Streptococcus satellite phage Javan276
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZMW0|A0A4D5ZMW0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan276 OX=2558600 GN=JavanS276_0015 PE=4 SV=1
MM1 pKa = 7.13 YY2 pKa = 10.29 NEE4 pKa = 5.34 EE5 pKa = 4.08 IAKK8 pKa = 9.52 MILSFKK14 pKa = 10.68 KK15 pKa = 10.12 DD16 pKa = 3.11 DD17 pKa = 3.97 CRR19 pKa = 11.84 PEE21 pKa = 4.5 EE22 pKa = 4.02 FDD24 pKa = 3.64 EE25 pKa = 6.11 DD26 pKa = 4.71 DD27 pKa = 4.61 FIEE30 pKa = 4.35 TARR33 pKa = 11.84 QLIASGQIEE42 pKa = 4.83 GKK44 pKa = 10.59 LKK46 pKa = 10.66 LDD48 pKa = 3.62 YY49 pKa = 10.85 LSPYY53 pKa = 10.59 LDD55 pKa = 4.29 LLYY58 pKa = 10.6 QKK60 pKa = 10.15 QHH62 pKa = 4.59 TT63 pKa = 4.03
Molecular weight: 7.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.529
IPC2_protein 4.558
IPC_protein 4.418
Toseland 4.253
ProMoST 4.546
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.266
Rodwell 4.253
Grimsley 4.164
Solomon 4.368
Lehninger 4.317
Nozaki 4.495
DTASelect 4.66
Thurlkill 4.279
EMBOSS 4.279
Sillero 4.533
Patrickios 3.757
IPC_peptide 4.368
IPC2_peptide 4.52
IPC2.peptide.svr19 4.48
Protein with the highest isoelectric point:
>tr|A0A4D5ZI93|A0A4D5ZI93_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan276 OX=2558600 GN=JavanS276_0017 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.56 LFNKK6 pKa = 9.74 KK7 pKa = 10.24 CLVCGTRR14 pKa = 11.84 FKK16 pKa = 10.3 TQSNVSKK23 pKa = 10.74 YY24 pKa = 10.81 CSDD27 pKa = 3.46 NCRR30 pKa = 11.84 KK31 pKa = 9.91 QSVKK35 pKa = 10.11 EE36 pKa = 3.89 RR37 pKa = 11.84 QKK39 pKa = 11.09 QFMKK43 pKa = 10.53 EE44 pKa = 3.45 KK45 pKa = 9.6 RR46 pKa = 11.84 KK47 pKa = 10.12 KK48 pKa = 9.64 IKK50 pKa = 9.89 EE51 pKa = 3.95 EE52 pKa = 3.63 KK53 pKa = 10.0 SINRR57 pKa = 11.84 RR58 pKa = 11.84 SFTNTDD64 pKa = 3.5 FKK66 pKa = 10.93 KK67 pKa = 10.7 APKK70 pKa = 9.16 IDD72 pKa = 3.84 YY73 pKa = 9.62 FDD75 pKa = 6.01 PIQKK79 pKa = 10.17 KK80 pKa = 7.5 MNKK83 pKa = 9.1 ALKK86 pKa = 10.15 IKK88 pKa = 10.27 DD89 pKa = 3.38 FKK91 pKa = 10.82 TYY93 pKa = 10.68 YY94 pKa = 10.08 EE95 pKa = 4.36 LYY97 pKa = 10.08 KK98 pKa = 10.65 KK99 pKa = 10.58 RR100 pKa = 11.84 ILDD103 pKa = 4.21 NEE105 pKa = 4.41 AEE107 pKa = 3.93 WGIVGRR113 pKa = 11.84 HH114 pKa = 4.7 IVNGIEE120 pKa = 3.91 VHH122 pKa = 7.24 DD123 pKa = 4.27 SDD125 pKa = 5.09 FVTMVLKK132 pKa = 10.03 TIDD135 pKa = 3.2 QQ136 pKa = 3.74
Molecular weight: 16.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.989
IPC2_protein 9.151
IPC_protein 9.019
Toseland 10.116
ProMoST 9.619
Dawson 10.233
Bjellqvist 9.823
Wikipedia 10.321
Rodwell 10.965
Grimsley 10.262
Solomon 10.248
Lehninger 10.233
Nozaki 10.116
DTASelect 9.809
Thurlkill 10.101
EMBOSS 10.482
Sillero 10.145
Patrickios 10.672
IPC_peptide 10.248
IPC2_peptide 8.331
IPC2.peptide.svr19 8.085
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
2995
38
376
166.4
19.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.84 ± 0.439
0.801 ± 0.29
6.21 ± 0.364
8.447 ± 0.554
5.008 ± 0.585
3.639 ± 0.436
1.937 ± 0.248
8.715 ± 0.731
10.718 ± 0.679
9.316 ± 0.619
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.27 ± 0.332
7.279 ± 0.461
2.104 ± 0.315
4.107 ± 0.454
4.307 ± 0.466
6.678 ± 0.559
4.775 ± 0.31
4.608 ± 0.537
1.002 ± 0.287
4.24 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here