Mycobacterium phage Misha28

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1N2Z0|A0A2P1N2Z0_9CAUD Uncharacterized protein OS=Mycobacterium phage Misha28 OX=2126810 GN=79 PE=4 SV=1
MM1 pKa = 7.24TALNYY6 pKa = 10.86DD7 pKa = 3.81EE8 pKa = 5.43TPCALCDD15 pKa = 4.32CYY17 pKa = 11.21GLQHH21 pKa = 7.56DD22 pKa = 4.88DD23 pKa = 4.18PEE25 pKa = 6.27HH26 pKa = 6.52GGPCRR31 pKa = 11.84ALDD34 pKa = 3.85GHH36 pKa = 6.57GLPCGCPGFEE46 pKa = 4.15EE47 pKa = 5.43PEE49 pKa = 4.41DD50 pKa = 3.77EE51 pKa = 4.43TT52 pKa = 5.06

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1N2V6|A0A2P1N2V6_9CAUD Uncharacterized protein OS=Mycobacterium phage Misha28 OX=2126810 GN=24 PE=4 SV=1
MM1 pKa = 7.4LTRR4 pKa = 11.84SFWIDD9 pKa = 2.95AVEE12 pKa = 3.85RR13 pKa = 11.84AARR16 pKa = 11.84TFAQTAIAALGAGAVDD32 pKa = 4.35LLKK35 pKa = 10.09TDD37 pKa = 3.38WVSVLSVSGGAAVVSLLMSIAAEE60 pKa = 3.82RR61 pKa = 11.84RR62 pKa = 11.84GNPGTASATRR72 pKa = 11.84AVTAAA77 pKa = 3.71

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

18374

23

1176

197.6

21.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.239 ± 0.472

1.11 ± 0.168

6.803 ± 0.283

5.638 ± 0.247

2.89 ± 0.174

9.143 ± 0.615

2.27 ± 0.203

4.316 ± 0.205

3.162 ± 0.194

7.173 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.063 ± 0.112

3.129 ± 0.204

5.894 ± 0.177

3.646 ± 0.214

6.988 ± 0.461

5.9 ± 0.318

6.498 ± 0.222

7.336 ± 0.271

2.177 ± 0.136

2.623 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski