Parapedobacter composti
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3985 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I1M892|A0A1I1M892_9SPHI Uncharacterized protein OS=Parapedobacter composti OX=623281 GN=SAMN05421747_13015 PE=4 SV=1
MM1 pKa = 7.51 NFQSLLPIFPAIAVALLFTACEE23 pKa = 4.0 KK24 pKa = 11.04 VVDD27 pKa = 4.12 IDD29 pKa = 5.37 LDD31 pKa = 3.82 TAEE34 pKa = 4.6 PRR36 pKa = 11.84 LVIDD40 pKa = 5.51 AAISWEE46 pKa = 4.04 KK47 pKa = 9.87 GTDD50 pKa = 3.41 GANQRR55 pKa = 11.84 IRR57 pKa = 11.84 LSTTTDD63 pKa = 3.32 YY64 pKa = 11.35 YY65 pKa = 10.79 ATEE68 pKa = 4.22 IPPVSGATVQISNAAGQRR86 pKa = 11.84 FDD88 pKa = 4.53 FVEE91 pKa = 4.14 NPGTGEE97 pKa = 4.01 YY98 pKa = 10.44 LCTDD102 pKa = 4.58 FVPEE106 pKa = 3.65 LHH108 pKa = 6.4 GVYY111 pKa = 9.98 EE112 pKa = 4.14 LRR114 pKa = 11.84 VTVGDD119 pKa = 3.62 QVYY122 pKa = 7.94 TATEE126 pKa = 3.86 TLMPVPEE133 pKa = 4.05 ITRR136 pKa = 11.84 IEE138 pKa = 4.07 QRR140 pKa = 11.84 VLDD143 pKa = 3.71 IFGDD147 pKa = 3.83 NIEE150 pKa = 3.94 VRR152 pKa = 11.84 FYY154 pKa = 11.39 FNDD157 pKa = 3.91 DD158 pKa = 3.19 GTAEE162 pKa = 4.02 NYY164 pKa = 10.39 YY165 pKa = 9.27 LTEE168 pKa = 3.96 IQTDD172 pKa = 4.19 FLLFPSYY179 pKa = 11.31 GLLDD183 pKa = 3.62 NQYY186 pKa = 8.09 TQGNEE191 pKa = 3.6 LFQPFIDD198 pKa = 4.33 EE199 pKa = 4.41 EE200 pKa = 4.52 LDD202 pKa = 3.56 SGGEE206 pKa = 3.67 IQMRR210 pKa = 11.84 LSGISRR216 pKa = 11.84 QFYY219 pKa = 10.89 SFMALILAATGGNPFSTPPANIRR242 pKa = 11.84 GNIVNQTDD250 pKa = 3.37 AGNVALGYY258 pKa = 10.25 FRR260 pKa = 11.84 LSEE263 pKa = 4.43 TDD265 pKa = 2.89 TVTYY269 pKa = 10.5 VVEE272 pKa = 4.16
Molecular weight: 30.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.948
IPC_protein 3.923
Toseland 3.732
ProMoST 4.05
Dawson 3.897
Bjellqvist 4.075
Wikipedia 3.795
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.202
Thurlkill 3.77
EMBOSS 3.808
Sillero 4.037
Patrickios 1.901
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.947
Protein with the highest isoelectric point:
>tr|A0A1I1LVM5|A0A1I1LVM5_9SPHI Uncharacterized protein OS=Parapedobacter composti OX=623281 GN=SAMN05421747_12422 PE=4 SV=1
MM1 pKa = 7.49 ILKK4 pKa = 9.26 GCPKK8 pKa = 10.18 GSPIVKK14 pKa = 10.12 FEE16 pKa = 4.25 IANCAYY22 pKa = 9.72 PLSKK26 pKa = 10.5 RR27 pKa = 11.84 FNLSSNSAMYY37 pKa = 9.09 FRR39 pKa = 11.84 CRR41 pKa = 11.84 DD42 pKa = 3.35 IISPFLLTSGTRR54 pKa = 11.84 PDD56 pKa = 3.6 TYY58 pKa = 10.92 KK59 pKa = 10.84 SRR61 pKa = 11.84 SSWAALTVLSRR72 pKa = 11.84 FRR74 pKa = 11.84 YY75 pKa = 8.91 LGRR78 pKa = 11.84 IFRR81 pKa = 4.47
Molecular weight: 9.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.897
IPC_protein 10.672
Toseland 10.628
ProMoST 10.628
Dawson 10.76
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 11.008
Grimsley 10.833
Solomon 10.847
Lehninger 10.818
Nozaki 10.643
DTASelect 10.482
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.687
Patrickios 10.76
IPC_peptide 10.847
IPC2_peptide 9.692
IPC2.peptide.svr19 8.413
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3985
0
3985
1421768
24
3900
356.8
39.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.472 ± 0.041
0.745 ± 0.01
5.577 ± 0.025
5.843 ± 0.036
4.57 ± 0.024
7.535 ± 0.035
2.2 ± 0.022
6.233 ± 0.037
4.965 ± 0.043
9.418 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.277 ± 0.019
4.745 ± 0.032
4.22 ± 0.023
3.794 ± 0.022
5.62 ± 0.029
5.76 ± 0.027
5.643 ± 0.036
6.855 ± 0.033
1.353 ± 0.018
4.175 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here