Photobacterium damselae subsp. damselae CIP 102761
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3498 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D0Z4Q9|D0Z4Q9_PHODD Inosine-uridine preferring nucleoside hydrolase OS=Photobacterium damselae subsp. damselae CIP 102761 OX=675817 GN=VDA_000517 PE=4 SV=1
MM1 pKa = 7.31 NVFKK5 pKa = 11.07 GIAKK9 pKa = 10.21 VGITAVASISFAAMALTEE27 pKa = 4.39 SGTVIKK33 pKa = 10.3 NQAGATYY40 pKa = 9.91 RR41 pKa = 11.84 DD42 pKa = 3.47 SANILRR48 pKa = 11.84 STTSNLVEE56 pKa = 4.21 TLVQPVAALTLINDD70 pKa = 3.68 QSKK73 pKa = 10.8 LGAPGRR79 pKa = 11.84 TVTFNHH85 pKa = 5.99 VLTNTGNSTDD95 pKa = 3.66 SFALDD100 pKa = 3.4 MVLPASVISSATIYY114 pKa = 10.49 PDD116 pKa = 3.44 TNNDD120 pKa = 2.91 GVADD124 pKa = 4.41 PGSTPIASGDD134 pKa = 3.64 VVGPLAPGEE143 pKa = 4.19 SFEE146 pKa = 4.27 FVIVADD152 pKa = 4.2 IDD154 pKa = 3.62 PAATINSSNALTITATSQAVVVDD177 pKa = 4.86 GVAVANGGVSAQTNTDD193 pKa = 3.0 TVTATNLPIVEE204 pKa = 4.09 ITKK207 pKa = 10.5 ALSSSEE213 pKa = 4.07 GASPSGPYY221 pKa = 9.1 TVTFTYY227 pKa = 10.68 TNVGLTDD234 pKa = 3.89 MDD236 pKa = 4.33 PLQTNGVILNDD247 pKa = 3.52 VLPEE251 pKa = 3.81 GMRR254 pKa = 11.84 FNGAVSWSISGTTITASGTTTGGSEE279 pKa = 4.05 TSGDD283 pKa = 3.15 QDD285 pKa = 4.74 LAFTTCIAPDD295 pKa = 4.01 ASCPNNDD302 pKa = 3.58 EE303 pKa = 4.22 IQFVMDD309 pKa = 4.52 GLASDD314 pKa = 4.65 EE315 pKa = 4.29 SATISFEE322 pKa = 4.24 VNIDD326 pKa = 3.56 SGLSATTLYY335 pKa = 11.01 NQGTYY340 pKa = 10.98 GFDD343 pKa = 3.45 EE344 pKa = 5.2 DD345 pKa = 4.25 NSGTVDD351 pKa = 3.54 VGEE354 pKa = 4.0 QDD356 pKa = 3.12 STNTNRR362 pKa = 11.84 VPFLITSNYY371 pKa = 9.76 AVIANNGGCDD381 pKa = 2.94 VGTDD385 pKa = 3.88 NNCDD389 pKa = 3.33 GSDD392 pKa = 3.79 DD393 pKa = 4.05 TNHH396 pKa = 6.92 EE397 pKa = 4.13 IVDD400 pKa = 3.73 IASASQGARR409 pKa = 11.84 VSFTNYY415 pKa = 8.22 IWNTGNDD422 pKa = 3.08 EE423 pKa = 5.01 DD424 pKa = 4.64 IFNISIEE431 pKa = 4.22 SSTFPSGTSFFLYY444 pKa = 10.26 KK445 pKa = 10.15 SDD447 pKa = 3.82 GVTPLIDD454 pKa = 3.68 TDD456 pKa = 3.7 NDD458 pKa = 4.22 SNPDD462 pKa = 3.35 TGVIPAAGEE471 pKa = 4.09 TCPSYY476 pKa = 11.15 LVTDD480 pKa = 4.81 GNLCGYY486 pKa = 10.1 KK487 pKa = 9.96 VVLVATLPASALGGPYY503 pKa = 10.12 QITKK507 pKa = 10.21 RR508 pKa = 11.84 ATSSSDD514 pKa = 2.93 TSKK517 pKa = 10.1 TNTVIDD523 pKa = 3.74 RR524 pKa = 11.84 LGTITASTVDD534 pKa = 3.35 LTNNFRR540 pKa = 11.84 AEE542 pKa = 3.9 TSLTVEE548 pKa = 4.85 DD549 pKa = 4.39 SCDD552 pKa = 3.72 AADD555 pKa = 4.34 DD556 pKa = 3.94 NCGIGAGPEE565 pKa = 4.04 ATPVTTNTTAPGTTTRR581 pKa = 11.84 FTLYY585 pKa = 8.97 VTNTSGAADD594 pKa = 3.86 SYY596 pKa = 10.2 EE597 pKa = 3.95 LQYY600 pKa = 11.45 SSTNFAEE607 pKa = 4.24 NTMPADD613 pKa = 3.14 WSVVFKK619 pKa = 10.96 NAAGEE624 pKa = 4.36 VITNIPVLGPDD635 pKa = 3.78 DD636 pKa = 4.84 AMLVYY641 pKa = 10.93 ADD643 pKa = 3.4 VTVPEE648 pKa = 4.3 NATTATQDD656 pKa = 3.13 VYY658 pKa = 11.22 FKK660 pKa = 10.91 VVSPTTGASDD670 pKa = 3.63 IKK672 pKa = 10.59 RR673 pKa = 11.84 DD674 pKa = 3.47 AVTIDD679 pKa = 3.73 NSSQCLAFGPPNANGVTYY697 pKa = 10.58 AGGSIVYY704 pKa = 9.34 KK705 pKa = 10.52 HH706 pKa = 6.25 ILNNTSNDD714 pKa = 3.11 AYY716 pKa = 10.04 TNIALTVTNSDD727 pKa = 3.06 SDD729 pKa = 3.86 FSAVIYY735 pKa = 10.52 EE736 pKa = 4.37 DD737 pKa = 3.79 TNGDD741 pKa = 3.84 DD742 pKa = 3.98 VLTGADD748 pKa = 3.48 NSITSIASLAGGAQKK763 pKa = 11.08 VLFVKK768 pKa = 10.47 VLAPANASQGQDD780 pKa = 2.71 NITTVEE786 pKa = 4.16 TTVACGTLQIIDD798 pKa = 3.8 TTTIANTNMKK808 pKa = 8.2 VTKK811 pKa = 9.85 EE812 pKa = 3.87 QTPDD816 pKa = 3.26 LDD818 pKa = 4.55 CNGTSDD824 pKa = 3.28 TGNFVTTNFAVAPGEE839 pKa = 4.19 CIIYY843 pKa = 10.66 RR844 pKa = 11.84 LTTEE848 pKa = 4.57 NIAIEE853 pKa = 4.37 DD854 pKa = 3.67 AHH856 pKa = 6.58 NVTLTDD862 pKa = 3.32 ATPAYY867 pKa = 6.73 TTFNVTGGIPSITEE881 pKa = 3.66 GTINTVINGTTGSIIGTVGTVAPGEE906 pKa = 4.32 SEE908 pKa = 3.94 TLIFGIRR915 pKa = 11.84 IDD917 pKa = 3.57 NN918 pKa = 3.72
Molecular weight: 94.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.656
IPC_protein 3.706
Toseland 3.465
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.516
Grimsley 3.376
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.821
Patrickios 0.935
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|D0Z141|D0Z141_PHODD GDT1 family protein OS=Photobacterium damselae subsp. damselae CIP 102761 OX=675817 GN=VDA_003255 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 8.98 RR4 pKa = 11.84 TFQPSVLKK12 pKa = 10.49 RR13 pKa = 11.84 KK14 pKa = 7.97 RR15 pKa = 11.84 THH17 pKa = 5.89 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.41 NGRR29 pKa = 11.84 AVINARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.74 GRR40 pKa = 11.84 KK41 pKa = 8.91 RR42 pKa = 11.84 LSKK45 pKa = 10.84
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3498
0
3498
1276815
37
7833
365.0
40.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.464 ± 0.05
1.067 ± 0.017
5.63 ± 0.07
5.927 ± 0.041
4.041 ± 0.031
6.736 ± 0.062
2.311 ± 0.025
6.695 ± 0.034
5.343 ± 0.061
10.196 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.613 ± 0.029
4.526 ± 0.04
3.985 ± 0.03
4.902 ± 0.042
4.386 ± 0.043
6.385 ± 0.039
5.623 ± 0.062
6.726 ± 0.043
1.24 ± 0.017
3.202 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here