Amino acid dipepetide frequency for Lactococcus phage 936 group phage PhiF0139

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.539AlaAla: 0.539 ± 0.262
0.108AlaCys: 0.108 ± 0.102
3.018AlaAsp: 3.018 ± 0.575
4.527AlaGlu: 4.527 ± 0.81
3.665AlaPhe: 3.665 ± 0.821
3.988AlaGly: 3.988 ± 0.67
0.754AlaHis: 0.754 ± 0.279
4.419AlaIle: 4.419 ± 0.907
5.712AlaLys: 5.712 ± 0.923
6.359AlaLeu: 6.359 ± 1.28
2.479AlaMet: 2.479 ± 0.701
4.527AlaAsn: 4.527 ± 1.05
0.862AlaPro: 0.862 ± 0.362
2.263AlaGln: 2.263 ± 0.483
2.479AlaArg: 2.479 ± 0.566
3.126AlaSer: 3.126 ± 0.828
3.88AlaThr: 3.88 ± 0.792
3.88AlaVal: 3.88 ± 0.878
1.617AlaTrp: 1.617 ± 0.736
1.832AlaTyr: 1.832 ± 0.367
0.0AlaXaa: 0.0 ± 0.0
Cys
0.323CysAla: 0.323 ± 0.169
0.0CysCys: 0.0 ± 0.0
0.431CysAsp: 0.431 ± 0.242
0.431CysGlu: 0.431 ± 0.194
0.216CysPhe: 0.216 ± 0.163
1.078CysGly: 1.078 ± 0.358
0.431CysHis: 0.431 ± 0.262
0.539CysIle: 0.539 ± 0.246
0.862CysLys: 0.862 ± 0.338
0.216CysLeu: 0.216 ± 0.164
0.108CysMet: 0.108 ± 0.109
0.539CysAsn: 0.539 ± 0.264
0.108CysPro: 0.108 ± 0.11
0.216CysGln: 0.216 ± 0.137
0.647CysArg: 0.647 ± 0.277
0.323CysSer: 0.323 ± 0.199
0.108CysThr: 0.108 ± 0.121
0.323CysVal: 0.323 ± 0.191
0.108CysTrp: 0.108 ± 0.108
0.431CysTyr: 0.431 ± 0.247
0.0CysXaa: 0.0 ± 0.0
Asp
2.479AspAla: 2.479 ± 0.708
0.216AspCys: 0.216 ± 0.143
3.126AspAsp: 3.126 ± 0.682
4.527AspGlu: 4.527 ± 0.936
3.988AspPhe: 3.988 ± 0.544
3.341AspGly: 3.341 ± 0.621
0.862AspHis: 0.862 ± 0.321
4.096AspIle: 4.096 ± 0.698
5.605AspLys: 5.605 ± 0.797
6.359AspLeu: 6.359 ± 0.922
0.97AspMet: 0.97 ± 0.275
4.635AspAsn: 4.635 ± 0.73
1.293AspPro: 1.293 ± 0.373
0.97AspGln: 0.97 ± 0.26
1.832AspArg: 1.832 ± 0.521
3.341AspSer: 3.341 ± 0.553
3.88AspThr: 3.88 ± 0.72
2.587AspVal: 2.587 ± 0.513
1.078AspTrp: 1.078 ± 0.313
3.126AspTyr: 3.126 ± 0.635
0.0AspXaa: 0.0 ± 0.0
Glu
3.988GluAla: 3.988 ± 0.689
0.539GluCys: 0.539 ± 0.276
2.695GluAsp: 2.695 ± 0.573
6.144GluGlu: 6.144 ± 1.032
3.341GluPhe: 3.341 ± 0.654
2.802GluGly: 2.802 ± 0.479
0.754GluHis: 0.754 ± 0.358
7.006GluIle: 7.006 ± 1.07
6.575GluLys: 6.575 ± 1.134
10.455GluLeu: 10.455 ± 1.277
2.048GluMet: 2.048 ± 0.429
4.419GluAsn: 4.419 ± 0.554
1.401GluPro: 1.401 ± 0.431
4.311GluGln: 4.311 ± 0.685
2.91GluArg: 2.91 ± 0.579
3.449GluSer: 3.449 ± 0.555
5.389GluThr: 5.389 ± 0.634
4.85GluVal: 4.85 ± 0.754
0.97GluTrp: 0.97 ± 0.326
3.233GluTyr: 3.233 ± 0.789
0.0GluXaa: 0.0 ± 0.0
Phe
2.479PheAla: 2.479 ± 0.664
0.323PheCys: 0.323 ± 0.188
3.88PheAsp: 3.88 ± 0.585
2.91PheGlu: 2.91 ± 0.635
1.617PhePhe: 1.617 ± 0.631
2.479PheGly: 2.479 ± 0.469
0.216PheHis: 0.216 ± 0.172
3.449PheIle: 3.449 ± 0.646
3.665PheLys: 3.665 ± 0.668
2.587PheLeu: 2.587 ± 0.543
1.186PheMet: 1.186 ± 0.321
2.802PheAsn: 2.802 ± 0.682
0.862PhePro: 0.862 ± 0.251
1.293PheGln: 1.293 ± 0.321
1.401PheArg: 1.401 ± 0.293
3.988PheSer: 3.988 ± 0.66
2.91PheThr: 2.91 ± 0.49
2.263PheVal: 2.263 ± 0.453
0.216PheTrp: 0.216 ± 0.15
1.509PheTyr: 1.509 ± 0.416
0.0PheXaa: 0.0 ± 0.0
Gly
3.341GlyAla: 3.341 ± 1.104
0.323GlyCys: 0.323 ± 0.163
3.233GlyAsp: 3.233 ± 0.601
3.988GlyGlu: 3.988 ± 0.711
1.617GlyPhe: 1.617 ± 0.374
4.203GlyGly: 4.203 ± 0.925
0.754GlyHis: 0.754 ± 0.265
3.772GlyIle: 3.772 ± 0.955
6.251GlyLys: 6.251 ± 0.711
5.497GlyLeu: 5.497 ± 1.153
1.078GlyMet: 1.078 ± 0.332
3.018GlyAsn: 3.018 ± 0.586
0.0GlyPro: 0.0 ± 0.0
1.94GlyGln: 1.94 ± 0.513
2.263GlyArg: 2.263 ± 0.439
4.527GlySer: 4.527 ± 0.872
3.988GlyThr: 3.988 ± 0.985
5.497GlyVal: 5.497 ± 1.266
1.293GlyTrp: 1.293 ± 0.34
3.449GlyTyr: 3.449 ± 0.63
0.0GlyXaa: 0.0 ± 0.0
His
0.862HisAla: 0.862 ± 0.259
0.647HisCys: 0.647 ± 0.296
0.754HisAsp: 0.754 ± 0.314
0.647HisGlu: 0.647 ± 0.255
0.539HisPhe: 0.539 ± 0.237
1.078HisGly: 1.078 ± 0.404
0.0HisHis: 0.0 ± 0.0
0.97HisIle: 0.97 ± 0.314
0.862HisLys: 0.862 ± 0.288
0.862HisLeu: 0.862 ± 0.341
0.0HisMet: 0.0 ± 0.0
1.509HisAsn: 1.509 ± 0.415
0.216HisPro: 0.216 ± 0.149
0.323HisGln: 0.323 ± 0.202
0.323HisArg: 0.323 ± 0.199
0.323HisSer: 0.323 ± 0.151
1.078HisThr: 1.078 ± 0.387
0.647HisVal: 0.647 ± 0.242
0.216HisTrp: 0.216 ± 0.143
0.862HisTyr: 0.862 ± 0.31
0.0HisXaa: 0.0 ± 0.0
Ile
4.742IleAla: 4.742 ± 0.723
0.108IleCys: 0.108 ± 0.108
4.419IleAsp: 4.419 ± 0.671
6.467IleGlu: 6.467 ± 0.838
2.587IlePhe: 2.587 ± 0.546
3.018IleGly: 3.018 ± 0.656
1.078IleHis: 1.078 ± 0.369
4.85IleIle: 4.85 ± 0.684
6.575IleLys: 6.575 ± 0.943
5.497IleLeu: 5.497 ± 0.974
1.186IleMet: 1.186 ± 0.375
5.928IleAsn: 5.928 ± 0.614
1.725IlePro: 1.725 ± 0.344
2.802IleGln: 2.802 ± 0.503
1.617IleArg: 1.617 ± 0.399
4.85IleSer: 4.85 ± 0.913
4.958IleThr: 4.958 ± 0.629
4.203IleVal: 4.203 ± 0.679
1.293IleTrp: 1.293 ± 0.458
2.156IleTyr: 2.156 ± 0.405
0.0IleXaa: 0.0 ± 0.0
Lys
7.114LysAla: 7.114 ± 1.037
1.078LysCys: 1.078 ± 0.445
5.82LysAsp: 5.82 ± 0.648
8.407LysGlu: 8.407 ± 1.479
2.587LysPhe: 2.587 ± 0.597
5.066LysGly: 5.066 ± 1.049
1.509LysHis: 1.509 ± 0.468
5.605LysIle: 5.605 ± 0.77
9.054LysLys: 9.054 ± 1.201
7.545LysLeu: 7.545 ± 0.729
3.557LysMet: 3.557 ± 0.5
4.742LysAsn: 4.742 ± 0.641
1.617LysPro: 1.617 ± 0.463
4.203LysGln: 4.203 ± 0.809
3.126LysArg: 3.126 ± 0.578
5.605LysSer: 5.605 ± 0.88
5.497LysThr: 5.497 ± 0.832
5.82LysVal: 5.82 ± 0.825
1.078LysTrp: 1.078 ± 0.298
4.096LysTyr: 4.096 ± 0.693
0.0LysXaa: 0.0 ± 0.0
Leu
5.389LeuAla: 5.389 ± 0.624
0.431LeuCys: 0.431 ± 0.235
5.066LeuAsp: 5.066 ± 0.703
6.251LeuGlu: 6.251 ± 0.831
3.665LeuPhe: 3.665 ± 0.622
4.527LeuGly: 4.527 ± 1.28
1.401LeuHis: 1.401 ± 0.361
6.359LeuIle: 6.359 ± 0.994
9.269LeuLys: 9.269 ± 1.087
6.682LeuLeu: 6.682 ± 1.249
1.725LeuMet: 1.725 ± 0.451
5.389LeuAsn: 5.389 ± 0.963
3.126LeuPro: 3.126 ± 0.598
3.018LeuGln: 3.018 ± 0.478
2.91LeuArg: 2.91 ± 0.51
4.742LeuSer: 4.742 ± 0.742
5.82LeuThr: 5.82 ± 0.711
5.605LeuVal: 5.605 ± 0.634
1.186LeuTrp: 1.186 ± 0.355
4.311LeuTyr: 4.311 ± 0.76
0.0LeuXaa: 0.0 ± 0.0
Met
2.048MetAla: 2.048 ± 0.529
0.216MetCys: 0.216 ± 0.161
1.293MetAsp: 1.293 ± 0.417
1.832MetGlu: 1.832 ± 0.53
0.323MetPhe: 0.323 ± 0.161
0.97MetGly: 0.97 ± 0.342
0.216MetHis: 0.216 ± 0.148
2.156MetIle: 2.156 ± 0.504
2.695MetLys: 2.695 ± 0.61
1.401MetLeu: 1.401 ± 0.395
0.323MetMet: 0.323 ± 0.204
1.509MetAsn: 1.509 ± 0.443
0.647MetPro: 0.647 ± 0.251
1.509MetGln: 1.509 ± 0.266
0.216MetArg: 0.216 ± 0.156
1.832MetSer: 1.832 ± 0.417
2.048MetThr: 2.048 ± 0.568
1.401MetVal: 1.401 ± 0.395
0.216MetTrp: 0.216 ± 0.128
1.293MetTyr: 1.293 ± 0.374
0.0MetXaa: 0.0 ± 0.0
Asn
4.85AsnAla: 4.85 ± 0.956
0.216AsnCys: 0.216 ± 0.172
4.635AsnAsp: 4.635 ± 0.806
4.635AsnGlu: 4.635 ± 0.884
2.587AsnPhe: 2.587 ± 0.562
6.359AsnGly: 6.359 ± 0.876
0.647AsnHis: 0.647 ± 0.284
4.85AsnIle: 4.85 ± 0.943
6.898AsnLys: 6.898 ± 1.305
5.281AsnLeu: 5.281 ± 0.822
1.725AsnMet: 1.725 ± 0.34
3.126AsnAsn: 3.126 ± 0.567
2.156AsnPro: 2.156 ± 0.509
2.371AsnGln: 2.371 ± 0.479
1.832AsnArg: 1.832 ± 0.396
4.419AsnSer: 4.419 ± 0.663
3.988AsnThr: 3.988 ± 0.953
3.233AsnVal: 3.233 ± 0.621
0.862AsnTrp: 0.862 ± 0.384
1.94AsnTyr: 1.94 ± 0.542
0.0AsnXaa: 0.0 ± 0.0
Pro
1.617ProAla: 1.617 ± 0.368
0.216ProCys: 0.216 ± 0.166
1.509ProAsp: 1.509 ± 0.428
1.617ProGlu: 1.617 ± 0.426
0.754ProPhe: 0.754 ± 0.257
0.323ProGly: 0.323 ± 0.172
0.108ProHis: 0.108 ± 0.119
2.263ProIle: 2.263 ± 0.618
2.802ProLys: 2.802 ± 0.646
1.617ProLeu: 1.617 ± 0.359
0.647ProMet: 0.647 ± 0.25
1.94ProAsn: 1.94 ± 0.671
0.539ProPro: 0.539 ± 0.234
0.539ProGln: 0.539 ± 0.291
0.754ProArg: 0.754 ± 0.276
0.754ProSer: 0.754 ± 0.313
2.048ProThr: 2.048 ± 0.394
1.401ProVal: 1.401 ± 0.376
0.0ProTrp: 0.0 ± 0.0
0.754ProTyr: 0.754 ± 0.33
0.0ProXaa: 0.0 ± 0.0
Gln
3.126GlnAla: 3.126 ± 0.762
0.216GlnCys: 0.216 ± 0.145
2.048GlnAsp: 2.048 ± 0.514
2.371GlnGlu: 2.371 ± 0.527
0.97GlnPhe: 0.97 ± 0.285
2.695GlnGly: 2.695 ± 0.516
0.431GlnHis: 0.431 ± 0.197
1.186GlnIle: 1.186 ± 0.246
3.018GlnLys: 3.018 ± 0.586
3.665GlnLeu: 3.665 ± 0.652
0.862GlnMet: 0.862 ± 0.283
2.156GlnAsn: 2.156 ± 0.328
1.186GlnPro: 1.186 ± 0.409
1.725GlnGln: 1.725 ± 0.496
1.509GlnArg: 1.509 ± 0.442
2.479GlnSer: 2.479 ± 0.46
2.371GlnThr: 2.371 ± 0.498
2.479GlnVal: 2.479 ± 0.503
0.647GlnTrp: 0.647 ± 0.215
1.186GlnTyr: 1.186 ± 0.312
0.0GlnXaa: 0.0 ± 0.0
Arg
1.94ArgAla: 1.94 ± 0.522
0.431ArgCys: 0.431 ± 0.212
1.617ArgAsp: 1.617 ± 0.386
2.695ArgGlu: 2.695 ± 0.55
0.97ArgPhe: 0.97 ± 0.306
2.048ArgGly: 2.048 ± 0.438
0.754ArgHis: 0.754 ± 0.295
1.832ArgIle: 1.832 ± 0.419
3.988ArgLys: 3.988 ± 0.716
3.772ArgLeu: 3.772 ± 0.751
0.539ArgMet: 0.539 ± 0.29
2.802ArgAsn: 2.802 ± 0.57
0.539ArgPro: 0.539 ± 0.242
1.186ArgGln: 1.186 ± 0.345
1.617ArgArg: 1.617 ± 0.433
1.617ArgSer: 1.617 ± 0.415
2.048ArgThr: 2.048 ± 0.439
1.94ArgVal: 1.94 ± 0.471
0.431ArgTrp: 0.431 ± 0.204
1.725ArgTyr: 1.725 ± 0.468
0.0ArgXaa: 0.0 ± 0.0
Ser
4.742SerAla: 4.742 ± 1.423
0.647SerCys: 0.647 ± 0.341
3.88SerAsp: 3.88 ± 0.643
4.419SerGlu: 4.419 ± 0.612
3.233SerPhe: 3.233 ± 0.596
4.85SerGly: 4.85 ± 1.071
0.862SerHis: 0.862 ± 0.321
4.85SerIle: 4.85 ± 0.702
5.066SerLys: 5.066 ± 0.732
5.174SerLeu: 5.174 ± 0.788
1.401SerMet: 1.401 ± 0.39
4.203SerAsn: 4.203 ± 0.798
1.186SerPro: 1.186 ± 0.406
1.617SerGln: 1.617 ± 0.478
2.371SerArg: 2.371 ± 0.437
5.066SerSer: 5.066 ± 0.943
2.695SerThr: 2.695 ± 0.733
4.096SerVal: 4.096 ± 0.835
1.186SerTrp: 1.186 ± 0.411
2.048SerTyr: 2.048 ± 0.573
0.0SerXaa: 0.0 ± 0.0
Thr
4.635ThrAla: 4.635 ± 0.618
0.216ThrCys: 0.216 ± 0.148
3.557ThrAsp: 3.557 ± 0.71
6.036ThrGlu: 6.036 ± 0.637
2.695ThrPhe: 2.695 ± 0.598
3.772ThrGly: 3.772 ± 0.522
0.108ThrHis: 0.108 ± 0.108
3.665ThrIle: 3.665 ± 0.799
5.174ThrLys: 5.174 ± 0.637
6.144ThrLeu: 6.144 ± 0.841
0.97ThrMet: 0.97 ± 0.305
4.742ThrAsn: 4.742 ± 0.723
1.832ThrPro: 1.832 ± 0.363
2.479ThrGln: 2.479 ± 0.482
2.048ThrArg: 2.048 ± 0.509
4.635ThrSer: 4.635 ± 0.785
4.203ThrThr: 4.203 ± 0.647
5.497ThrVal: 5.497 ± 1.01
1.186ThrTrp: 1.186 ± 0.394
2.479ThrTyr: 2.479 ± 0.59
0.0ThrXaa: 0.0 ± 0.0
Val
3.449ValAla: 3.449 ± 0.525
0.431ValCys: 0.431 ± 0.218
3.988ValAsp: 3.988 ± 0.632
5.174ValGlu: 5.174 ± 0.608
3.018ValPhe: 3.018 ± 0.635
3.772ValGly: 3.772 ± 0.689
0.539ValHis: 0.539 ± 0.208
4.635ValIle: 4.635 ± 0.666
5.497ValLys: 5.497 ± 0.773
2.91ValLeu: 2.91 ± 0.559
2.263ValMet: 2.263 ± 0.444
3.449ValAsn: 3.449 ± 0.66
1.293ValPro: 1.293 ± 0.373
1.509ValGln: 1.509 ± 0.325
2.91ValArg: 2.91 ± 0.623
5.066ValSer: 5.066 ± 1.299
5.174ValThr: 5.174 ± 0.862
3.449ValVal: 3.449 ± 0.784
0.431ValTrp: 0.431 ± 0.184
3.126ValTyr: 3.126 ± 0.487
0.0ValXaa: 0.0 ± 0.0
Trp
0.754TrpAla: 0.754 ± 0.243
0.216TrpCys: 0.216 ± 0.148
1.186TrpAsp: 1.186 ± 0.577
0.754TrpGlu: 0.754 ± 0.287
1.078TrpPhe: 1.078 ± 0.396
0.97TrpGly: 0.97 ± 0.296
0.108TrpHis: 0.108 ± 0.102
0.754TrpIle: 0.754 ± 0.268
0.862TrpLys: 0.862 ± 0.316
1.186TrpLeu: 1.186 ± 0.391
0.216TrpMet: 0.216 ± 0.135
1.293TrpAsn: 1.293 ± 0.435
0.108TrpPro: 0.108 ± 0.119
0.862TrpGln: 0.862 ± 0.303
0.323TrpArg: 0.323 ± 0.255
1.186TrpSer: 1.186 ± 0.331
0.647TrpThr: 0.647 ± 0.241
0.647TrpVal: 0.647 ± 0.289
0.216TrpTrp: 0.216 ± 0.153
0.862TrpTyr: 0.862 ± 0.286
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.617TyrAla: 1.617 ± 0.49
0.862TyrCys: 0.862 ± 0.384
2.156TyrAsp: 2.156 ± 0.553
3.449TyrGlu: 3.449 ± 0.693
2.587TyrPhe: 2.587 ± 0.487
2.695TyrGly: 2.695 ± 0.521
1.186TyrHis: 1.186 ± 0.308
2.802TyrIle: 2.802 ± 0.809
2.802TyrLys: 2.802 ± 0.705
3.665TyrLeu: 3.665 ± 0.81
0.754TyrMet: 0.754 ± 0.299
3.88TyrAsn: 3.88 ± 0.58
1.509TyrPro: 1.509 ± 0.423
1.186TyrGln: 1.186 ± 0.538
1.401TyrArg: 1.401 ± 0.366
2.263TyrSer: 2.263 ± 0.631
3.341TyrThr: 3.341 ± 0.794
2.263TyrVal: 2.263 ± 0.461
0.0TyrTrp: 0.0 ± 0.0
1.94TyrTyr: 1.94 ± 0.572
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (9279 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski