Chenopodium leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6KBA6|F6KBA6_9GEMI AC4 protein OS=Chenopodium leaf curl virus OX=1032478 GN=AC4 PE=4 SV=1
MM1 pKa = 7.62PRR3 pKa = 11.84NPNSFRR9 pKa = 11.84IQARR13 pKa = 11.84NIFLTYY19 pKa = 8.33PQCDD23 pKa = 3.41IPKK26 pKa = 10.52DD27 pKa = 3.61EE28 pKa = 5.52ALLLLQSLPWSVVKK42 pKa = 8.51PTYY45 pKa = 10.14IRR47 pKa = 11.84VARR50 pKa = 11.84EE51 pKa = 3.38EE52 pKa = 4.12HH53 pKa = 6.9ADD55 pKa = 3.74GFPHH59 pKa = 6.45LHH61 pKa = 6.82CLIQLSGKK69 pKa = 10.23SNIKK73 pKa = 9.85DD74 pKa = 3.18ARR76 pKa = 11.84FFDD79 pKa = 3.84LTHH82 pKa = 6.77PRR84 pKa = 11.84RR85 pKa = 11.84SAGFHH90 pKa = 6.69PNVQGAKK97 pKa = 8.99DD98 pKa = 3.52TNAVKK103 pKa = 10.63NYY105 pKa = 6.66ITKK108 pKa = 10.56EE109 pKa = 3.5GDD111 pKa = 3.42YY112 pKa = 10.99CEE114 pKa = 4.36SGQYY118 pKa = 9.59KK119 pKa = 10.31VSGGSKK125 pKa = 9.95ANKK128 pKa = 9.55DD129 pKa = 3.77DD130 pKa = 4.11VYY132 pKa = 11.28HH133 pKa = 6.33NAVNATTARR142 pKa = 11.84EE143 pKa = 3.8ALDD146 pKa = 3.87IIKK149 pKa = 10.77AGDD152 pKa = 3.64PKK154 pKa = 10.74TFIVSYY160 pKa = 10.64HH161 pKa = 4.93NVKK164 pKa = 10.92ANIEE168 pKa = 4.22RR169 pKa = 11.84LFHH172 pKa = 6.53KK173 pKa = 10.29PPEE176 pKa = 4.09RR177 pKa = 11.84WVPPFPLSSFTNVPVDD193 pKa = 3.55MQAWADD199 pKa = 3.72DD200 pKa = 3.81YY201 pKa = 11.53FGNGVAARR209 pKa = 11.84PEE211 pKa = 4.06RR212 pKa = 11.84PISIIVEE219 pKa = 4.05GDD221 pKa = 3.08SRR223 pKa = 11.84TGKK226 pKa = 8.55TMWARR231 pKa = 11.84ALGPHH236 pKa = 6.63NYY238 pKa = 10.14LSGHH242 pKa = 6.84LDD244 pKa = 3.54FNARR248 pKa = 11.84VYY250 pKa = 11.19SNEE253 pKa = 3.49VEE255 pKa = 4.32YY256 pKa = 11.37NVIDD260 pKa = 4.42DD261 pKa = 4.55VAPQYY266 pKa = 11.38LKK268 pKa = 10.74LKK270 pKa = 9.17HH271 pKa = 5.65WKK273 pKa = 9.52EE274 pKa = 3.61LLGAQKK280 pKa = 10.6DD281 pKa = 3.68WQSNCKK287 pKa = 9.73YY288 pKa = 10.21GRR290 pKa = 11.84PVQIKK295 pKa = 9.96GGIPSIVLCNPGEE308 pKa = 4.11GASYY312 pKa = 11.17KK313 pKa = 10.63EE314 pKa = 4.03FLDD317 pKa = 4.42KK318 pKa = 10.97IEE320 pKa = 4.19NTSLKK325 pKa = 10.53NWTIKK330 pKa = 10.53NAVFITLTSPLYY342 pKa = 10.13QDD344 pKa = 3.9SAQDD348 pKa = 3.55SQEE351 pKa = 4.22EE352 pKa = 4.42GDD354 pKa = 5.48QEE356 pKa = 4.35AQDD359 pKa = 3.55

Molecular weight: 40.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6KBA3|F6KBA3_9GEMI Replication enhancer OS=Chenopodium leaf curl virus OX=1032478 GN=REn/AC3 PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.96RR4 pKa = 11.84EE5 pKa = 4.28APWRR9 pKa = 11.84LLAGTSKK16 pKa = 10.58VSRR19 pKa = 11.84NAKK22 pKa = 9.07YY23 pKa = 8.84STRR26 pKa = 11.84LGSGPTFNKK35 pKa = 8.17ATEE38 pKa = 4.17WVNRR42 pKa = 11.84PMYY45 pKa = 9.79RR46 pKa = 11.84KK47 pKa = 8.94PRR49 pKa = 11.84IYY51 pKa = 10.95RR52 pKa = 11.84MIRR55 pKa = 11.84TPDD58 pKa = 3.22VPRR61 pKa = 11.84GCEE64 pKa = 4.34GPCKK68 pKa = 10.21VQSFEE73 pKa = 3.83QRR75 pKa = 11.84HH76 pKa = 5.51DD77 pKa = 3.54VSHH80 pKa = 6.24TGKK83 pKa = 10.47VICISDD89 pKa = 3.51VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.16ILGKK114 pKa = 9.37IWMDD118 pKa = 3.39EE119 pKa = 4.0NIKK122 pKa = 10.61LKK124 pKa = 10.73NHH126 pKa = 5.95TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.7RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMFDD155 pKa = 3.99NEE157 pKa = 4.11PSTATVKK164 pKa = 10.81NDD166 pKa = 2.84LRR168 pKa = 11.84DD169 pKa = 3.6RR170 pKa = 11.84YY171 pKa = 10.16QVMHH175 pKa = 6.95RR176 pKa = 11.84FYY178 pKa = 11.43GKK180 pKa = 8.6VTGGQYY186 pKa = 11.07ASNEE190 pKa = 3.69QAIVKK195 pKa = 9.05RR196 pKa = 11.84FWKK199 pKa = 10.22VNNHH203 pKa = 4.48VVYY206 pKa = 10.67NHH208 pKa = 5.8QEE210 pKa = 3.47AGKK213 pKa = 10.41YY214 pKa = 8.39EE215 pKa = 4.09NHH217 pKa = 6.52TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.86ATLKK240 pKa = 9.47IRR242 pKa = 11.84IYY244 pKa = 10.6FYY246 pKa = 11.26DD247 pKa = 4.02SIMNN251 pKa = 4.24

Molecular weight: 29.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1139

121

359

189.8

21.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.092 ± 1.056

1.844 ± 0.336

4.917 ± 0.783

3.775 ± 0.676

4.478 ± 0.488

6.234 ± 0.878

3.424 ± 0.552

5.795 ± 0.358

5.18 ± 1.12

7.902 ± 1.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.555

5.619 ± 0.782

5.97 ± 0.77

3.775 ± 0.432

7.199 ± 0.899

7.989 ± 1.237

6.497 ± 1.194

6.497 ± 0.596

1.668 ± 0.216

3.863 ± 0.643
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski