Dishui Lake virophage 7
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G6XMZ1|A0A6G6XMZ1_9VIRU Uncharacterized protein OS=Dishui Lake virophage 7 OX=2704070 PE=4 SV=1
MM1 pKa = 7.24 SVSSILNPATGKK13 pKa = 10.33 IYY15 pKa = 10.61 DD16 pKa = 4.26 DD17 pKa = 4.94 LVPQGGGVPLAKK29 pKa = 10.36 GGLITANAGGAEE41 pKa = 3.98 QALAVGNDD49 pKa = 3.12 GWVLSADD56 pKa = 3.74 SNEE59 pKa = 3.98 ALGLKK64 pKa = 9.55 YY65 pKa = 10.16 IQLPAGGIQFTQQGQLLYY83 pKa = 10.68 AGAGPAFNDD92 pKa = 3.3 SLLNIGNAGQILGINAGIPAWINAGGSGTITALAPLTEE130 pKa = 4.45 YY131 pKa = 11.54 ADD133 pKa = 3.79 GTASKK138 pKa = 10.69 VAVNFTGKK146 pKa = 10.69 GDD148 pKa = 4.61 LIVGGGVQVGGEE160 pKa = 4.03 PVAGVILPVGANDD173 pKa = 3.57 MVLTANSATASGLEE187 pKa = 4.12 WKK189 pKa = 10.59 ASGGGSSATIFRR201 pKa = 11.84 NSVDD205 pKa = 3.72 DD206 pKa = 3.94 TPLVITKK213 pKa = 9.1 PATANDD219 pKa = 3.93 TCVITADD226 pKa = 3.99 RR227 pKa = 11.84 TYY229 pKa = 10.78 HH230 pKa = 7.0 AYY232 pKa = 8.06 NTQIYY237 pKa = 9.45 SKK239 pKa = 10.37 GGVGTQVDD247 pKa = 4.09 MTNPQPFPFFTWTAPNDD264 pKa = 3.38 INMTSVSSQVKK275 pKa = 9.99 IITNSIYY282 pKa = 10.65 GQTVHH287 pKa = 7.89 LDD289 pKa = 3.3 IYY291 pKa = 10.97 DD292 pKa = 3.81 STGTTGLVGGDD303 pKa = 3.66 PVNVSDD309 pKa = 5.68 NEE311 pKa = 4.15 PAGDD315 pKa = 3.82 AVSLPITTAFQFVNGTTYY333 pKa = 11.16 QFMVSLDD340 pKa = 3.69 GLDD343 pKa = 3.16 QGEE346 pKa = 4.35 FFDD349 pKa = 4.19 MFIDD353 pKa = 4.56 GGQYY357 pKa = 10.2 VGNITINGVDD367 pKa = 4.11 YY368 pKa = 11.45 ANLPATFTLAPPYY381 pKa = 10.06 KK382 pKa = 10.26 FRR384 pKa = 11.84 VSNDD388 pKa = 3.18 LTGKK392 pKa = 8.94 TSATCEE398 pKa = 4.13 NFSSQSFVASEE409 pKa = 4.52 DD410 pKa = 3.83 TNSWIAVGGINAGVAFVPP428 pKa = 4.51
Molecular weight: 43.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.91
IPC_protein 3.923
Toseland 3.694
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.745
Grimsley 3.605
Solomon 3.923
Lehninger 3.884
Nozaki 4.037
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.897
Sillero 4.05
Patrickios 1.939
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|A0A6G6XMY2|A0A6G6XMY2_9VIRU Uncharacterized protein OS=Dishui Lake virophage 7 OX=2704070 PE=4 SV=1
MM1 pKa = 7.29 LQEE4 pKa = 4.31 NNNNSFMLCSYY15 pKa = 10.05 SKK17 pKa = 10.87 KK18 pKa = 10.55 SFSATLLSRR27 pKa = 11.84 DD28 pKa = 3.44 FSTASSSFFRR38 pKa = 11.84 SITAFCSSARR48 pKa = 11.84 FLIISLNASSCVGSAFRR65 pKa = 11.84 FFQNFNHH72 pKa = 6.64 SGRR75 pKa = 11.84 KK76 pKa = 5.61 YY77 pKa = 10.32 HH78 pKa = 7.04 IYY80 pKa = 10.91 LFII83 pKa = 6.13
Molecular weight: 9.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.238
IPC2_protein 9.297
IPC_protein 9.472
Toseland 9.692
ProMoST 9.56
Dawson 9.999
Bjellqvist 9.809
Wikipedia 10.204
Rodwell 10.175
Grimsley 10.072
Solomon 10.072
Lehninger 10.043
Nozaki 9.926
DTASelect 9.75
Thurlkill 9.838
EMBOSS 10.131
Sillero 9.97
Patrickios 7.468
IPC_peptide 10.072
IPC2_peptide 9.107
IPC2.peptide.svr19 7.955
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27
0
27
8892
80
977
329.3
36.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.872 ± 0.347
1.181 ± 0.188
5.578 ± 0.345
5.87 ± 0.74
3.936 ± 0.222
8.221 ± 1.124
1.203 ± 0.184
5.713 ± 0.349
6.984 ± 0.808
7.94 ± 0.334
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.878 ± 0.181
5.567 ± 0.294
4.915 ± 0.417
4.926 ± 0.426
4.251 ± 0.55
7.175 ± 0.544
5.96 ± 0.624
5.601 ± 0.406
1.001 ± 0.107
4.229 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here