Influenza A virus (strain A/Duck/Hokkaido/8/1980 H3N8)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H3N8 subtype

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q08II4|PB1F2_I80A6 Protein PB1-F2 OS=Influenza A virus (strain A/Duck/Hokkaido/8/1980 H3N8) OX=387207 GN=PB1 PE=3 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.4VEE8 pKa = 4.38TPTRR12 pKa = 11.84NGWEE16 pKa = 4.59CKK18 pKa = 10.26CSDD21 pKa = 3.73SSDD24 pKa = 3.78PLVIAASIIGILHH37 pKa = 7.1LILWILDD44 pKa = 3.39RR45 pKa = 11.84LFFKK49 pKa = 10.78CIYY52 pKa = 9.95RR53 pKa = 11.84RR54 pKa = 11.84LKK56 pKa = 10.55YY57 pKa = 10.03GLKK60 pKa = 10.09RR61 pKa = 11.84GPSTEE66 pKa = 4.02GVPEE70 pKa = 4.24SMRR73 pKa = 11.84EE74 pKa = 3.92EE75 pKa = 4.08YY76 pKa = 10.41RR77 pKa = 11.84QEE79 pKa = 3.67QQNAVDD85 pKa = 3.55VDD87 pKa = 3.97DD88 pKa = 4.06GHH90 pKa = 6.42FVNIEE95 pKa = 3.83LEE97 pKa = 4.08

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q08II5|RDRP_I80A6 RNA-directed RNA polymerase catalytic subunit OS=Influenza A virus (strain A/Duck/Hokkaido/8/1980 H3N8) OX=387207 GN=PB1 PE=3 SV=1
MM1 pKa = 7.21EE2 pKa = 4.72QEE4 pKa = 4.99QDD6 pKa = 3.84TPWIQSTEE14 pKa = 3.7HH15 pKa = 5.86TNIQKK20 pKa = 10.2RR21 pKa = 11.84EE22 pKa = 4.12SGQQTQRR29 pKa = 11.84LEE31 pKa = 4.37HH32 pKa = 6.72PSLTRR37 pKa = 11.84LMDD40 pKa = 4.59PYY42 pKa = 10.32PRR44 pKa = 11.84TMNRR48 pKa = 11.84ADD50 pKa = 3.25THH52 pKa = 5.59KK53 pKa = 10.9QIVSWRR59 pKa = 11.84QWLSLKK65 pKa = 10.78NPTQGSLKK73 pKa = 9.64TRR75 pKa = 11.84VSKK78 pKa = 10.39RR79 pKa = 11.84WKK81 pKa = 10.3LFSKK85 pKa = 9.98QEE87 pKa = 3.82WTNN90 pKa = 3.2

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

4808

90

759

400.7

45.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.782 ± 0.4

1.809 ± 0.313

4.908 ± 0.264

7.425 ± 0.732

3.931 ± 0.272

6.531 ± 0.552

1.622 ± 0.12

6.552 ± 0.372

5.761 ± 0.494

7.737 ± 0.472

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.848 ± 0.376

5.075 ± 0.462

3.889 ± 0.177

4.139 ± 0.383

7.009 ± 0.545

7.176 ± 0.205

6.635 ± 0.347

5.865 ± 0.78

1.664 ± 0.19

2.641 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski