Nyssa sinensis

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; Cornales; Nyssaceae; Nyssa

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5J4ZMK4|A0A5J4ZMK4_9ASTE Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_014215 PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.17KK3 pKa = 10.4CEE5 pKa = 4.33LCNSTIARR13 pKa = 11.84MYY15 pKa = 10.13CDD17 pKa = 3.39SDD19 pKa = 3.78EE20 pKa = 6.42ASLCWDD26 pKa = 4.4CDD28 pKa = 4.26TKK30 pKa = 11.42VHH32 pKa = 5.84TANFLVAKK40 pKa = 10.23HH41 pKa = 6.1SRR43 pKa = 11.84TLLCHH48 pKa = 6.85ACQSPTPWTGSGPKK62 pKa = 9.9LGPTVSVCEE71 pKa = 3.95TCARR75 pKa = 11.84TNHH78 pKa = 6.6PKK80 pKa = 10.91NNDD83 pKa = 4.64DD84 pKa = 5.2EE85 pKa = 7.42DD86 pKa = 5.94DD87 pKa = 4.28DD88 pKa = 5.14QNSEE92 pKa = 4.16DD93 pKa = 5.42QSDD96 pKa = 3.89EE97 pKa = 5.54DD98 pKa = 5.86DD99 pKa = 5.98DD100 pKa = 6.76SDD102 pKa = 6.11DD103 pKa = 6.04DD104 pKa = 6.03GDD106 pKa = 5.75DD107 pKa = 3.88GDD109 pKa = 5.09GDD111 pKa = 4.61GDD113 pKa = 5.15GDD115 pKa = 6.07DD116 pKa = 6.23DD117 pKa = 7.09DD118 pKa = 7.78DD119 pKa = 7.56DD120 pKa = 7.2DD121 pKa = 7.32DD122 pKa = 6.31GDD124 pKa = 4.02NQVVPLSYY132 pKa = 10.85SSLPPPPLVSSSCSEE147 pKa = 3.88EE148 pKa = 4.25SSSKK152 pKa = 10.78LL153 pKa = 3.38

Molecular weight:
16.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5J5C084|A0A5J5C084_9ASTE RING-type domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_000362 PE=4 SV=1
MM1 pKa = 7.58SGFKK5 pKa = 9.79IHH7 pKa = 6.94GPLGPLPVPLVQQHH21 pKa = 6.27KK22 pKa = 10.31APSRR26 pKa = 11.84ALNWPQIRR34 pKa = 11.84AVQYY38 pKa = 9.61GSCDD42 pKa = 3.57CPASTLLFFQLTILVGRR59 pKa = 11.84DD60 pKa = 2.79SRR62 pKa = 11.84RR63 pKa = 11.84FLRR66 pKa = 11.84TARR69 pKa = 11.84RR70 pKa = 11.84SSLGCPNFKK79 pKa = 10.58ASNAAALAKK88 pKa = 10.58SVDD91 pKa = 3.26KK92 pKa = 10.81RR93 pKa = 11.84ATAFTVPLSTSWCFFRR109 pKa = 11.84NLKK112 pKa = 8.79GHH114 pKa = 6.52LMEE117 pKa = 5.53EE118 pKa = 4.25PLLNLLGEE126 pKa = 4.77GSWQVGYY133 pKa = 10.42ILPRR137 pKa = 11.84RR138 pKa = 11.84FSMLYY143 pKa = 9.75EE144 pKa = 4.1LLNGKK149 pKa = 9.57LGFNQLLSRR158 pKa = 4.53

Molecular weight:
17.65 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36118

0

36118

14223905

9

7775

393.8

43.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.661 ± 0.01

1.89 ± 0.006

5.255 ± 0.009

6.473 ± 0.015

4.191 ± 0.008

6.723 ± 0.013

2.393 ± 0.006

5.4 ± 0.01

5.913 ± 0.011

9.735 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.005

4.478 ± 0.009

4.943 ± 0.014

3.738 ± 0.008

5.292 ± 0.011

8.925 ± 0.016

4.928 ± 0.008

6.51 ± 0.007

1.33 ± 0.005

2.749 ± 0.007

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski