Gokushovirinae GNX3R
Average proteome isoelectric point is 7.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M4MQJ0|A0A0M4MQJ0_9VIRU Putative major capsid protein OS=Gokushovirinae GNX3R OX=1701405 PE=3 SV=1
MM1 pKa = 7.4 HH2 pKa = 7.53 RR3 pKa = 11.84 NKK5 pKa = 10.55 SVDD8 pKa = 3.11 VHH10 pKa = 6.0 QFTMIPKK17 pKa = 9.68 ADD19 pKa = 3.47 IPRR22 pKa = 11.84 SSFDD26 pKa = 3.38 CQSTHH31 pKa = 5.3 KK32 pKa = 8.11 TTFDD36 pKa = 3.09 AGFLVPVYY44 pKa = 10.21 VDD46 pKa = 3.42 EE47 pKa = 4.67 MLPGDD52 pKa = 4.12 TFRR55 pKa = 11.84 LNMTAFARR63 pKa = 11.84 LATPLYY69 pKa = 9.79 PIMDD73 pKa = 4.06 NMHH76 pKa = 7.03 LDD78 pKa = 3.37 SFFFFVPNRR87 pKa = 11.84 LIWNNWQKK95 pKa = 11.19 FMGQQANPDD104 pKa = 3.42 SSISFVVPQQVSPAGGYY121 pKa = 10.7 AIGSLQDD128 pKa = 3.62 YY129 pKa = 9.06 MGLPTVGQVGAGNTVSHH146 pKa = 6.57 CAFWPRR152 pKa = 11.84 AYY154 pKa = 11.08 NLIYY158 pKa = 10.8 NEE160 pKa = 3.89 WFRR163 pKa = 11.84 DD164 pKa = 3.51 EE165 pKa = 4.86 NLQNSVTVDD174 pKa = 3.41 LGDD177 pKa = 4.63 GPDD180 pKa = 3.16 TVTNYY185 pKa = 9.58 TLLRR189 pKa = 11.84 RR190 pKa = 11.84 GKK192 pKa = 9.86 RR193 pKa = 11.84 KK194 pKa = 10.37 DD195 pKa = 3.57 YY196 pKa = 8.24 FTSALPWPQKK206 pKa = 8.94 GTSVTLPLGTSAPVYY221 pKa = 9.24 GTGRR225 pKa = 11.84 TIGVSNGSGNFGLTTNSAGGVFGYY249 pKa = 10.87 AGAYY253 pKa = 9.48 DD254 pKa = 3.72 KK255 pKa = 11.52 ALGGSNITGTILATKK270 pKa = 9.92 DD271 pKa = 2.95 IGVVTSGVSGLYY283 pKa = 10.39 ADD285 pKa = 5.29 LSVATAATINQLRR298 pKa = 11.84 QSFQIQKK305 pKa = 10.32 LLEE308 pKa = 4.31 RR309 pKa = 11.84 DD310 pKa = 3.28 ARR312 pKa = 11.84 GGTRR316 pKa = 11.84 YY317 pKa = 8.58 TEE319 pKa = 4.72 IIRR322 pKa = 11.84 SHH324 pKa = 6.81 FGVISPDD331 pKa = 3.07 ARR333 pKa = 11.84 LQRR336 pKa = 11.84 PEE338 pKa = 4.12 YY339 pKa = 10.25 IGGGSTNININPIAQTSGSNASGTTTPMGTLAAMGTALAHH379 pKa = 5.36 NHH381 pKa = 5.54 GFTYY385 pKa = 10.46 SATEE389 pKa = 3.7 HH390 pKa = 6.31 GVILGLVSVRR400 pKa = 11.84 ADD402 pKa = 3.2 LTYY405 pKa = 10.57 QQGLQRR411 pKa = 11.84 MWSRR415 pKa = 11.84 STRR418 pKa = 11.84 YY419 pKa = 10.23 DD420 pKa = 3.49 FYY422 pKa = 11.49 FPAFATLGEE431 pKa = 3.95 QAILNKK437 pKa = 9.91 EE438 pKa = 3.96 IYY440 pKa = 8.76 VTGSSGDD447 pKa = 3.4 NDD449 pKa = 3.28 VFGYY453 pKa = 7.2 QEE455 pKa = 3.24 RR456 pKa = 11.84 WAEE459 pKa = 3.71 YY460 pKa = 9.05 RR461 pKa = 11.84 YY462 pKa = 9.04 YY463 pKa = 10.13 PSRR466 pKa = 11.84 ISSLFRR472 pKa = 11.84 STAAGTIDD480 pKa = 3.63 AWHH483 pKa = 7.2 LAQKK487 pKa = 8.59 FTATPTLNTTFIQDD501 pKa = 3.74 TPPVSRR507 pKa = 11.84 VVAVGAAANGQQFIFDD523 pKa = 4.19 SFFDD527 pKa = 4.02 CKK529 pKa = 10.24 KK530 pKa = 10.03 ARR532 pKa = 11.84 PMPMYY537 pKa = 10.18 SVPGLIDD544 pKa = 3.33 HH545 pKa = 7.34 FYY547 pKa = 8.73 MLGGISLGGVTGGLLGFIGQQQTNQNNXGYY577 pKa = 9.99 SSSRR581 pKa = 3.34
Molecular weight: 63.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.595
IPC2_protein 7.849
IPC_protein 7.892
Toseland 7.849
ProMoST 8.375
Dawson 8.434
Bjellqvist 8.521
Wikipedia 8.478
Rodwell 8.434
Grimsley 7.892
Solomon 8.595
Lehninger 8.565
Nozaki 8.463
DTASelect 8.419
Thurlkill 8.434
EMBOSS 8.609
Sillero 8.653
Patrickios 3.961
IPC_peptide 8.58
IPC2_peptide 7.556
IPC2.peptide.svr19 7.698
Protein with the highest isoelectric point:
>tr|A0A0M4MQJ0|A0A0M4MQJ0_9VIRU Putative major capsid protein OS=Gokushovirinae GNX3R OX=1701405 PE=3 SV=1
MM1 pKa = 7.4 HH2 pKa = 7.53 RR3 pKa = 11.84 NKK5 pKa = 10.55 SVDD8 pKa = 3.11 VHH10 pKa = 6.0 QFTMIPKK17 pKa = 9.68 ADD19 pKa = 3.47 IPRR22 pKa = 11.84 SSFDD26 pKa = 3.38 CQSTHH31 pKa = 5.3 KK32 pKa = 8.11 TTFDD36 pKa = 3.09 AGFLVPVYY44 pKa = 10.21 VDD46 pKa = 3.42 EE47 pKa = 4.67 MLPGDD52 pKa = 4.12 TFRR55 pKa = 11.84 LNMTAFARR63 pKa = 11.84 LATPLYY69 pKa = 9.79 PIMDD73 pKa = 4.06 NMHH76 pKa = 7.03 LDD78 pKa = 3.37 SFFFFVPNRR87 pKa = 11.84 LIWNNWQKK95 pKa = 11.19 FMGQQANPDD104 pKa = 3.42 SSISFVVPQQVSPAGGYY121 pKa = 10.7 AIGSLQDD128 pKa = 3.62 YY129 pKa = 9.06 MGLPTVGQVGAGNTVSHH146 pKa = 6.57 CAFWPRR152 pKa = 11.84 AYY154 pKa = 11.08 NLIYY158 pKa = 10.8 NEE160 pKa = 3.89 WFRR163 pKa = 11.84 DD164 pKa = 3.51 EE165 pKa = 4.86 NLQNSVTVDD174 pKa = 3.41 LGDD177 pKa = 4.63 GPDD180 pKa = 3.16 TVTNYY185 pKa = 9.58 TLLRR189 pKa = 11.84 RR190 pKa = 11.84 GKK192 pKa = 9.86 RR193 pKa = 11.84 KK194 pKa = 10.37 DD195 pKa = 3.57 YY196 pKa = 8.24 FTSALPWPQKK206 pKa = 8.94 GTSVTLPLGTSAPVYY221 pKa = 9.24 GTGRR225 pKa = 11.84 TIGVSNGSGNFGLTTNSAGGVFGYY249 pKa = 10.87 AGAYY253 pKa = 9.48 DD254 pKa = 3.72 KK255 pKa = 11.52 ALGGSNITGTILATKK270 pKa = 9.92 DD271 pKa = 2.95 IGVVTSGVSGLYY283 pKa = 10.39 ADD285 pKa = 5.29 LSVATAATINQLRR298 pKa = 11.84 QSFQIQKK305 pKa = 10.32 LLEE308 pKa = 4.31 RR309 pKa = 11.84 DD310 pKa = 3.28 ARR312 pKa = 11.84 GGTRR316 pKa = 11.84 YY317 pKa = 8.58 TEE319 pKa = 4.72 IIRR322 pKa = 11.84 SHH324 pKa = 6.81 FGVISPDD331 pKa = 3.07 ARR333 pKa = 11.84 LQRR336 pKa = 11.84 PEE338 pKa = 4.12 YY339 pKa = 10.25 IGGGSTNININPIAQTSGSNASGTTTPMGTLAAMGTALAHH379 pKa = 5.36 NHH381 pKa = 5.54 GFTYY385 pKa = 10.46 SATEE389 pKa = 3.7 HH390 pKa = 6.31 GVILGLVSVRR400 pKa = 11.84 ADD402 pKa = 3.2 LTYY405 pKa = 10.57 QQGLQRR411 pKa = 11.84 MWSRR415 pKa = 11.84 STRR418 pKa = 11.84 YY419 pKa = 10.23 DD420 pKa = 3.49 FYY422 pKa = 11.49 FPAFATLGEE431 pKa = 3.95 QAILNKK437 pKa = 9.91 EE438 pKa = 3.96 IYY440 pKa = 8.76 VTGSSGDD447 pKa = 3.4 NDD449 pKa = 3.28 VFGYY453 pKa = 7.2 QEE455 pKa = 3.24 RR456 pKa = 11.84 WAEE459 pKa = 3.71 YY460 pKa = 9.05 RR461 pKa = 11.84 YY462 pKa = 9.04 YY463 pKa = 10.13 PSRR466 pKa = 11.84 ISSLFRR472 pKa = 11.84 STAAGTIDD480 pKa = 3.63 AWHH483 pKa = 7.2 LAQKK487 pKa = 8.59 FTATPTLNTTFIQDD501 pKa = 3.74 TPPVSRR507 pKa = 11.84 VVAVGAAANGQQFIFDD523 pKa = 4.19 SFFDD527 pKa = 4.02 CKK529 pKa = 10.24 KK530 pKa = 10.03 ARR532 pKa = 11.84 PMPMYY537 pKa = 10.18 SVPGLIDD544 pKa = 3.33 HH545 pKa = 7.34 FYY547 pKa = 8.73 MLGGISLGGVTGGLLGFIGQQQTNQNNXGYY577 pKa = 9.99 SSSRR581 pKa = 3.34
Molecular weight: 63.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.595
IPC2_protein 7.849
IPC_protein 7.892
Toseland 7.849
ProMoST 8.375
Dawson 8.434
Bjellqvist 8.521
Wikipedia 8.478
Rodwell 8.434
Grimsley 7.892
Solomon 8.595
Lehninger 8.565
Nozaki 8.463
DTASelect 8.419
Thurlkill 8.434
EMBOSS 8.609
Sillero 8.653
Patrickios 3.961
IPC_peptide 8.58
IPC2_peptide 7.556
IPC2.peptide.svr19 7.698
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
581
581
581
581.0
63.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.917 ± 0.0
0.516 ± 0.0
4.991 ± 0.0
1.893 ± 0.0
5.68 ± 0.0
10.671 ± 0.0
1.893 ± 0.0
5.164 ± 0.0
2.41 ± 0.0
7.229 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.41 ± 0.0
5.164 ± 0.0
4.991 ± 0.0
5.164 ± 0.0
4.991 ± 0.0
7.917 ± 0.0
8.95 ± 0.0
6.024 ± 0.0
1.377 ± 0.0
4.475 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here