Amino acid dipepetide frequency for Bacillus sp. cl95

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.493AlaAla: 5.493 ± 0.108
0.55AlaCys: 0.55 ± 0.024
3.314AlaAsp: 3.314 ± 0.059
4.673AlaGlu: 4.673 ± 0.07
3.276AlaPhe: 3.276 ± 0.064
5.213AlaGly: 5.213 ± 0.085
1.215AlaHis: 1.215 ± 0.035
5.787AlaIle: 5.787 ± 0.083
4.893AlaLys: 4.893 ± 0.082
6.798AlaLeu: 6.798 ± 0.084
2.104AlaMet: 2.104 ± 0.04
2.784AlaAsn: 2.784 ± 0.051
1.953AlaPro: 1.953 ± 0.048
2.088AlaGln: 2.088 ± 0.045
2.51AlaArg: 2.51 ± 0.055
3.945AlaSer: 3.945 ± 0.061
3.428AlaThr: 3.428 ± 0.055
5.142AlaVal: 5.142 ± 0.081
0.556AlaTrp: 0.556 ± 0.024
2.087AlaTyr: 2.087 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
0.412CysAla: 0.412 ± 0.022
0.086CysCys: 0.086 ± 0.009
0.387CysAsp: 0.387 ± 0.019
0.519CysGlu: 0.519 ± 0.025
0.314CysPhe: 0.314 ± 0.017
0.664CysGly: 0.664 ± 0.025
0.206CysHis: 0.206 ± 0.013
0.504CysIle: 0.504 ± 0.024
0.369CysLys: 0.369 ± 0.017
0.667CysLeu: 0.667 ± 0.023
0.178CysMet: 0.178 ± 0.012
0.285CysAsn: 0.285 ± 0.016
0.351CysPro: 0.351 ± 0.021
0.208CysGln: 0.208 ± 0.014
0.285CysArg: 0.285 ± 0.017
0.516CysSer: 0.516 ± 0.018
0.372CysThr: 0.372 ± 0.018
0.415CysVal: 0.415 ± 0.019
0.068CysTrp: 0.068 ± 0.008
0.247CysTyr: 0.247 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
3.175AspAla: 3.175 ± 0.059
0.401AspCys: 0.401 ± 0.02
2.434AspAsp: 2.434 ± 0.048
4.352AspGlu: 4.352 ± 0.068
2.643AspPhe: 2.643 ± 0.048
3.433AspGly: 3.433 ± 0.071
1.152AspHis: 1.152 ± 0.034
3.993AspIle: 3.993 ± 0.064
3.247AspLys: 3.247 ± 0.06
5.087AspLeu: 5.087 ± 0.072
1.359AspMet: 1.359 ± 0.038
1.749AspAsn: 1.749 ± 0.039
1.925AspPro: 1.925 ± 0.044
1.893AspGln: 1.893 ± 0.043
2.218AspArg: 2.218 ± 0.048
2.744AspSer: 2.744 ± 0.063
2.428AspThr: 2.428 ± 0.044
3.668AspVal: 3.668 ± 0.055
0.665AspTrp: 0.665 ± 0.03
2.099AspTyr: 2.099 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
5.196GluAla: 5.196 ± 0.077
0.415GluCys: 0.415 ± 0.02
3.738GluAsp: 3.738 ± 0.068
6.67GluGlu: 6.67 ± 0.103
2.835GluPhe: 2.835 ± 0.058
4.479GluGly: 4.479 ± 0.075
1.482GluHis: 1.482 ± 0.041
5.846GluIle: 5.846 ± 0.087
7.092GluLys: 7.092 ± 0.102
7.218GluLeu: 7.218 ± 0.096
2.525GluMet: 2.525 ± 0.057
3.843GluAsn: 3.843 ± 0.055
1.798GluPro: 1.798 ± 0.042
2.903GluGln: 2.903 ± 0.057
3.355GluArg: 3.355 ± 0.067
3.725GluSer: 3.725 ± 0.062
3.889GluThr: 3.889 ± 0.063
5.181GluVal: 5.181 ± 0.071
0.819GluTrp: 0.819 ± 0.025
2.339GluTyr: 2.339 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.124PheAla: 3.124 ± 0.054
0.347PheCys: 0.347 ± 0.02
2.434PheAsp: 2.434 ± 0.05
3.135PheGlu: 3.135 ± 0.048
2.564PhePhe: 2.564 ± 0.062
3.443PheGly: 3.443 ± 0.066
1.078PheHis: 1.078 ± 0.03
3.847PheIle: 3.847 ± 0.058
2.664PheLys: 2.664 ± 0.05
5.029PheLeu: 5.029 ± 0.08
1.317PheMet: 1.317 ± 0.037
1.879PheAsn: 1.879 ± 0.038
1.736PhePro: 1.736 ± 0.044
1.683PheGln: 1.683 ± 0.038
1.603PheArg: 1.603 ± 0.045
3.419PheSer: 3.419 ± 0.053
2.624PheThr: 2.624 ± 0.056
3.274PheVal: 3.274 ± 0.058
0.509PheTrp: 0.509 ± 0.023
1.91PheTyr: 1.91 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
4.656GlyAla: 4.656 ± 0.08
0.595GlyCys: 0.595 ± 0.024
3.19GlyAsp: 3.19 ± 0.063
4.487GlyGlu: 4.487 ± 0.063
3.619GlyPhe: 3.619 ± 0.054
4.751GlyGly: 4.751 ± 0.086
1.396GlyHis: 1.396 ± 0.038
5.98GlyIle: 5.98 ± 0.089
5.455GlyLys: 5.455 ± 0.082
6.558GlyLeu: 6.558 ± 0.085
2.175GlyMet: 2.175 ± 0.045
2.914GlyAsn: 2.914 ± 0.053
1.645GlyPro: 1.645 ± 0.048
2.173GlyGln: 2.173 ± 0.045
2.604GlyArg: 2.604 ± 0.049
3.987GlySer: 3.987 ± 0.075
3.996GlyThr: 3.996 ± 0.071
5.085GlyVal: 5.085 ± 0.078
0.956GlyTrp: 0.956 ± 0.059
2.776GlyTyr: 2.776 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
1.218HisAla: 1.218 ± 0.03
0.216HisCys: 0.216 ± 0.014
1.062HisAsp: 1.062 ± 0.033
1.487HisGlu: 1.487 ± 0.044
1.16HisPhe: 1.16 ± 0.034
1.376HisGly: 1.376 ± 0.037
0.699HisHis: 0.699 ± 0.025
1.448HisIle: 1.448 ± 0.037
1.138HisLys: 1.138 ± 0.034
2.177HisLeu: 2.177 ± 0.05
0.518HisMet: 0.518 ± 0.02
0.765HisAsn: 0.765 ± 0.029
1.105HisPro: 1.105 ± 0.033
0.805HisGln: 0.805 ± 0.025
0.87HisArg: 0.87 ± 0.027
1.255HisSer: 1.255 ± 0.034
1.001HisThr: 1.001 ± 0.03
1.347HisVal: 1.347 ± 0.039
0.214HisTrp: 0.214 ± 0.014
0.835HisTyr: 0.835 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.684IleAla: 5.684 ± 0.079
0.597IleCys: 0.597 ± 0.022
4.259IleAsp: 4.259 ± 0.068
5.747IleGlu: 5.747 ± 0.079
3.517IlePhe: 3.517 ± 0.067
5.871IleGly: 5.871 ± 0.073
1.697IleHis: 1.697 ± 0.043
5.875IleIle: 5.875 ± 0.092
4.794IleLys: 4.794 ± 0.068
7.525IleLeu: 7.525 ± 0.094
1.869IleMet: 1.869 ± 0.046
3.241IleAsn: 3.241 ± 0.055
3.267IlePro: 3.267 ± 0.056
2.743IleGln: 2.743 ± 0.046
3.135IleArg: 3.135 ± 0.058
5.314IleSer: 5.314 ± 0.074
4.157IleThr: 4.157 ± 0.06
5.627IleVal: 5.627 ± 0.072
0.655IleTrp: 0.655 ± 0.027
2.471IleTyr: 2.471 ± 0.053
0.0IleXaa: 0.0 ± 0.0
Lys
4.782LysAla: 4.782 ± 0.079
0.364LysCys: 0.364 ± 0.018
4.118LysAsp: 4.118 ± 0.069
6.871LysGlu: 6.871 ± 0.095
2.237LysPhe: 2.237 ± 0.042
4.85LysGly: 4.85 ± 0.069
1.335LysHis: 1.335 ± 0.038
5.144LysIle: 5.144 ± 0.077
6.276LysLys: 6.276 ± 0.094
6.353LysLeu: 6.353 ± 0.082
2.483LysMet: 2.483 ± 0.047
3.78LysAsn: 3.78 ± 0.064
2.266LysPro: 2.266 ± 0.044
2.857LysGln: 2.857 ± 0.052
3.207LysArg: 3.207 ± 0.05
3.808LysSer: 3.808 ± 0.062
3.805LysThr: 3.805 ± 0.058
5.031LysVal: 5.031 ± 0.077
0.98LysTrp: 0.98 ± 0.039
2.277LysTyr: 2.277 ± 0.046
0.0LysXaa: 0.0 ± 0.0
Leu
7.084LeuAla: 7.084 ± 0.086
0.661LeuCys: 0.661 ± 0.026
4.823LeuAsp: 4.823 ± 0.073
6.876LeuGlu: 6.876 ± 0.095
5.011LeuPhe: 5.011 ± 0.089
6.472LeuGly: 6.472 ± 0.091
1.907LeuHis: 1.907 ± 0.047
7.291LeuIle: 7.291 ± 0.104
7.274LeuLys: 7.274 ± 0.094
10.171LeuLeu: 10.171 ± 0.139
2.62LeuMet: 2.62 ± 0.05
4.54LeuAsn: 4.54 ± 0.065
3.716LeuPro: 3.716 ± 0.056
3.533LeuGln: 3.533 ± 0.077
3.675LeuArg: 3.675 ± 0.064
6.944LeuSer: 6.944 ± 0.079
5.606LeuThr: 5.606 ± 0.073
6.301LeuVal: 6.301 ± 0.082
0.858LeuTrp: 0.858 ± 0.027
3.077LeuTyr: 3.077 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.103MetAla: 2.103 ± 0.051
0.169MetCys: 0.169 ± 0.013
1.534MetAsp: 1.534 ± 0.041
2.13MetGlu: 2.13 ± 0.048
1.151MetPhe: 1.151 ± 0.03
1.825MetGly: 1.825 ± 0.04
0.471MetHis: 0.471 ± 0.021
2.295MetIle: 2.295 ± 0.045
2.832MetLys: 2.832 ± 0.045
2.612MetLeu: 2.612 ± 0.042
0.928MetMet: 0.928 ± 0.032
1.745MetAsn: 1.745 ± 0.041
1.059MetPro: 1.059 ± 0.031
0.892MetGln: 0.892 ± 0.028
1.127MetArg: 1.127 ± 0.031
1.683MetSer: 1.683 ± 0.043
1.643MetThr: 1.643 ± 0.041
1.854MetVal: 1.854 ± 0.039
0.186MetTrp: 0.186 ± 0.012
0.744MetTyr: 0.744 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.82AsnAla: 2.82 ± 0.055
0.306AsnCys: 0.306 ± 0.02
2.261AsnAsp: 2.261 ± 0.046
3.763AsnGlu: 3.763 ± 0.065
1.782AsnPhe: 1.782 ± 0.041
3.495AsnGly: 3.495 ± 0.069
1.081AsnHis: 1.081 ± 0.037
3.318AsnIle: 3.318 ± 0.053
2.989AsnLys: 2.989 ± 0.05
4.129AsnLeu: 4.129 ± 0.063
1.286AsnMet: 1.286 ± 0.034
1.984AsnAsn: 1.984 ± 0.051
2.184AsnPro: 2.184 ± 0.037
1.884AsnGln: 1.884 ± 0.054
2.038AsnArg: 2.038 ± 0.044
2.371AsnSer: 2.371 ± 0.047
2.091AsnThr: 2.091 ± 0.043
3.109AsnVal: 3.109 ± 0.049
0.522AsnTrp: 0.522 ± 0.021
1.534AsnTyr: 1.534 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.197ProAla: 2.197 ± 0.044
0.212ProCys: 0.212 ± 0.015
1.87ProAsp: 1.87 ± 0.049
2.922ProGlu: 2.922 ± 0.055
2.013ProPhe: 2.013 ± 0.045
2.217ProGly: 2.217 ± 0.049
0.797ProHis: 0.797 ± 0.028
2.864ProIle: 2.864 ± 0.057
2.287ProLys: 2.287 ± 0.045
3.489ProLeu: 3.489 ± 0.052
0.82ProMet: 0.82 ± 0.029
1.683ProAsn: 1.683 ± 0.043
0.937ProPro: 0.937 ± 0.029
1.145ProGln: 1.145 ± 0.043
1.051ProArg: 1.051 ± 0.033
2.18ProSer: 2.18 ± 0.047
1.892ProThr: 1.892 ± 0.054
2.708ProVal: 2.708 ± 0.054
0.365ProTrp: 0.365 ± 0.017
1.357ProTyr: 1.357 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
2.349GlnAla: 2.349 ± 0.055
0.2GlnCys: 0.2 ± 0.014
1.619GlnAsp: 1.619 ± 0.038
2.665GlnGlu: 2.665 ± 0.052
1.635GlnPhe: 1.635 ± 0.039
2.005GlnGly: 2.005 ± 0.044
0.742GlnHis: 0.742 ± 0.025
2.531GlnIle: 2.531 ± 0.051
2.752GlnLys: 2.752 ± 0.054
3.685GlnLeu: 3.685 ± 0.064
1.154GlnMet: 1.154 ± 0.036
1.688GlnAsn: 1.688 ± 0.043
1.163GlnPro: 1.163 ± 0.035
1.607GlnGln: 1.607 ± 0.05
1.41GlnArg: 1.41 ± 0.034
2.187GlnSer: 2.187 ± 0.042
1.937GlnThr: 1.937 ± 0.041
2.211GlnVal: 2.211 ± 0.05
0.36GlnTrp: 0.36 ± 0.017
1.324GlnTyr: 1.324 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.328ArgAla: 2.328 ± 0.051
0.26ArgCys: 0.26 ± 0.016
2.034ArgAsp: 2.034 ± 0.044
3.155ArgGlu: 3.155 ± 0.065
1.927ArgPhe: 1.927 ± 0.045
2.304ArgGly: 2.304 ± 0.053
0.765ArgHis: 0.765 ± 0.026
3.089ArgIle: 3.089 ± 0.059
3.265ArgLys: 3.265 ± 0.058
3.903ArgLeu: 3.903 ± 0.061
1.297ArgMet: 1.297 ± 0.037
1.994ArgAsn: 1.994 ± 0.037
1.269ArgPro: 1.269 ± 0.033
1.406ArgGln: 1.406 ± 0.042
1.771ArgArg: 1.771 ± 0.049
2.149ArgSer: 2.149 ± 0.043
1.934ArgThr: 1.934 ± 0.039
2.559ArgVal: 2.559 ± 0.048
0.392ArgTrp: 0.392 ± 0.02
1.421ArgTyr: 1.421 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
3.753SerAla: 3.753 ± 0.059
0.42SerCys: 0.42 ± 0.018
3.024SerAsp: 3.024 ± 0.053
4.214SerGlu: 4.214 ± 0.064
3.42SerPhe: 3.42 ± 0.057
4.525SerGly: 4.525 ± 0.082
1.268SerHis: 1.268 ± 0.034
5.044SerIle: 5.044 ± 0.067
4.133SerLys: 4.133 ± 0.068
6.351SerLeu: 6.351 ± 0.086
1.779SerMet: 1.779 ± 0.044
2.588SerAsn: 2.588 ± 0.053
2.094SerPro: 2.094 ± 0.047
2.041SerGln: 2.041 ± 0.039
2.145SerArg: 2.145 ± 0.046
4.015SerSer: 4.015 ± 0.074
3.086SerThr: 3.086 ± 0.06
4.278SerVal: 4.278 ± 0.065
0.609SerTrp: 0.609 ± 0.024
2.145SerTyr: 2.145 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
3.834ThrAla: 3.834 ± 0.074
0.319ThrCys: 0.319 ± 0.017
2.63ThrAsp: 2.63 ± 0.054
3.574ThrGlu: 3.574 ± 0.055
2.633ThrPhe: 2.633 ± 0.049
4.278ThrGly: 4.278 ± 0.07
1.031ThrHis: 1.031 ± 0.03
4.391ThrIle: 4.391 ± 0.062
3.412ThrLys: 3.412 ± 0.054
5.173ThrLeu: 5.173 ± 0.066
1.362ThrMet: 1.362 ± 0.036
2.428ThrAsn: 2.428 ± 0.045
2.178ThrPro: 2.178 ± 0.046
1.319ThrGln: 1.319 ± 0.037
1.728ThrArg: 1.728 ± 0.04
3.276ThrSer: 3.276 ± 0.059
2.944ThrThr: 2.944 ± 0.067
4.094ThrVal: 4.094 ± 0.073
0.523ThrTrp: 0.523 ± 0.027
1.912ThrTyr: 1.912 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
4.955ValAla: 4.955 ± 0.084
0.58ValCys: 0.58 ± 0.027
3.488ValAsp: 3.488 ± 0.058
4.884ValGlu: 4.884 ± 0.071
3.256ValPhe: 3.256 ± 0.06
4.667ValGly: 4.667 ± 0.072
1.308ValHis: 1.308 ± 0.035
5.557ValIle: 5.557 ± 0.077
5.065ValLys: 5.065 ± 0.064
6.77ValLeu: 6.77 ± 0.098
1.935ValMet: 1.935 ± 0.047
3.161ValAsn: 3.161 ± 0.057
2.747ValPro: 2.747 ± 0.05
2.293ValGln: 2.293 ± 0.047
2.565ValArg: 2.565 ± 0.051
4.658ValSer: 4.658 ± 0.068
3.999ValThr: 3.999 ± 0.058
5.053ValVal: 5.053 ± 0.091
0.647ValTrp: 0.647 ± 0.027
2.276ValTyr: 2.276 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.589TrpAla: 0.589 ± 0.02
0.074TrpCys: 0.074 ± 0.008
0.512TrpAsp: 0.512 ± 0.021
0.671TrpGlu: 0.671 ± 0.028
0.552TrpPhe: 0.552 ± 0.023
0.698TrpGly: 0.698 ± 0.027
0.202TrpHis: 0.202 ± 0.013
0.871TrpIle: 0.871 ± 0.031
0.804TrpLys: 0.804 ± 0.027
1.173TrpLeu: 1.173 ± 0.035
0.369TrpMet: 0.369 ± 0.018
0.551TrpAsn: 0.551 ± 0.024
0.221TrpPro: 0.221 ± 0.016
0.353TrpGln: 0.353 ± 0.015
0.36TrpArg: 0.36 ± 0.017
0.589TrpSer: 0.589 ± 0.025
0.525TrpThr: 0.525 ± 0.021
0.7TrpVal: 0.7 ± 0.027
0.142TrpTrp: 0.142 ± 0.012
0.486TrpTyr: 0.486 ± 0.039
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.058TyrAla: 2.058 ± 0.049
0.283TyrCys: 0.283 ± 0.016
1.933TyrAsp: 1.933 ± 0.045
2.555TyrGlu: 2.555 ± 0.05
2.073TyrPhe: 2.073 ± 0.046
2.432TyrGly: 2.432 ± 0.053
0.864TyrHis: 0.864 ± 0.026
2.371TyrIle: 2.371 ± 0.05
2.082TyrLys: 2.082 ± 0.043
3.571TyrLeu: 3.571 ± 0.064
0.904TyrMet: 0.904 ± 0.03
1.345TyrAsn: 1.345 ± 0.041
1.393TyrPro: 1.393 ± 0.033
1.397TyrGln: 1.397 ± 0.037
1.563TyrArg: 1.563 ± 0.039
2.167TyrSer: 2.167 ± 0.053
1.682TyrThr: 1.682 ± 0.044
2.228TyrVal: 2.228 ± 0.048
0.415TyrTrp: 0.415 ± 0.02
1.525TyrTyr: 1.525 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3914 proteins (1110460 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski