Corynebacterium heidelbergense
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1960 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A364V7Z4|A0A364V7Z4_9CORY DUF2993 domain-containing protein OS=Corynebacterium heidelbergense OX=2055947 GN=DLJ54_01785 PE=4 SV=1
MM1 pKa = 7.57 LNGPTTFDD9 pKa = 3.24 VDD11 pKa = 3.73 GMSCEE16 pKa = 3.98 HH17 pKa = 7.0 CVRR20 pKa = 11.84 AVTNAVSAIPGASDD34 pKa = 3.14 VRR36 pKa = 11.84 VDD38 pKa = 4.05 LGSGTVAFVAADD50 pKa = 3.65 VPEE53 pKa = 4.32 VAVVDD58 pKa = 5.22 AIDD61 pKa = 3.99 DD62 pKa = 3.92 AGYY65 pKa = 10.58 DD66 pKa = 3.69 ATLHH70 pKa = 5.98 HH71 pKa = 7.05 APP73 pKa = 4.36
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.948
IPC_protein 3.884
Toseland 3.668
ProMoST 4.101
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.719
Grimsley 3.592
Solomon 3.884
Lehninger 3.846
Nozaki 4.05
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.91
Sillero 4.024
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A364V8G2|A0A364V8G2_9CORY Fumarate reductase/succinate dehydrogenase flavoprotein subunit OS=Corynebacterium heidelbergense OX=2055947 GN=DLJ54_01085 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AVVSARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.2 GRR42 pKa = 11.84 KK43 pKa = 6.84 TLTAA47 pKa = 4.25
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.735
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.457
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.26
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1960
0
1960
659679
30
2172
336.6
36.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.171 ± 0.07
0.769 ± 0.015
5.741 ± 0.049
6.033 ± 0.051
3.055 ± 0.032
9.038 ± 0.053
2.274 ± 0.026
4.389 ± 0.043
2.847 ± 0.043
9.308 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.139 ± 0.024
2.629 ± 0.034
5.616 ± 0.045
3.642 ± 0.034
6.978 ± 0.056
5.78 ± 0.039
5.852 ± 0.035
8.22 ± 0.046
1.395 ± 0.024
2.123 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here