Faeces associated gemycircularvirus 17
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160HWK6|A0A160HWK6_9VIRU Capsid protein OS=Faeces associated gemycircularvirus 17 OX=1843737 PE=4 SV=1
MM1 pKa = 7.81 PATSLLHH8 pKa = 5.51 TRR10 pKa = 11.84 SAVLLMSGPLTTILDD25 pKa = 3.9 LLEE28 pKa = 5.63 RR29 pKa = 11.84 SVSWDD34 pKa = 3.3 EE35 pKa = 4.64 KK36 pKa = 10.16 FMLMEE41 pKa = 4.04 VLISMFSAISAEE53 pKa = 4.18 SFDD56 pKa = 3.92 PDD58 pKa = 3.41 EE59 pKa = 5.69 PISLMCRR66 pKa = 11.84 VITQTSSDD74 pKa = 3.44 LKK76 pKa = 9.38 EE77 pKa = 3.83 TLEE80 pKa = 4.21 RR81 pKa = 11.84 VRR83 pKa = 11.84 VTQQKK88 pKa = 8.83 MEE90 pKa = 4.28 TLSPVGSPWNPCPLRR105 pKa = 11.84 SFQALKK111 pKa = 10.52 IRR113 pKa = 11.84 GLRR116 pKa = 11.84 SSAAKK121 pKa = 10.15 VEE123 pKa = 4.38 RR124 pKa = 11.84 NFMRR128 pKa = 11.84 LLTTSVRR135 pKa = 11.84 GISSPDD141 pKa = 3.12 LEE143 pKa = 5.4 AFTHH147 pKa = 5.78 TPSGSGLRR155 pKa = 11.84 SAWATNVPPGSCLAMEE171 pKa = 6.05 LFLTWLATEE180 pKa = 4.46 RR181 pKa = 11.84 EE182 pKa = 3.81 FWMYY186 pKa = 10.13 EE187 pKa = 3.59 VSGSFGRR194 pKa = 11.84 VKK196 pKa = 10.09 TLVIYY201 pKa = 9.06 GPPLTGKK208 pKa = 6.25 TTWAQSLGNHH218 pKa = 5.91 VYY220 pKa = 9.37 MKK222 pKa = 9.72 KK223 pKa = 8.63 TFNAKK228 pKa = 9.31 EE229 pKa = 3.8 ASLCEE234 pKa = 4.24 SKK236 pKa = 10.65 DD237 pKa = 3.31 YY238 pKa = 11.35 CVIDD242 pKa = 5.38 DD243 pKa = 3.72 ISGGIKK249 pKa = 10.12 FFPHH253 pKa = 5.15 WKK255 pKa = 9.73 DD256 pKa = 2.82 WFGGQPYY263 pKa = 9.56 VQIRR267 pKa = 11.84 LLYY270 pKa = 10.03 RR271 pKa = 11.84 DD272 pKa = 3.99 EE273 pKa = 5.47 LLLRR277 pKa = 11.84 WGKK280 pKa = 7.53 PTIWLNNRR288 pKa = 11.84 DD289 pKa = 3.78 PRR291 pKa = 11.84 DD292 pKa = 3.39 QLRR295 pKa = 11.84 DD296 pKa = 3.56 MVGRR300 pKa = 11.84 DD301 pKa = 3.72 YY302 pKa = 11.65 SDD304 pKa = 3.39 EE305 pKa = 4.01 QYY307 pKa = 11.48 QNDD310 pKa = 4.55 VEE312 pKa = 4.8 WMDD315 pKa = 3.81 ANCIFVYY322 pKa = 10.21 VDD324 pKa = 3.3 SPIVTFHH331 pKa = 7.16 ANTEE335 pKa = 4.1
Molecular weight: 38.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.571
IPC2_protein 5.626
IPC_protein 5.614
Toseland 5.817
ProMoST 5.703
Dawson 5.779
Bjellqvist 5.842
Wikipedia 5.741
Rodwell 5.741
Grimsley 5.893
Solomon 5.779
Lehninger 5.753
Nozaki 5.995
DTASelect 6.173
Thurlkill 6.097
EMBOSS 6.059
Sillero 6.084
Patrickios 4.24
IPC_peptide 5.792
IPC2_peptide 6.084
IPC2.peptide.svr19 6.119
Protein with the highest isoelectric point:
>tr|A0A168MG03|A0A168MG03_9VIRU RepA OS=Faeces associated gemycircularvirus 17 OX=1843737 PE=4 SV=1
MM1 pKa = 7.91 PYY3 pKa = 10.17 QRR5 pKa = 11.84 RR6 pKa = 11.84 SYY8 pKa = 10.42 SSRR11 pKa = 11.84 ARR13 pKa = 11.84 RR14 pKa = 11.84 PTYY17 pKa = 9.22 RR18 pKa = 11.84 TARR21 pKa = 11.84 PARR24 pKa = 11.84 YY25 pKa = 7.18 TRR27 pKa = 11.84 NSYY30 pKa = 8.89 SRR32 pKa = 11.84 RR33 pKa = 11.84 PTSRR37 pKa = 11.84 RR38 pKa = 11.84 TPIRR42 pKa = 11.84 RR43 pKa = 11.84 TYY45 pKa = 10.43 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 STEE51 pKa = 3.11 KK52 pKa = 7.8 MPRR55 pKa = 11.84 YY56 pKa = 8.51 RR57 pKa = 11.84 RR58 pKa = 11.84 SYY60 pKa = 9.28 RR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 PMTRR68 pKa = 11.84 KK69 pKa = 10.06 KK70 pKa = 10.2 ILNLTSVKK78 pKa = 10.53 KK79 pKa = 9.9 QDD81 pKa = 3.56 NMQLSSNVTAATPQGSATYY100 pKa = 8.61 TNSAAVLPATQLYY113 pKa = 8.64 IFPWIATARR122 pKa = 11.84 DD123 pKa = 3.37 LDD125 pKa = 4.07 RR126 pKa = 11.84 DD127 pKa = 3.64 TGNIRR132 pKa = 11.84 GAPSDD137 pKa = 3.69 SATRR141 pKa = 11.84 TAQTCYY147 pKa = 9.91 MVGLRR152 pKa = 11.84 EE153 pKa = 4.26 NIEE156 pKa = 3.65 ITTNDD161 pKa = 3.41 GLPWQWRR168 pKa = 11.84 RR169 pKa = 11.84 ICFTFKK175 pKa = 10.65 GSEE178 pKa = 4.09 FTATSTTGLNYY189 pKa = 10.39 YY190 pKa = 9.97 LQTSNGMTRR199 pKa = 11.84 TVNTAYY205 pKa = 10.44 GSTAVGVLQSLLFKK219 pKa = 10.76 GAAGVDD225 pKa = 3.33 WSNTITAPTDD235 pKa = 3.09 NTTISVKK242 pKa = 9.75 YY243 pKa = 10.31 DD244 pKa = 3.24 RR245 pKa = 11.84 TISIASGNEE254 pKa = 3.19 QGTIRR259 pKa = 11.84 RR260 pKa = 11.84 YY261 pKa = 10.17 RR262 pKa = 11.84 RR263 pKa = 11.84 FHH265 pKa = 6.47 PMGHH269 pKa = 6.24 NIIYY273 pKa = 10.52 ADD275 pKa = 4.11 DD276 pKa = 3.96 EE277 pKa = 4.51 QGGTEE282 pKa = 4.07 SEE284 pKa = 4.59 SFVSSRR290 pKa = 11.84 GKK292 pKa = 9.8 PGMGDD297 pKa = 3.54 YY298 pKa = 10.92 YY299 pKa = 11.12 VIDD302 pKa = 3.42 IFQPRR307 pKa = 11.84 FGGTSASQLRR317 pKa = 11.84 FEE319 pKa = 5.14 PSATLYY325 pKa = 9.32 WHH327 pKa = 6.99 EE328 pKa = 4.35 KK329 pKa = 9.3
Molecular weight: 37.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 10.043
IPC_protein 10.965
Toseland 10.628
ProMoST 10.599
Dawson 10.804
Bjellqvist 10.628
Wikipedia 11.111
Rodwell 10.818
Grimsley 10.906
Solomon 10.95
Lehninger 10.891
Nozaki 10.613
DTASelect 10.628
Thurlkill 10.687
EMBOSS 11.067
Sillero 10.745
Patrickios 10.379
IPC_peptide 10.95
IPC2_peptide 9.765
IPC2.peptide.svr19 8.143
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
916
252
335
305.3
34.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.441 ± 0.623
1.419 ± 0.36
4.258 ± 0.669
5.022 ± 0.879
3.712 ± 0.376
6.004 ± 0.341
1.31 ± 0.177
4.476 ± 0.351
3.275 ± 0.422
9.061 ± 2.011
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.384 ± 0.423
3.275 ± 0.594
5.895 ± 0.438
2.948 ± 0.519
8.952 ± 1.748
10.917 ± 1.263
9.061 ± 1.514
4.803 ± 0.759
2.511 ± 0.466
3.275 ± 1.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here