Streptomyces phage Raleigh

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Raleighvirus; Streptomyces virus Raleigh

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MCS6|A0A1J0MCS6_9CAUD Uncharacterized protein OS=Streptomyces phage Raleigh OX=1920312 GN=SEA_RALEIGH_47 PE=4 SV=1
MM1 pKa = 7.05FVEE4 pKa = 4.97FFPVVVTALPADD16 pKa = 4.35EE17 pKa = 5.28DD18 pKa = 4.14HH19 pKa = 7.31APLLVDD25 pKa = 3.83PAAARR30 pKa = 11.84IVRR33 pKa = 11.84AEE35 pKa = 3.85QVVEE39 pKa = 3.91GDD41 pKa = 3.49TVLASFVAYY50 pKa = 9.72GEE52 pKa = 4.2RR53 pKa = 11.84MPAADD58 pKa = 4.51YY59 pKa = 11.29FNDD62 pKa = 3.47QYY64 pKa = 10.58TARR67 pKa = 11.84PKK69 pKa = 10.58PYY71 pKa = 10.26DD72 pKa = 3.62PTCGCGSCATMTDD85 pKa = 3.16HH86 pKa = 7.17EE87 pKa = 5.24GPVVDD92 pKa = 6.13LGDD95 pKa = 4.33DD96 pKa = 4.07NPWDD100 pKa = 3.87VCDD103 pKa = 4.17PWPAADD109 pKa = 4.7LVLIVPAAA117 pKa = 3.53

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0MCU9|A0A1J0MCU9_9CAUD Uncharacterized protein OS=Streptomyces phage Raleigh OX=1920312 GN=SEA_RALEIGH_12 PE=4 SV=1
MM1 pKa = 7.61GNNNSTDD8 pKa = 3.04RR9 pKa = 11.84RR10 pKa = 11.84PAKK13 pKa = 10.37KK14 pKa = 10.02SGPPAAGDD22 pKa = 3.65RR23 pKa = 11.84PSLRR27 pKa = 11.84IDD29 pKa = 4.11DD30 pKa = 4.49EE31 pKa = 4.41LAADD35 pKa = 4.03LAEE38 pKa = 4.16IMRR41 pKa = 11.84VHH43 pKa = 6.5PTFADD48 pKa = 3.48AVRR51 pKa = 11.84RR52 pKa = 11.84AVGQLATQYY61 pKa = 8.79RR62 pKa = 11.84TAWANNVCPSDD73 pKa = 3.61TAPVLLAYY81 pKa = 10.48QLTNPATLRR90 pKa = 11.84RR91 pKa = 11.84TPPVARR97 pKa = 11.84RR98 pKa = 11.84MPPPPPSEE106 pKa = 3.66QLRR109 pKa = 11.84PVFSEE114 pKa = 4.08TLLGGTPPPARR125 pKa = 11.84PDD127 pKa = 3.28AQRR130 pKa = 11.84PARR133 pKa = 11.84TT134 pKa = 3.51

Molecular weight: 14.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12891

53

802

243.2

26.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.15 ± 0.495

0.745 ± 0.148

7.145 ± 0.326

5.709 ± 0.4

1.97 ± 0.132

8.719 ± 0.468

2.04 ± 0.171

3.018 ± 0.236

2.304 ± 0.32

8.362 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.823 ± 0.165

2.11 ± 0.164

6.346 ± 0.393

3.91 ± 0.319

8.913 ± 0.549

4.515 ± 0.226

6.252 ± 0.316

7.013 ± 0.314

1.908 ± 0.131

2.048 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski