Amino acid dipepetide frequency for Methylobacterium sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.425AlaAla: 20.425 ± 0.174	1.251AlaCys: 1.251 ± 0.034	7.379AlaAsp: 7.379 ± 0.072	8.366AlaGlu: 8.366 ± 0.089	4.731AlaPhe: 4.731 ± 0.064	12.683AlaGly: 12.683 ± 0.114	2.482AlaHis: 2.482 ± 0.045	6.21AlaIle: 6.21 ± 0.067	3.715AlaLys: 3.715 ± 0.059	15.146AlaLeu: 15.146 ± 0.125	3.59AlaMet: 3.59 ± 0.052	2.536AlaAsn: 2.536 ± 0.048	6.82AlaPro: 6.82 ± 0.077	4.265AlaGln: 4.265 ± 0.063	10.718AlaArg: 10.718 ± 0.095	6.35AlaSer: 6.35 ± 0.082	6.898AlaThr: 6.898 ± 0.082	10.216AlaVal: 10.216 ± 0.1	1.808AlaTrp: 1.808 ± 0.04	2.99AlaTyr: 2.99 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	1.033CysAla: 1.033 ± 0.028	0.087CysCys: 0.087 ± 0.007	0.531CysAsp: 0.531 ± 0.02	0.404CysGlu: 0.404 ± 0.016	0.265CysPhe: 0.265 ± 0.015	0.96CysGly: 0.96 ± 0.027	0.215CysHis: 0.215 ± 0.015	0.346CysIle: 0.346 ± 0.015	0.139CysLys: 0.139 ± 0.01	0.929CysLeu: 0.929 ± 0.027	0.157CysMet: 0.157 ± 0.01	0.183CysAsn: 0.183 ± 0.012	0.48CysPro: 0.48 ± 0.021	0.189CysGln: 0.189 ± 0.011	0.667CysArg: 0.667 ± 0.025	0.382CysSer: 0.382 ± 0.016	0.501CysThr: 0.501 ± 0.02	0.583CysVal: 0.583 ± 0.021	0.117CysTrp: 0.117 ± 0.009	0.163CysTyr: 0.163 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.886AspAla: 7.886 ± 0.084	0.41AspCys: 0.41 ± 0.019	2.937AspAsp: 2.937 ± 0.055	2.994AspGlu: 2.994 ± 0.049	1.889AspPhe: 1.889 ± 0.04	5.696AspGly: 5.696 ± 0.063	1.249AspHis: 1.249 ± 0.031	2.419AspIle: 2.419 ± 0.049	1.328AspLys: 1.328 ± 0.036	6.491AspLeu: 6.491 ± 0.074	1.108AspMet: 1.108 ± 0.026	0.972AspAsn: 0.972 ± 0.028	4.102AspPro: 4.102 ± 0.054	1.429AspGln: 1.429 ± 0.033	4.799AspArg: 4.799 ± 0.063	1.93AspSer: 1.93 ± 0.041	2.619AspThr: 2.619 ± 0.043	4.134AspVal: 4.134 ± 0.058	0.9AspTrp: 0.9 ± 0.029	1.31AspTyr: 1.31 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	8.898GluAla: 8.898 ± 0.111	0.3GluCys: 0.3 ± 0.014	2.481GluAsp: 2.481 ± 0.046	2.446GluGlu: 2.446 ± 0.052	1.314GluPhe: 1.314 ± 0.028	4.468GluGly: 4.468 ± 0.069	1.128GluHis: 1.128 ± 0.03	2.968GluIle: 2.968 ± 0.052	1.54GluLys: 1.54 ± 0.039	3.968GluLeu: 3.968 ± 0.059	1.34GluMet: 1.34 ± 0.03	1.128GluAsn: 1.128 ± 0.029	3.001GluPro: 3.001 ± 0.051	1.579GluGln: 1.579 ± 0.032	5.149GluArg: 5.149 ± 0.078	2.202GluSer: 2.202 ± 0.04	3.535GluThr: 3.535 ± 0.055	3.718GluVal: 3.718 ± 0.058	0.559GluTrp: 0.559 ± 0.022	0.819GluTyr: 0.819 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.583PheAla: 4.583 ± 0.061	0.375PheCys: 0.375 ± 0.016	2.39PheAsp: 2.39 ± 0.044	1.891PheGlu: 1.891 ± 0.039	1.21PhePhe: 1.21 ± 0.036	3.697PheGly: 3.697 ± 0.055	0.701PheHis: 0.701 ± 0.025	1.182PheIle: 1.182 ± 0.03	0.85PheLys: 0.85 ± 0.021	3.283PheLeu: 3.283 ± 0.056	0.633PheMet: 0.633 ± 0.025	0.822PheAsn: 0.822 ± 0.031	1.525PhePro: 1.525 ± 0.038	0.857PheGln: 0.857 ± 0.026	2.273PheArg: 2.273 ± 0.044	1.956PheSer: 1.956 ± 0.036	1.991PheThr: 1.991 ± 0.043	2.788PheVal: 2.788 ± 0.049	0.498PheTrp: 0.498 ± 0.019	0.881PheTyr: 0.881 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	10.968GlyAla: 10.968 ± 0.088	0.862GlyCys: 0.862 ± 0.026	4.562GlyAsp: 4.562 ± 0.067	4.527GlyGlu: 4.527 ± 0.056	3.808GlyPhe: 3.808 ± 0.055	7.802GlyGly: 7.802 ± 0.105	2.049GlyHis: 2.049 ± 0.043	4.542GlyIle: 4.542 ± 0.062	2.597GlyLys: 2.597 ± 0.047	10.679GlyLeu: 10.679 ± 0.103	2.198GlyMet: 2.198 ± 0.041	1.882GlyAsn: 1.882 ± 0.046	4.455GlyPro: 4.455 ± 0.06	2.94GlyGln: 2.94 ± 0.046	7.661GlyArg: 7.661 ± 0.093	4.601GlySer: 4.601 ± 0.06	5.533GlyThr: 5.533 ± 0.07	6.49GlyVal: 6.49 ± 0.07	1.359GlyTrp: 1.359 ± 0.031	2.424GlyTyr: 2.424 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.732HisAla: 2.732 ± 0.05	0.216HisCys: 0.216 ± 0.012	1.292HisAsp: 1.292 ± 0.033	0.911HisGlu: 0.911 ± 0.029	0.705HisPhe: 0.705 ± 0.021	2.15HisGly: 2.15 ± 0.043	0.618HisHis: 0.618 ± 0.028	0.688HisIle: 0.688 ± 0.023	0.376HisLys: 0.376 ± 0.017	2.103HisLeu: 2.103 ± 0.046	0.406HisMet: 0.406 ± 0.019	0.394HisAsn: 0.394 ± 0.017	1.493HisPro: 1.493 ± 0.031	0.517HisGln: 0.517 ± 0.019	1.636HisArg: 1.636 ± 0.033	0.727HisSer: 0.727 ± 0.022	0.868HisThr: 0.868 ± 0.026	1.587HisVal: 1.587 ± 0.031	0.322HisTrp: 0.322 ± 0.015	0.52HisTyr: 0.52 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.842IleAla: 6.842 ± 0.084	0.438IleCys: 0.438 ± 0.022	3.111IleAsp: 3.111 ± 0.05	3.04IleGlu: 3.04 ± 0.051	1.235IlePhe: 1.235 ± 0.036	4.658IleGly: 4.658 ± 0.059	0.854IleHis: 0.854 ± 0.024	1.548IleIle: 1.548 ± 0.036	1.077IleLys: 1.077 ± 0.03	4.433IleLeu: 4.433 ± 0.07	0.748IleMet: 0.748 ± 0.025	1.045IleAsn: 1.045 ± 0.03	2.224IlePro: 2.224 ± 0.046	1.121IleGln: 1.121 ± 0.029	3.229IleArg: 3.229 ± 0.051	1.947IleSer: 1.947 ± 0.043	2.488IleThr: 2.488 ± 0.041	3.938IleVal: 3.938 ± 0.049	0.495IleTrp: 0.495 ± 0.021	0.915IleTyr: 0.915 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	3.897LysAla: 3.897 ± 0.065	0.106LysCys: 0.106 ± 0.009	1.456LysAsp: 1.456 ± 0.036	1.091LysGlu: 1.091 ± 0.034	0.681LysPhe: 0.681 ± 0.024	2.428LysGly: 2.428 ± 0.048	0.435LysHis: 0.435 ± 0.018	1.225LysIle: 1.225 ± 0.03	0.835LysLys: 0.835 ± 0.032	2.506LysLeu: 2.506 ± 0.044	0.529LysMet: 0.529 ± 0.022	0.642LysAsn: 0.642 ± 0.025	1.927LysPro: 1.927 ± 0.04	0.731LysGln: 0.731 ± 0.024	1.99LysArg: 1.99 ± 0.039	1.344LysSer: 1.344 ± 0.031	1.542LysThr: 1.542 ± 0.033	2.116LysVal: 2.116 ± 0.047	0.255LysTrp: 0.255 ± 0.014	0.495LysTyr: 0.495 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	15.206LeuAla: 15.206 ± 0.131	0.978LeuCys: 0.978 ± 0.029	6.41LeuAsp: 6.41 ± 0.074	4.643LeuGlu: 4.643 ± 0.065	3.502LeuPhe: 3.502 ± 0.064	9.185LeuGly: 9.185 ± 0.102	2.013LeuHis: 2.013 ± 0.039	4.517LeuIle: 4.517 ± 0.062	2.992LeuLys: 2.992 ± 0.047	9.044LeuLeu: 9.044 ± 0.114	2.232LeuMet: 2.232 ± 0.044	2.231LeuAsn: 2.231 ± 0.043	5.751LeuPro: 5.751 ± 0.068	2.557LeuGln: 2.557 ± 0.046	7.595LeuArg: 7.595 ± 0.081	5.826LeuSer: 5.826 ± 0.07	5.716LeuThr: 5.716 ± 0.07	8.411LeuVal: 8.411 ± 0.084	1.18LeuTrp: 1.18 ± 0.029	2.234LeuTyr: 2.234 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.934MetAla: 2.934 ± 0.043	0.145MetCys: 0.145 ± 0.011	0.992MetAsp: 0.992 ± 0.029	0.968MetGlu: 0.968 ± 0.03	0.578MetPhe: 0.578 ± 0.021	1.731MetGly: 1.731 ± 0.041	0.432MetHis: 0.432 ± 0.018	1.147MetIle: 1.147 ± 0.031	0.749MetLys: 0.749 ± 0.022	2.309MetLeu: 2.309 ± 0.047	0.627MetMet: 0.627 ± 0.024	0.624MetAsn: 0.624 ± 0.021	1.564MetPro: 1.564 ± 0.034	0.725MetGln: 0.725 ± 0.025	2.03MetArg: 2.03 ± 0.041	1.396MetSer: 1.396 ± 0.033	1.706MetThr: 1.706 ± 0.036	1.581MetVal: 1.581 ± 0.035	0.187MetTrp: 0.187 ± 0.012	0.284MetTyr: 0.284 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.926AsnAla: 2.926 ± 0.04	0.214AsnCys: 0.214 ± 0.013	1.106AsnAsp: 1.106 ± 0.029	0.893AsnGlu: 0.893 ± 0.027	0.684AsnPhe: 0.684 ± 0.025	2.131AsnGly: 2.131 ± 0.043	0.44AsnHis: 0.44 ± 0.017	0.928AsnIle: 0.928 ± 0.027	0.529AsnLys: 0.529 ± 0.02	2.256AsnLeu: 2.256 ± 0.045	0.505AsnMet: 0.505 ± 0.02	0.585AsnAsn: 0.585 ± 0.021	1.696AsnPro: 1.696 ± 0.037	0.597AsnGln: 0.597 ± 0.022	1.627AsnArg: 1.627 ± 0.029	0.85AsnSer: 0.85 ± 0.027	1.182AsnThr: 1.182 ± 0.033	1.67AsnVal: 1.67 ± 0.037	0.346AsnTrp: 0.346 ± 0.016	0.532AsnTyr: 0.532 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.68ProAla: 7.68 ± 0.093	0.401ProCys: 0.401 ± 0.018	4.146ProAsp: 4.146 ± 0.061	4.02ProGlu: 4.02 ± 0.053	2.034ProPhe: 2.034 ± 0.035	5.534ProGly: 5.534 ± 0.067	1.105ProHis: 1.105 ± 0.029	2.287ProIle: 2.287 ± 0.043	1.573ProLys: 1.573 ± 0.035	4.828ProLeu: 4.828 ± 0.052	1.255ProMet: 1.255 ± 0.028	1.353ProAsn: 1.353 ± 0.029	2.997ProPro: 2.997 ± 0.054	1.485ProGln: 1.485 ± 0.038	3.593ProArg: 3.593 ± 0.055	2.802ProSer: 2.802 ± 0.047	2.699ProThr: 2.699 ± 0.046	4.816ProVal: 4.816 ± 0.072	0.767ProTrp: 0.767 ± 0.023	1.257ProTyr: 1.257 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.526GlnAla: 4.526 ± 0.06	0.183GlnCys: 0.183 ± 0.012	1.474GlnAsp: 1.474 ± 0.031	1.215GlnGlu: 1.215 ± 0.029	0.867GlnPhe: 0.867 ± 0.025	2.623GlnGly: 2.623 ± 0.047	0.536GlnHis: 0.536 ± 0.023	1.478GlnIle: 1.478 ± 0.04	0.742GlnLys: 0.742 ± 0.023	2.272GlnLeu: 2.272 ± 0.042	0.652GlnMet: 0.652 ± 0.024	0.658GlnAsn: 0.658 ± 0.023	1.603GlnPro: 1.603 ± 0.037	0.835GlnGln: 0.835 ± 0.032	2.171GlnArg: 2.171 ± 0.042	1.446GlnSer: 1.446 ± 0.029	1.622GlnThr: 1.622 ± 0.037	2.374GlnVal: 2.374 ± 0.045	0.306GlnTrp: 0.306 ± 0.016	0.555GlnTyr: 0.555 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	9.849ArgAla: 9.849 ± 0.102	0.498ArgCys: 0.498 ± 0.02	4.62ArgAsp: 4.62 ± 0.059	4.066ArgGlu: 4.066 ± 0.061	3.075ArgPhe: 3.075 ± 0.05	5.655ArgGly: 5.655 ± 0.069	1.794ArgHis: 1.794 ± 0.037	4.241ArgIle: 4.241 ± 0.054	1.84ArgLys: 1.84 ± 0.039	8.677ArgLeu: 8.677 ± 0.094	1.891ArgMet: 1.891 ± 0.036	1.683ArgAsn: 1.683 ± 0.038	4.482ArgPro: 4.482 ± 0.063	2.251ArgGln: 2.251 ± 0.042	6.885ArgArg: 6.885 ± 0.086	3.799ArgSer: 3.799 ± 0.055	4.28ArgThr: 4.28 ± 0.058	5.702ArgVal: 5.702 ± 0.067	1.004ArgTrp: 1.004 ± 0.028	1.759ArgTyr: 1.759 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.194SerAla: 6.194 ± 0.073	0.373SerCys: 0.373 ± 0.02	2.64SerAsp: 2.64 ± 0.044	2.466SerGlu: 2.466 ± 0.049	1.903SerPhe: 1.903 ± 0.041	5.365SerGly: 5.365 ± 0.06	0.91SerHis: 0.91 ± 0.026	2.078SerIle: 2.078 ± 0.046	1.195SerLys: 1.195 ± 0.032	5.101SerLeu: 5.101 ± 0.059	1.07SerMet: 1.07 ± 0.031	1.021SerAsn: 1.021 ± 0.033	2.878SerPro: 2.878 ± 0.049	1.301SerGln: 1.301 ± 0.032	3.431SerArg: 3.431 ± 0.049	2.269SerSer: 2.269 ± 0.054	2.597SerThr: 2.597 ± 0.039	3.593SerVal: 3.593 ± 0.047	0.677SerTrp: 0.677 ± 0.022	1.195SerTyr: 1.195 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	6.887ThrAla: 6.887 ± 0.092	0.464ThrCys: 0.464 ± 0.02	2.873ThrAsp: 2.873 ± 0.042	2.519ThrGlu: 2.519 ± 0.041	1.969ThrPhe: 1.969 ± 0.037	5.663ThrGly: 5.663 ± 0.066	1.043ThrHis: 1.043 ± 0.033	2.699ThrIle: 2.699 ± 0.044	1.362ThrLys: 1.362 ± 0.038	6.404ThrLeu: 6.404 ± 0.074	1.209ThrMet: 1.209 ± 0.031	1.2ThrAsn: 1.2 ± 0.033	3.395ThrPro: 3.395 ± 0.06	1.473ThrGln: 1.473 ± 0.036	3.743ThrArg: 3.743 ± 0.056	2.498ThrSer: 2.498 ± 0.042	2.876ThrThr: 2.876 ± 0.049	4.956ThrVal: 4.956 ± 0.072	0.749ThrTrp: 0.749 ± 0.025	1.36ThrTyr: 1.36 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.765ValAla: 10.765 ± 0.099	0.669ValCys: 0.669 ± 0.022	4.063ValAsp: 4.063 ± 0.057	4.451ValGlu: 4.451 ± 0.064	2.655ValPhe: 2.655 ± 0.046	6.266ValGly: 6.266 ± 0.08	1.511ValHis: 1.511 ± 0.033	3.563ValIle: 3.563 ± 0.049	1.923ValLys: 1.923 ± 0.036	7.982ValLeu: 7.982 ± 0.075	1.84ValMet: 1.84 ± 0.035	1.812ValAsn: 1.812 ± 0.038	4.449ValPro: 4.449 ± 0.055	2.229ValGln: 2.229 ± 0.048	5.72ValArg: 5.72 ± 0.074	4.124ValSer: 4.124 ± 0.053	4.718ValThr: 4.718 ± 0.067	6.657ValVal: 6.657 ± 0.077	0.871ValTrp: 0.871 ± 0.024	1.486ValTyr: 1.486 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.351TrpAla: 1.351 ± 0.032	0.138TrpCys: 0.138 ± 0.01	0.683TrpAsp: 0.683 ± 0.024	0.494TrpGlu: 0.494 ± 0.017	0.511TrpPhe: 0.511 ± 0.02	0.896TrpGly: 0.896 ± 0.026	0.318TrpHis: 0.318 ± 0.017	0.606TrpIle: 0.606 ± 0.02	0.343TrpLys: 0.343 ± 0.018	1.5TrpLeu: 1.5 ± 0.038	0.336TrpMet: 0.336 ± 0.018	0.388TrpAsn: 0.388 ± 0.016	0.705TrpPro: 0.705 ± 0.024	0.426TrpGln: 0.426 ± 0.016	1.267TrpArg: 1.267 ± 0.035	0.842TrpSer: 0.842 ± 0.028	0.849TrpThr: 0.849 ± 0.027	0.767TrpVal: 0.767 ± 0.025	0.24TrpTrp: 0.24 ± 0.013	0.287TrpTyr: 0.287 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.997TyrAla: 2.997 ± 0.052	0.224TyrCys: 0.224 ± 0.013	1.456TyrAsp: 1.456 ± 0.029	1.135TyrGlu: 1.135 ± 0.032	0.77TyrPhe: 0.77 ± 0.025	2.351TyrGly: 2.351 ± 0.044	0.429TyrHis: 0.429 ± 0.019	0.715TyrIle: 0.715 ± 0.023	0.524TyrLys: 0.524 ± 0.022	2.313TyrLeu: 2.313 ± 0.04	0.382TyrMet: 0.382 ± 0.015	0.539TyrAsn: 0.539 ± 0.021	1.085TyrPro: 1.085 ± 0.031	0.628TyrGln: 0.628 ± 0.023	1.866TyrArg: 1.866 ± 0.042	0.966TyrSer: 0.966 ± 0.028	1.122TyrThr: 1.122 ± 0.03	1.658TyrVal: 1.658 ± 0.035	0.307TyrTrp: 0.307 ± 0.015	0.528TyrTyr: 0.528 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6219 proteins (1327713 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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