Methanobrevibacter woesei

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobrevibacter

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1581 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U1S9R8|A0A2U1S9R8_9EURY Uncharacterized protein OS=Methanobrevibacter woesei OX=190976 GN=MBBWO_02570 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84KK3 pKa = 9.41AVIGIIVVVILIAGGVGLSANSINNGNNNSTVITYY38 pKa = 11.0GEE40 pKa = 4.05TTFANDD46 pKa = 3.17QYY48 pKa = 11.64KK49 pKa = 10.73SLVDD53 pKa = 3.61SYY55 pKa = 11.83FMNNNYY61 pKa = 10.26GNLEE65 pKa = 3.98SMNYY69 pKa = 10.1KK70 pKa = 10.2IITADD75 pKa = 3.3EE76 pKa = 4.29VNAISQGISQEE87 pKa = 4.56TYY89 pKa = 9.91SSGQIFSSSLVDD101 pKa = 4.18LGSSEE106 pKa = 4.76DD107 pKa = 3.57LSIDD111 pKa = 3.32VDD113 pKa = 3.62HH114 pKa = 7.27SKK116 pKa = 8.56ITLVTDD122 pKa = 3.9DD123 pKa = 4.36MYY125 pKa = 11.69KK126 pKa = 10.34SALEE130 pKa = 3.97STGITKK136 pKa = 9.98GHH138 pKa = 6.51VIVTSPVSATGEE150 pKa = 4.08SALAGIMSAYY160 pKa = 9.85EE161 pKa = 3.77YY162 pKa = 9.68ATNTEE167 pKa = 3.69IPEE170 pKa = 4.27EE171 pKa = 4.1VKK173 pKa = 10.51EE174 pKa = 4.02AANEE178 pKa = 4.15EE179 pKa = 4.7IYY181 pKa = 8.06TTSEE185 pKa = 3.46IVNNSNVSADD195 pKa = 3.83DD196 pKa = 3.55LTDD199 pKa = 3.33VVDD202 pKa = 4.03EE203 pKa = 4.41VKK205 pKa = 11.09EE206 pKa = 3.9EE207 pKa = 4.13VEE209 pKa = 3.97AQNITNHH216 pKa = 4.74EE217 pKa = 4.39TIVNIINNVTVNYY230 pKa = 10.37NIVLSPEE237 pKa = 4.59DD238 pKa = 3.64IEE240 pKa = 5.03NLAEE244 pKa = 5.15SIRR247 pKa = 11.84QTQLVQDD254 pKa = 3.8QASDD258 pKa = 3.7YY259 pKa = 10.57QSQINSFIQNNTGGFSLEE277 pKa = 4.31NIFNFF282 pKa = 4.37

Molecular weight: 30.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U1S6E9|A0A2U1S6E9_9EURY Copper-exporting P-type ATPase A OS=Methanobrevibacter woesei OX=190976 GN=copA_1 PE=3 SV=1
MM1 pKa = 7.51SRR3 pKa = 11.84NKK5 pKa = 9.92PLAKK9 pKa = 9.48KK10 pKa = 10.5LRR12 pKa = 11.84MAKK15 pKa = 10.13ANKK18 pKa = 7.84QNRR21 pKa = 11.84RR22 pKa = 11.84IPIWAYY28 pKa = 10.35AKK30 pKa = 9.41TNRR33 pKa = 11.84KK34 pKa = 8.76LRR36 pKa = 11.84YY37 pKa = 8.95RR38 pKa = 11.84PKK40 pKa = 8.62PRR42 pKa = 11.84HH43 pKa = 4.36WRR45 pKa = 11.84RR46 pKa = 11.84NSLKK50 pKa = 10.73LL51 pKa = 3.43

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1581

0

1581

459352

29

2108

290.5

32.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.005 ± 0.072

1.294 ± 0.033

6.582 ± 0.05

7.98 ± 0.072

4.321 ± 0.055

6.417 ± 0.07

1.603 ± 0.025

9.529 ± 0.068

7.985 ± 0.074

8.846 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.03

6.019 ± 0.083

3.374 ± 0.035

2.175 ± 0.026

3.239 ± 0.044

6.32 ± 0.052

4.868 ± 0.044

6.642 ± 0.063

0.629 ± 0.016

3.791 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski