Parapedobacter composti 
Average proteome isoelectric point is 6.57 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 3985 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A1I1M892|A0A1I1M892_9SPHI Uncharacterized protein OS=Parapedobacter composti OX=623281 GN=SAMN05421747_13015 PE=4 SV=1 
MM1 pKa = 7.51  NFQSLLPIFPAIAVALLFTACEE23 pKa = 4.0  KK24 pKa = 11.04  VVDD27 pKa = 4.12  IDD29 pKa = 5.37  LDD31 pKa = 3.82  TAEE34 pKa = 4.6  PRR36 pKa = 11.84  LVIDD40 pKa = 5.51  AAISWEE46 pKa = 4.04  KK47 pKa = 9.87  GTDD50 pKa = 3.41  GANQRR55 pKa = 11.84  IRR57 pKa = 11.84  LSTTTDD63 pKa = 3.32  YY64 pKa = 11.35  YY65 pKa = 10.79  ATEE68 pKa = 4.22  IPPVSGATVQISNAAGQRR86 pKa = 11.84  FDD88 pKa = 4.53  FVEE91 pKa = 4.14  NPGTGEE97 pKa = 4.01  YY98 pKa = 10.44  LCTDD102 pKa = 4.58  FVPEE106 pKa = 3.65  LHH108 pKa = 6.4  GVYY111 pKa = 9.98  EE112 pKa = 4.14  LRR114 pKa = 11.84  VTVGDD119 pKa = 3.62  QVYY122 pKa = 7.94  TATEE126 pKa = 3.86  TLMPVPEE133 pKa = 4.05  ITRR136 pKa = 11.84  IEE138 pKa = 4.07  QRR140 pKa = 11.84  VLDD143 pKa = 3.71  IFGDD147 pKa = 3.83  NIEE150 pKa = 3.94  VRR152 pKa = 11.84  FYY154 pKa = 11.39  FNDD157 pKa = 3.91  DD158 pKa = 3.19  GTAEE162 pKa = 4.02  NYY164 pKa = 10.39  YY165 pKa = 9.27  LTEE168 pKa = 3.96  IQTDD172 pKa = 4.19  FLLFPSYY179 pKa = 11.31  GLLDD183 pKa = 3.62  NQYY186 pKa = 8.09  TQGNEE191 pKa = 3.6  LFQPFIDD198 pKa = 4.33  EE199 pKa = 4.41  EE200 pKa = 4.52  LDD202 pKa = 3.56  SGGEE206 pKa = 3.67  IQMRR210 pKa = 11.84  LSGISRR216 pKa = 11.84  QFYY219 pKa = 10.89  SFMALILAATGGNPFSTPPANIRR242 pKa = 11.84  GNIVNQTDD250 pKa = 3.37  AGNVALGYY258 pKa = 10.25  FRR260 pKa = 11.84  LSEE263 pKa = 4.43  TDD265 pKa = 2.89  TVTYY269 pKa = 10.5  VVEE272 pKa = 4.16   
 Molecular weight: 30.2 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.775 
IPC2_protein 3.948 
IPC_protein 3.923 
Toseland    3.732 
ProMoST     4.05 
Dawson      3.897 
Bjellqvist  4.075 
Wikipedia   3.795 
Rodwell     3.757 
Grimsley    3.643 
Solomon     3.897 
Lehninger   3.846 
Nozaki      4.012 
DTASelect   4.202 
Thurlkill   3.77 
EMBOSS      3.808 
Sillero     4.037 
Patrickios  1.901 
IPC_peptide 3.897 
IPC2_peptide  4.024 
IPC2.peptide.svr19  3.947 
 Protein with the highest isoelectric point: 
>tr|A0A1I1LVM5|A0A1I1LVM5_9SPHI Uncharacterized protein OS=Parapedobacter composti OX=623281 GN=SAMN05421747_12422 PE=4 SV=1 
MM1 pKa = 7.49  ILKK4 pKa = 9.26  GCPKK8 pKa = 10.18  GSPIVKK14 pKa = 10.12  FEE16 pKa = 4.25  IANCAYY22 pKa = 9.72  PLSKK26 pKa = 10.5  RR27 pKa = 11.84  FNLSSNSAMYY37 pKa = 9.09  FRR39 pKa = 11.84  CRR41 pKa = 11.84  DD42 pKa = 3.35  IISPFLLTSGTRR54 pKa = 11.84  PDD56 pKa = 3.6  TYY58 pKa = 10.92  KK59 pKa = 10.84  SRR61 pKa = 11.84  SSWAALTVLSRR72 pKa = 11.84  FRR74 pKa = 11.84  YY75 pKa = 8.91  LGRR78 pKa = 11.84  IFRR81 pKa = 4.47   
 Molecular weight: 9.27 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.343 
IPC2_protein 9.897 
IPC_protein 10.672 
Toseland    10.628 
ProMoST     10.628 
Dawson      10.76 
Bjellqvist  10.496 
Wikipedia   10.979 
Rodwell     11.008 
Grimsley    10.833 
Solomon     10.847 
Lehninger   10.818 
Nozaki      10.643 
DTASelect   10.482 
Thurlkill   10.643 
EMBOSS      11.023 
Sillero     10.687 
Patrickios  10.76 
IPC_peptide 10.847 
IPC2_peptide  9.692 
IPC2.peptide.svr19  8.413 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        3985 
 
        
        0
 
        
        3985 
         
        1421768
 
        24
 
        3900
 
        356.8
 
        39.93
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        8.472 ± 0.041
0.745 ± 0.01
 
        5.577 ± 0.025
5.843 ± 0.036
 
        4.57 ± 0.024
7.535 ± 0.035
 
        2.2 ± 0.022
6.233 ± 0.037
       
        4.965 ± 0.043
9.418 ± 0.039
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.277 ± 0.019
4.745 ± 0.032
 
        4.22 ± 0.023
3.794 ± 0.022
 
        5.62 ± 0.029
5.76 ± 0.027
 
        5.643 ± 0.036
6.855 ± 0.033
       
        1.353 ± 0.018
4.175 ± 0.028
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here