Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Haemophilus; Haemophilus influenzae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1707 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P44565|MENE_HAEIN 2-succinylbenzoate--CoA ligase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=menE PE=3 SV=1
MM1 pKa = 7.65AIVGLFYY8 pKa = 11.01GSDD11 pKa = 3.2TGNTEE16 pKa = 4.38NIAKK20 pKa = 10.03QIQKK24 pKa = 9.96QLGSDD29 pKa = 4.42LIDD32 pKa = 3.33IRR34 pKa = 11.84DD35 pKa = 3.41IAKK38 pKa = 10.31SSKK41 pKa = 10.35EE42 pKa = 3.95DD43 pKa = 3.06IEE45 pKa = 6.2AYY47 pKa = 10.41DD48 pKa = 3.8FLLFGIPTWYY58 pKa = 10.41YY59 pKa = 11.52GEE61 pKa = 4.97AQADD65 pKa = 3.42WDD67 pKa = 4.41DD68 pKa = 4.28FFPTLEE74 pKa = 4.91EE75 pKa = 4.17IDD77 pKa = 4.03FTDD80 pKa = 3.28KK81 pKa = 11.07LVGIFGCGDD90 pKa = 3.18QEE92 pKa = 5.04DD93 pKa = 4.44YY94 pKa = 11.9ADD96 pKa = 4.18YY97 pKa = 10.87FCDD100 pKa = 4.7AIGTVRR106 pKa = 11.84DD107 pKa = 3.56IIEE110 pKa = 3.76PHH112 pKa = 5.69GAIVVGNWPTEE123 pKa = 3.97GYY125 pKa = 10.55NFEE128 pKa = 4.07ASKK131 pKa = 11.28ALLEE135 pKa = 5.01DD136 pKa = 3.46GTFIGLCIDD145 pKa = 3.99EE146 pKa = 5.02DD147 pKa = 3.94RR148 pKa = 11.84QPEE151 pKa = 4.14LTAEE155 pKa = 4.58RR156 pKa = 11.84VEE158 pKa = 4.35KK159 pKa = 9.58WCKK162 pKa = 9.65QIYY165 pKa = 10.55DD166 pKa = 4.07EE167 pKa = 4.49MCLAEE172 pKa = 4.39LAA174 pKa = 4.24

Molecular weight: 19.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P71350|THIE_HAEIN Thiamine-phosphate synthase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=thiE PE=1 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1705

2

1707

528345

21

1694

309.5

34.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.204 ± 0.065

1.032 ± 0.019

4.988 ± 0.042

6.486 ± 0.063

4.466 ± 0.042

6.653 ± 0.055

2.052 ± 0.024

7.081 ± 0.055

6.319 ± 0.052

10.52 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.03

4.896 ± 0.06

3.718 ± 0.033

4.657 ± 0.049

4.47 ± 0.054

5.856 ± 0.044

5.211 ± 0.039

6.667 ± 0.045

1.147 ± 0.023

3.146 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski