0.219 ± 0.021	1.91PheAsp: 1.91 ± 0.053	1.358PheGlu: 1.358 ± 0.051	0.941PhePhe: 0.941 ± 0.045	2.575PheGly: 2.575 ± 0.066	0.543PheHis: 0.543 ± 0.032	1.295PheIle: 1.295 ± 0.056	0.555PheLys: 0.555 ± 0.033	2.584PheLeu: 2.584 ± 0.075	0.55PheMet: 0.55 ± 0.027	0.805PheAsn: 0.805 ± 0.034	1.243PhePro: 1.243 ± 0.048	0.734PheGln: 0.734 ± 0.032	1.483PheArg: 1.483 ± 0.052	1.656PheSer: 1.656 ± 0.054	2.023PheThr: 2.023 ± 0.061	2.175PheVal: 2.175 ± 0.068	0.451PheTrp: 0.451 ± 0.026	0.721PheTyr: 0.721 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	10.981GlyAla: 10.981 ± 0.167	0.831GlyCys: 0.831 ± 0.04	4.67GlyAsp: 4.67 ± 0.094	4.782GlyGlu: 4.782 ± 0.074	2.624GlyPhe: 2.624 ± 0.065	7.03GlyGly: 7.03 ± 0.142	1.83GlyHis: 1.83 ± 0.053	3.895GlyIle: 3.895 ± 0.078	1.939GlyLys: 1.939 ± 0.063	8.624GlyLeu: 8.624 ± 0.124	2.071GlyMet: 2.071 ± 0.063	1.806GlyAsn: 1.806 ± 0.058	3.919GlyPro: 3.919 ± 0.096	2.773GlyGln: 2.773 ± 0.074	6.848GlyArg: 6.848 ± 0.129	5.292GlySer: 5.292 ± 0.107	5.659GlyThr: 5.659 ± 0.109	7.823GlyVal: 7.823 ± 0.121	1.603GlyTrp: 1.603 ± 0.054	2.421GlyTyr: 2.421 ± 0.072	0.0GlyXaa: 0.0 ± 0.0
His	2.386HisAla: 2.386 ± 0.067	0.174HisCys: 0.174 ± 0.018	1.282HisAsp: 1.282 ± 0.047	1.079HisGlu: 1.079 ± 0.048	0.555HisPhe: 0.555 ± 0.029	1.899HisGly: 1.899 ± 0.059	0.563HisHis: 0.563 ± 0.032	0.722HisIle: 0.722 ± 0.036	0.273HisLys: 0.273 ± 0.023	2.207HisLeu: 2.207 ± 0.064	0.384HisMet: 0.384 ± 0.024	0.42HisAsn: 0.42 ± 0.03	1.459HisPro: 1.459 ± 0.043	0.535HisGln: 0.535 ± 0.029	1.777HisArg: 1.777 ± 0.063	0.962HisSer: 0.962 ± 0.038	1.09HisThr: 1.09 ± 0.041	1.654HisVal: 1.654 ± 0.05	0.336HisTrp: 0.336 ± 0.023	0.502HisTyr: 0.502 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.52IleAla: 5.52 ± 0.093	0.372IleCys: 0.372 ± 0.027	3.12IleAsp: 3.12 ± 0.075	2.29IleGlu: 2.29 ± 0.071	1.024IlePhe: 1.024 ± 0.043	3.848IleGly: 3.848 ± 0.087	0.692IleHis: 0.692 ± 0.03	1.875IleIle: 1.875 ± 0.066	0.801IleLys: 0.801 ± 0.04	3.428IleLeu: 3.428 ± 0.086	0.85IleMet: 0.85 ± 0.042	1.103IleAsn: 1.103 ± 0.045	2.404IlePro: 2.404 ± 0.064	0.956IleGln: 0.956 ± 0.039	2.397IleArg: 2.397 ± 0.062	2.21IleSer: 2.21 ± 0.06	3.009IleThr: 3.009 ± 0.079	3.617IleVal: 3.617 ± 0.089	0.567IleTrp: 0.567 ± 0.031	0.834IleTyr: 0.834 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	2.237LysAla: 2.237 ± 0.071	0.064LysCys: 0.064 ± 0.009	1.215LysAsp: 1.215 ± 0.043	1.036LysGlu: 1.036 ± 0.042	0.401LysPhe: 0.401 ± 0.028	1.304LysGly: 1.304 ± 0.051	0.416LysHis: 0.416 ± 0.024	0.762LysIle: 0.762 ± 0.038	0.564LysLys: 0.564 ± 0.036	1.446LysLeu: 1.446 ± 0.056	0.409LysMet: 0.409 ± 0.022	0.532LysAsn: 0.532 ± 0.033	0.945LysPro: 0.945 ± 0.045	0.726LysGln: 0.726 ± 0.033	1.454LysArg: 1.454 ± 0.051	0.961LysSer: 0.961 ± 0.041	1.247LysThr: 1.247 ± 0.051	1.536LysVal: 1.536 ± 0.055	0.209LysTrp: 0.209 ± 0.017	0.489LysTyr: 0.489 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	14.532LeuAla: 14.532 ± 0.201	0.821LeuCys: 0.821 ± 0.037	6.46LeuAsp: 6.46 ± 0.106	5.092LeuGlu: 5.092 ± 0.099	2.453LeuPhe: 2.453 ± 0.077	8.912LeuGly: 8.912 ± 0.114	1.945LeuHis: 1.945 ± 0.064	4.023LeuIle: 4.023 ± 0.084	1.71LeuLys: 1.71 ± 0.055	10.689LeuLeu: 10.689 ± 0.171	1.987LeuMet: 1.987 ± 0.061	2.186LeuAsn: 2.186 ± 0.064	5.736LeuPro: 5.736 ± 0.094	2.332LeuGln: 2.332 ± 0.066	7.229LeuArg: 7.229 ± 0.131	5.755LeuSer: 5.755 ± 0.107	6.935LeuThr: 6.935 ± 0.101	8.53LeuVal: 8.53 ± 0.117	1.274LeuTrp: 1.274 ± 0.051	1.742LeuTyr: 1.742 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.415MetAla: 2.415 ± 0.064	0.147MetCys: 0.147 ± 0.015	1.084MetAsp: 1.084 ± 0.043	0.865MetGlu: 0.865 ± 0.041	0.545MetPhe: 0.545 ± 0.032	1.537MetGly: 1.537 ± 0.059	0.376MetHis: 0.376 ± 0.021	0.892MetIle: 0.892 ± 0.04	0.436MetLys: 0.436 ± 0.025	2.118MetLeu: 2.118 ± 0.061	0.425MetMet: 0.425 ± 0.026	0.545MetAsn: 0.545 ± 0.033	1.362MetPro: 1.362 ± 0.054	0.575MetGln: 0.575 ± 0.029	1.629MetArg: 1.629 ± 0.051	1.67MetSer: 1.67 ± 0.05	1.756MetThr: 1.756 ± 0.051	1.678MetVal: 1.678 ± 0.05	0.253MetTrp: 0.253 ± 0.016	0.36MetTyr: 0.36 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.603AsnAla: 2.603 ± 0.06	0.173AsnCys: 0.173 ± 0.016	1.339AsnAsp: 1.339 ± 0.051	1.088AsnGlu: 1.088 ± 0.048	0.569AsnPhe: 0.569 ± 0.032	1.892AsnGly: 1.892 ± 0.062	0.471AsnHis: 0.471 ± 0.032	0.919AsnIle: 0.919 ± 0.041	0.404AsnLys: 0.404 ± 0.028	2.137AsnLeu: 2.137 ± 0.058	0.376AsnMet: 0.376 ± 0.023	0.614AsnAsn: 0.614 ± 0.036	1.483AsnPro: 1.483 ± 0.049	0.7AsnGln: 0.7 ± 0.039	1.486AsnArg: 1.486 ± 0.052	1.258AsnSer: 1.258 ± 0.049	1.4AsnThr: 1.4 ± 0.049	1.528AsnVal: 1.528 ± 0.046	0.368AsnTrp: 0.368 ± 0.027	0.646AsnTyr: 0.646 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	7.849ProAla: 7.849 ± 0.136	0.355ProCys: 0.355 ± 0.027	4.051ProAsp: 4.051 ± 0.097	3.805ProGlu: 3.805 ± 0.087	1.432ProPhe: 1.432 ± 0.048	5.3ProGly: 5.3 ± 0.113	1.023ProHis: 1.023 ± 0.041	1.883ProIle: 1.883 ± 0.054	0.914ProLys: 0.914 ± 0.044	4.79ProLeu: 4.79 ± 0.09	1.034ProMet: 1.034 ± 0.042	1.084ProAsn: 1.084 ± 0.04	2.527ProPro: 2.527 ± 0.076	1.716ProGln: 1.716 ± 0.055	3.502ProArg: 3.502 ± 0.077	3.08ProSer: 3.08 ± 0.077	3.824ProThr: 3.824 ± 0.082	4.82ProVal: 4.82 ± 0.103	0.975ProTrp: 0.975 ± 0.042	1.211ProTyr: 1.211 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	4.35GlnAla: 4.35 ± 0.103	0.193GlnCys: 0.193 ± 0.017	1.523GlnAsp: 1.523 ± 0.049	1.777GlnGlu: 1.777 ± 0.057	0.743GlnPhe: 0.743 ± 0.037	2.07GlnGly: 2.07 ± 0.064	0.631GlnHis: 0.631 ± 0.033	1.29GlnIle: 1.29 ± 0.051	0.432GlnLys: 0.432 ± 0.027	2.928GlnLeu: 2.928 ± 0.076	0.689GlnMet: 0.689 ± 0.031	0.583GlnAsn: 0.583 ± 0.032	1.643GlnPro: 1.643 ± 0.058	1.245GlnGln: 1.245 ± 0.044	2.361GlnArg: 2.361 ± 0.067	1.445GlnSer: 1.445 ± 0.049	1.637GlnThr: 1.637 ± 0.061	2.835GlnVal: 2.835 ± 0.066	0.491GlnTrp: 0.491 ± 0.028	0.695GlnTyr: 0.695 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	8.769ArgAla: 8.769 ± 0.155	0.609ArgCys: 0.609 ± 0.035	3.706ArgAsp: 3.706 ± 0.079	4.173ArgGlu: 4.173 ± 0.086	2.094ArgPhe: 2.094 ± 0.056	5.395ArgGly: 5.395 ± 0.098	1.792ArgHis: 1.792 ± 0.066	3.254ArgIle: 3.254 ± 0.082	1.386ArgLys: 1.386 ± 0.053	7.959ArgLeu: 7.959 ± 0.135	1.814ArgMet: 1.814 ± 0.057	1.36ArgAsn: 1.36 ± 0.051	4.267ArgPro: 4.267 ± 0.1	2.17ArgGln: 2.17 ± 0.057	7.633ArgArg: 7.633 ± 0.146	4.234ArgSer: 4.234 ± 0.086	4.472ArgThr: 4.472 ± 0.099	5.682ArgVal: 5.682 ± 0.101	1.312ArgTrp: 1.312 ± 0.048	1.684ArgTyr: 1.684 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	7.241SerAla: 7.241 ± 0.131	0.511SerCys: 0.511 ± 0.032	3.064SerAsp: 3.064 ± 0.08	2.621SerGlu: 2.621 ± 0.065	1.801SerPhe: 1.801 ± 0.044	5.932SerGly: 5.932 ± 0.11	0.972SerHis: 0.972 ± 0.035	2.129SerIle: 2.129 ± 0.062	1.055SerLys: 1.055 ± 0.045	5.3SerLeu: 5.3 ± 0.093	1.291SerMet: 1.291 ± 0.049	1.184SerAsn: 1.184 ± 0.054	3.343SerPro: 3.343 ± 0.078	1.712SerGln: 1.712 ± 0.06	4.143SerArg: 4.143 ± 0.08	3.791SerSer: 3.791 ± 0.096	4.053SerThr: 4.053 ± 0.089	4.392SerVal: 4.392 ± 0.082	1.09SerTrp: 1.09 ± 0.037	1.366SerTyr: 1.366 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	8.777ThrAla: 8.777 ± 0.155	0.54ThrCys: 0.54 ± 0.033	3.86ThrAsp: 3.86 ± 0.075	2.973ThrGlu: 2.973 ± 0.075	1.697ThrPhe: 1.697 ± 0.055	6.118ThrGly: 6.118 ± 0.115	1.207ThrHis: 1.207 ± 0.044	2.898ThrIle: 2.898 ± 0.069	0.994ThrLys: 0.994 ± 0.044	6.057ThrLeu: 6.057 ± 0.109	1.261ThrMet: 1.261 ± 0.047	1.4ThrAsn: 1.4 ± 0.053	4.018ThrPro: 4.018 ± 0.07	1.622ThrGln: 1.622 ± 0.052	4.077ThrArg: 4.077 ± 0.078	3.724ThrSer: 3.724 ± 0.092	4.563ThrThr: 4.563 ± 0.108	5.992ThrVal: 5.992 ± 0.105	1.181ThrTrp: 1.181 ± 0.05	1.472ThrTyr: 1.472 ± 0.059	0.0ThrXaa: 0.0 ± 0.0
Val	10.917ValAla: 10.917 ± 0.147	0.882ValCys: 0.882 ± 0.04	5.487ValAsp: 5.487 ± 0.099	4.486ValGlu: 4.486 ± 0.082	2.571ValPhe: 2.571 ± 0.071	7.109ValGly: 7.109 ± 0.11	1.608ValHis: 1.608 ± 0.058	3.778ValIle: 3.778 ± 0.084	1.501ValLys: 1.501 ± 0.058	9.285ValLeu: 9.285 ± 0.158	1.688ValMet: 1.688 ± 0.055	1.827ValAsn: 1.827 ± 0.052	4.962ValPro: 4.962 ± 0.095	2.033ValGln: 2.033 ± 0.056	5.829ValArg: 5.829 ± 0.113	5.164ValSer: 5.164 ± 0.098	5.789ValThr: 5.789 ± 0.114	8.581ValVal: 8.581 ± 0.139	1.127ValTrp: 1.127 ± 0.038	1.659ValTyr: 1.659 ± 0.06	0.0ValXaa: 0.0 ± 0.0
Trp	1.716TrpAla: 1.716 ± 0.051	0.213TrpCys: 0.213 ± 0.019	1.002TrpAsp: 1.002 ± 0.047	0.829TrpGlu: 0.829 ± 0.041	0.499TrpPhe: 0.499 ± 0.033	1.155TrpGly: 1.155 ± 0.046	0.331TrpHis: 0.331 ± 0.025	0.722TrpIle: 0.722 ± 0.034	0.324TrpLys: 0.324 ± 0.021	1.704TrpLeu: 1.704 ± 0.054	0.361TrpMet: 0.361 ± 0.023	0.523TrpAsn: 0.523 ± 0.029	0.721TrpPro: 0.721 ± 0.035	0.539TrpGln: 0.539 ± 0.036	1.408TrpArg: 1.408 ± 0.056	0.97TrpSer: 0.97 ± 0.038	1.082TrpThr: 1.082 ± 0.041	1.14TrpVal: 1.14 ± 0.043	0.404TrpTrp: 0.404 ± 0.029	0.411TrpTyr: 0.411 ± 0.032	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.658TyrAla: 2.658 ± 0.079	0.193TyrCys: 0.193 ± 0.02	1.49TyrAsp: 1.49 ± 0.054	1.266TyrGlu: 1.266 ± 0.05	0.714TyrPhe: 0.714 ± 0.037	2.132TyrGly: 2.132 ± 0.064	0.441TyrHis: 0.441 ± 0.026	0.81TyrIle: 0.81 ± 0.043	0.388TyrLys: 0.388 ± 0.023	2.338TyrLeu: 2.338 ± 0.061	0.35TyrMet: 0.35 ± 0.022	0.599TyrAsn: 0.599 ± 0.032	1.148TyrPro: 1.148 ± 0.049	0.748TyrGln: 0.748 ± 0.038	1.71TyrArg: 1.71 ± 0.057	1.205TyrSer: 1.205 ± 0.05	1.443TyrThr: 1.443 ± 0.046	1.678TyrVal: 1.678 ± 0.056	0.376TyrTrp: 0.376 ± 0.022	0.644TyrTyr: 0.644 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2185 proteins (625708 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---