Amphibalanus amphitrite (Striped barnacle) (Balanus amphitrite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Crustacea; Multicrustacea; Hexanauplia; Thecostraca; Cirripedia; Thoracica; Sessilia; Balanidae; Amphibalanus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A4WIG3|A0A6A4WIG3_AMPAM Kelch-like protein 20 OS=Amphibalanus amphitrite OX=1232801 GN=Klhl20 PE=4 SV=1
MM1 pKa = 7.37RR2 pKa = 11.84RR3 pKa = 11.84CVGRR7 pKa = 11.84TLRR10 pKa = 11.84RR11 pKa = 11.84YY12 pKa = 8.3GTDD15 pKa = 2.91TTLEE19 pKa = 4.7DD20 pKa = 4.29PSQSDD25 pKa = 3.66DD26 pKa = 3.25VTDD29 pKa = 4.51RR30 pKa = 11.84VTEE33 pKa = 4.16DD34 pKa = 3.03LTDD37 pKa = 3.88GVVSGSVSEE46 pKa = 4.39QAWDD50 pKa = 4.06PYY52 pKa = 10.32QASSDD57 pKa = 3.6SDD59 pKa = 4.04SVSEE63 pKa = 4.26QAWDD67 pKa = 4.06PYY69 pKa = 10.33QASSDD74 pKa = 3.72SGSVSEE80 pKa = 4.75QAWDD84 pKa = 3.99PYY86 pKa = 10.12QASSASGSVSEE97 pKa = 4.66QAWDD101 pKa = 3.99PYY103 pKa = 10.12QASSASGSVSEE114 pKa = 4.66QAWDD118 pKa = 3.99PYY120 pKa = 10.12QASSASGSVSGQAWDD135 pKa = 4.42PYY137 pKa = 10.12QASSDD142 pKa = 3.72SGSVSEE148 pKa = 4.75QAWDD152 pKa = 3.99PYY154 pKa = 10.12QASSASGSVSEE165 pKa = 4.66QAWDD169 pKa = 4.06PYY171 pKa = 10.32QASSDD176 pKa = 3.6SDD178 pKa = 4.04SVSEE182 pKa = 4.26QAWDD186 pKa = 4.06PYY188 pKa = 10.33QASSDD193 pKa = 3.72SGSVSEE199 pKa = 4.75QAWDD203 pKa = 3.99PYY205 pKa = 10.12QASSASGSVSEE216 pKa = 4.66QAWDD220 pKa = 3.99PYY222 pKa = 10.12QASSASGSVSEE233 pKa = 4.66QAWDD237 pKa = 3.99PYY239 pKa = 10.12QASSASGSVSGQAWDD254 pKa = 4.42PYY256 pKa = 10.12QASSDD261 pKa = 3.72SGSVSEE267 pKa = 4.75QAWDD271 pKa = 3.99PYY273 pKa = 10.03QASSASDD280 pKa = 3.58SVSEE284 pKa = 4.23QAWDD288 pKa = 3.99PYY290 pKa = 10.03QASSASDD297 pKa = 3.58SVSEE301 pKa = 4.23QAWDD305 pKa = 4.06PYY307 pKa = 10.33QASSDD312 pKa = 3.72SGSVSEE318 pKa = 4.75QAWDD322 pKa = 4.06PYY324 pKa = 10.32QASSDD329 pKa = 3.89SDD331 pKa = 3.53SQEE334 pKa = 3.84YY335 pKa = 10.55KK336 pKa = 10.42EE337 pKa = 4.12VSEE340 pKa = 4.92PGSEE344 pKa = 3.91PAVDD348 pKa = 3.83PEE350 pKa = 4.11TLRR353 pKa = 11.84RR354 pKa = 11.84FLDD357 pKa = 3.4TDD359 pKa = 3.81DD360 pKa = 3.65YY361 pKa = 11.83RR362 pKa = 11.84NFIDD366 pKa = 4.96SLSDD370 pKa = 3.46CTSSQVSGAPARR382 pKa = 11.84PPPPRR387 pKa = 11.84RR388 pKa = 11.84QRR390 pKa = 11.84RR391 pKa = 11.84KK392 pKa = 9.88LRR394 pKa = 11.84AAASDD399 pKa = 4.01SVDD402 pKa = 3.24SDD404 pKa = 3.49TDD406 pKa = 3.52GGDD409 pKa = 3.24EE410 pKa = 3.9VAAALVEE417 pKa = 4.38SRR419 pKa = 11.84RR420 pKa = 11.84RR421 pKa = 11.84LQLARR426 pKa = 11.84TEE428 pKa = 4.21LSRR431 pKa = 11.84RR432 pKa = 11.84AHH434 pKa = 6.1QPDD437 pKa = 5.33PICVSNGYY445 pKa = 9.1RR446 pKa = 11.84VPAGPQYY453 pKa = 10.27RR454 pKa = 11.84VPAGPHH460 pKa = 5.35LCVV463 pKa = 3.59

Molecular weight: 49.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A4X3Q4|A0A6A4X3Q4_AMPAM Uncharacterized protein OS=Amphibalanus amphitrite OX=1232801 GN=FJT64_019337 PE=4 SV=1
MM1 pKa = 7.23IRR3 pKa = 11.84WPQTALVRR11 pKa = 11.84QTALVRR17 pKa = 11.84QTALVRR23 pKa = 11.84RR24 pKa = 11.84TTLVRR29 pKa = 11.84QTALVRR35 pKa = 11.84RR36 pKa = 11.84TALVRR41 pKa = 11.84RR42 pKa = 11.84TALVRR47 pKa = 11.84QTALVRR53 pKa = 11.84RR54 pKa = 11.84TALVRR59 pKa = 11.84RR60 pKa = 11.84TALVRR65 pKa = 11.84QTALVRR71 pKa = 11.84QTALVRR77 pKa = 11.84QTVLVRR83 pKa = 11.84QTALVRR89 pKa = 11.84QTVLVRR95 pKa = 11.84QTALVRR101 pKa = 11.84QTALVRR107 pKa = 11.84RR108 pKa = 11.84TALVRR113 pKa = 11.84QTALVRR119 pKa = 11.84RR120 pKa = 11.84TALVRR125 pKa = 11.84RR126 pKa = 11.84TALVRR131 pKa = 11.84RR132 pKa = 11.84TALVRR137 pKa = 11.84QTALVRR143 pKa = 11.84QTALVRR149 pKa = 11.84QTALVRR155 pKa = 11.84QTALVRR161 pKa = 11.84QTALVRR167 pKa = 11.84RR168 pKa = 11.84TALVRR173 pKa = 11.84QTALVRR179 pKa = 11.84QTALVRR185 pKa = 11.84RR186 pKa = 11.84TALVRR191 pKa = 11.84QTALVRR197 pKa = 11.84RR198 pKa = 11.84TALVRR203 pKa = 11.84RR204 pKa = 11.84TALVRR209 pKa = 11.84RR210 pKa = 11.84TALVRR215 pKa = 11.84RR216 pKa = 11.84TALVRR221 pKa = 11.84RR222 pKa = 11.84TALVRR227 pKa = 11.84QTALMWMLATLVALDD242 pKa = 4.72PGHH245 pKa = 6.57SGWCFHH251 pKa = 7.08PLLSRR256 pKa = 11.84QMASEE261 pKa = 3.8ILVPDD266 pKa = 4.5RR267 pKa = 11.84MAGWKK272 pKa = 10.23VNN274 pKa = 3.41

Molecular weight: 31.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24795

1364

26159

11229583

50

8844

429.3

47.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.954 ± 0.023

2.06 ± 0.016

5.489 ± 0.012

6.304 ± 0.019

2.943 ± 0.01

7.306 ± 0.019

2.41 ± 0.007

3.143 ± 0.012

3.598 ± 0.016

9.582 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.032 ± 0.007

2.586 ± 0.01

6.753 ± 0.021

4.358 ± 0.013

7.903 ± 0.017

7.435 ± 0.017

5.583 ± 0.012

6.853 ± 0.016

1.3 ± 0.006

2.407 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski