Nyssa sinensis
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J4ZMK4|A0A5J4ZMK4_9ASTE Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_014215 PE=3 SV=1
MM1 pKa = 7.52 KK2 pKa = 10.17 KK3 pKa = 10.4 CEE5 pKa = 4.33 LCNSTIARR13 pKa = 11.84 MYY15 pKa = 10.13 CDD17 pKa = 3.39 SDD19 pKa = 3.78 EE20 pKa = 6.42 ASLCWDD26 pKa = 4.4 CDD28 pKa = 4.26 TKK30 pKa = 11.42 VHH32 pKa = 5.84 TANFLVAKK40 pKa = 10.23 HH41 pKa = 6.1 SRR43 pKa = 11.84 TLLCHH48 pKa = 6.85 ACQSPTPWTGSGPKK62 pKa = 9.9 LGPTVSVCEE71 pKa = 3.95 TCARR75 pKa = 11.84 TNHH78 pKa = 6.6 PKK80 pKa = 10.91 NNDD83 pKa = 4.64 DD84 pKa = 5.2 EE85 pKa = 7.42 DD86 pKa = 5.94 DD87 pKa = 4.28 DD88 pKa = 5.14 QNSEE92 pKa = 4.16 DD93 pKa = 5.42 QSDD96 pKa = 3.89 EE97 pKa = 5.54 DD98 pKa = 5.86 DD99 pKa = 5.98 DD100 pKa = 6.76 SDD102 pKa = 6.11 DD103 pKa = 6.04 DD104 pKa = 6.03 GDD106 pKa = 5.75 DD107 pKa = 3.88 GDD109 pKa = 5.09 GDD111 pKa = 4.61 GDD113 pKa = 5.15 GDD115 pKa = 6.07 DD116 pKa = 6.23 DD117 pKa = 7.09 DD118 pKa = 7.78 DD119 pKa = 7.56 DD120 pKa = 7.2 DD121 pKa = 7.32 DD122 pKa = 6.31 GDD124 pKa = 4.02 NQVVPLSYY132 pKa = 10.85 SSLPPPPLVSSSCSEE147 pKa = 3.88 EE148 pKa = 4.25 SSSKK152 pKa = 10.78 LL153 pKa = 3.38
Molecular weight: 16.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.674
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.478
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.376
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.151
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.846
Patrickios 1.163
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A5J5C084|A0A5J5C084_9ASTE RING-type domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_000362 PE=4 SV=1
MM1 pKa = 7.58 SGFKK5 pKa = 9.79 IHH7 pKa = 6.94 GPLGPLPVPLVQQHH21 pKa = 6.27 KK22 pKa = 10.31 APSRR26 pKa = 11.84 ALNWPQIRR34 pKa = 11.84 AVQYY38 pKa = 9.61 GSCDD42 pKa = 3.57 CPASTLLFFQLTILVGRR59 pKa = 11.84 DD60 pKa = 2.79 SRR62 pKa = 11.84 RR63 pKa = 11.84 FLRR66 pKa = 11.84 TARR69 pKa = 11.84 RR70 pKa = 11.84 SSLGCPNFKK79 pKa = 10.58 ASNAAALAKK88 pKa = 10.58 SVDD91 pKa = 3.26 KK92 pKa = 10.81 RR93 pKa = 11.84 ATAFTVPLSTSWCFFRR109 pKa = 11.84 NLKK112 pKa = 8.79 GHH114 pKa = 6.52 LMEE117 pKa = 5.53 EE118 pKa = 4.25 PLLNLLGEE126 pKa = 4.77 GSWQVGYY133 pKa = 10.42 ILPRR137 pKa = 11.84 RR138 pKa = 11.84 FSMLYY143 pKa = 9.75 EE144 pKa = 4.1 LLNGKK149 pKa = 9.57 LGFNQLLSRR158 pKa = 4.53
Molecular weight: 17.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.677
IPC_protein 10.438
Toseland 10.73
ProMoST 10.643
Dawson 10.818
Bjellqvist 10.526
Wikipedia 11.008
Rodwell 11.052
Grimsley 10.862
Solomon 10.935
Lehninger 10.906
Nozaki 10.73
DTASelect 10.511
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.804
IPC_peptide 10.935
IPC2_peptide 9.736
IPC2.peptide.svr19 8.61
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36118
0
36118
14223905
9
7775
393.8
43.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.661 ± 0.01
1.89 ± 0.006
5.255 ± 0.009
6.473 ± 0.015
4.191 ± 0.008
6.723 ± 0.013
2.393 ± 0.006
5.4 ± 0.01
5.913 ± 0.011
9.735 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.472 ± 0.005
4.478 ± 0.009
4.943 ± 0.014
3.738 ± 0.008
5.292 ± 0.011
8.925 ± 0.016
4.928 ± 0.008
6.51 ± 0.007
1.33 ± 0.005
2.749 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here