Streptococcus satellite phage Javan518

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZQD9|A0A4D5ZQD9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan518 OX=2558728 GN=JavanS518_0013 PE=4 SV=1
MM1 pKa = 7.72AKK3 pKa = 10.2NSPQDD8 pKa = 3.86LINRR12 pKa = 11.84EE13 pKa = 3.91IFSTNLNMLMAKK25 pKa = 10.2KK26 pKa = 10.1NIKK29 pKa = 10.21QIDD32 pKa = 3.46IHH34 pKa = 6.66NKK36 pKa = 9.74LGIPKK41 pKa = 8.5STITGYY47 pKa = 10.91VKK49 pKa = 10.57GRR51 pKa = 11.84SLPTAGNVQKK61 pKa = 10.8LADD64 pKa = 3.93FFGVLKK70 pKa = 10.84SDD72 pKa = 3.19IDD74 pKa = 3.86PRR76 pKa = 11.84FDD78 pKa = 4.03SNNIEE83 pKa = 4.29TNSNIIPSTLQKK95 pKa = 9.47VTSTLSQLEE104 pKa = 4.51HH105 pKa = 6.54KK106 pKa = 10.36RR107 pKa = 11.84QLNVLDD113 pKa = 3.87YY114 pKa = 11.66AEE116 pKa = 4.47TQLEE120 pKa = 4.14QQNTVEE126 pKa = 4.75EE127 pKa = 4.39PQATYY132 pKa = 8.87YY133 pKa = 9.48TYY135 pKa = 11.16NYY137 pKa = 9.02YY138 pKa = 11.1DD139 pKa = 3.61HH140 pKa = 7.34AASAGTGQYY149 pKa = 11.03LNDD152 pKa = 3.69VQVEE156 pKa = 4.58TIEE159 pKa = 5.25LPVDD163 pKa = 3.45YY164 pKa = 10.6DD165 pKa = 3.94ADD167 pKa = 3.98FVIPVYY173 pKa = 10.34GDD175 pKa = 3.3SMEE178 pKa = 4.56PEE180 pKa = 4.48YY181 pKa = 11.08HH182 pKa = 6.24SGDD185 pKa = 3.53YY186 pKa = 11.17VFIKK190 pKa = 10.62LSINLSDD197 pKa = 3.52GDD199 pKa = 3.54IGVFEE204 pKa = 4.94YY205 pKa = 11.34YY206 pKa = 10.64GDD208 pKa = 4.18AYY210 pKa = 9.96IKK212 pKa = 10.44QLVINDD218 pKa = 3.53SGAFLHH224 pKa = 6.49SLNDD228 pKa = 3.42KK229 pKa = 10.89YY230 pKa = 11.69DD231 pKa = 5.24DD232 pKa = 3.68ILIDD236 pKa = 3.7RR237 pKa = 11.84DD238 pKa = 3.23SDD240 pKa = 3.49FRR242 pKa = 11.84IIGEE246 pKa = 4.32VIGSFTSKK254 pKa = 10.46SS255 pKa = 3.33

Molecular weight: 28.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZUR7|A0A4D5ZUR7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan518 OX=2558728 GN=JavanS518_0012 PE=4 SV=1
MM1 pKa = 7.57SGGGAYY7 pKa = 9.43VCKK10 pKa = 10.2SQKK13 pKa = 9.01PKK15 pKa = 9.91EE16 pKa = 4.06RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84QGLSVYY25 pKa = 7.65EE26 pKa = 4.27TLTLMIAFGTLIVAIMNNKK45 pKa = 8.89NKK47 pKa = 10.56

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

3520

40

464

140.8

16.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.625 ± 0.358

0.909 ± 0.239

5.653 ± 0.512

7.244 ± 0.575

3.722 ± 0.212

5.17 ± 0.364

1.96 ± 0.273

6.619 ± 0.375

8.807 ± 0.503

9.801 ± 0.62

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.102 ± 0.22

5.54 ± 0.397

3.494 ± 0.458

4.517 ± 0.247

5.568 ± 0.424

5.455 ± 0.342

6.392 ± 0.324

5.17 ± 0.341

0.966 ± 0.182

5.284 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski