Amino acid dipepetide frequency for Cycloclasticus sp. symbiont of Poecilosclerida sp. M

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.615AlaAla: 7.615 ± 0.153
1.246AlaCys: 1.246 ± 0.05
4.78AlaAsp: 4.78 ± 0.111
5.474AlaGlu: 5.474 ± 0.106
3.425AlaPhe: 3.425 ± 0.088
6.453AlaGly: 6.453 ± 0.117
1.636AlaHis: 1.636 ± 0.055
5.83AlaIle: 5.83 ± 0.114
4.999AlaLys: 4.999 ± 0.094
9.375AlaLeu: 9.375 ± 0.143
2.271AlaMet: 2.271 ± 0.068
3.108AlaAsn: 3.108 ± 0.07
2.719AlaPro: 2.719 ± 0.068
3.137AlaGln: 3.137 ± 0.086
3.703AlaArg: 3.703 ± 0.085
5.465AlaSer: 5.465 ± 0.1
4.387AlaThr: 4.387 ± 0.087
6.008AlaVal: 6.008 ± 0.113
0.969AlaTrp: 0.969 ± 0.042
2.328AlaTyr: 2.328 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
0.905CysAla: 0.905 ± 0.045
0.207CysCys: 0.207 ± 0.021
0.732CysAsp: 0.732 ± 0.037
0.72CysGlu: 0.72 ± 0.037
0.486CysPhe: 0.486 ± 0.031
1.028CysGly: 1.028 ± 0.046
0.373CysHis: 0.373 ± 0.027
0.784CysIle: 0.784 ± 0.039
0.6CysLys: 0.6 ± 0.035
1.182CysLeu: 1.182 ± 0.052
0.279CysMet: 0.279 ± 0.023
0.446CysAsn: 0.446 ± 0.028
0.549CysPro: 0.549 ± 0.039
0.468CysGln: 0.468 ± 0.03
0.54CysArg: 0.54 ± 0.031
0.9CysSer: 0.9 ± 0.043
0.633CysThr: 0.633 ± 0.043
0.916CysVal: 0.916 ± 0.042
0.149CysTrp: 0.149 ± 0.017
0.374CysTyr: 0.374 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
4.806AspAla: 4.806 ± 0.116
0.665AspCys: 0.665 ± 0.042
3.295AspAsp: 3.295 ± 0.087
4.395AspGlu: 4.395 ± 0.094
2.443AspPhe: 2.443 ± 0.074
3.791AspGly: 3.791 ± 0.094
1.017AspHis: 1.017 ± 0.056
4.081AspIle: 4.081 ± 0.083
3.411AspLys: 3.411 ± 0.091
5.28AspLeu: 5.28 ± 0.099
1.399AspMet: 1.399 ± 0.049
2.174AspAsn: 2.174 ± 0.059
2.034AspPro: 2.034 ± 0.052
1.621AspGln: 1.621 ± 0.047
2.175AspArg: 2.175 ± 0.066
3.266AspSer: 3.266 ± 0.077
2.796AspThr: 2.796 ± 0.07
4.408AspVal: 4.408 ± 0.096
0.802AspTrp: 0.802 ± 0.038
1.845AspTyr: 1.845 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
5.322GluAla: 5.322 ± 0.114
0.587GluCys: 0.587 ± 0.034
3.044GluAsp: 3.044 ± 0.075
4.195GluGlu: 4.195 ± 0.107
2.322GluPhe: 2.322 ± 0.066
4.307GluGly: 4.307 ± 0.094
1.59GluHis: 1.59 ± 0.059
4.167GluIle: 4.167 ± 0.09
4.699GluLys: 4.699 ± 0.122
6.612GluLeu: 6.612 ± 0.118
1.683GluMet: 1.683 ± 0.053
2.662GluAsn: 2.662 ± 0.07
2.06GluPro: 2.06 ± 0.063
3.528GluGln: 3.528 ± 0.092
3.332GluArg: 3.332 ± 0.094
3.816GluSer: 3.816 ± 0.097
3.38GluThr: 3.38 ± 0.078
4.316GluVal: 4.316 ± 0.104
0.687GluTrp: 0.687 ± 0.033
1.672GluTyr: 1.672 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.025PheAla: 3.025 ± 0.081
0.54PheCys: 0.54 ± 0.031
2.629PheAsp: 2.629 ± 0.067
2.52PheGlu: 2.52 ± 0.064
1.799PhePhe: 1.799 ± 0.07
2.987PheGly: 2.987 ± 0.082
0.844PheHis: 0.844 ± 0.037
2.693PheIle: 2.693 ± 0.071
2.285PheLys: 2.285 ± 0.062
3.782PheLeu: 3.782 ± 0.095
0.944PheMet: 0.944 ± 0.046
1.9PheAsn: 1.9 ± 0.063
1.503PhePro: 1.503 ± 0.05
1.235PheGln: 1.235 ± 0.048
1.548PheArg: 1.548 ± 0.053
3.358PheSer: 3.358 ± 0.078
2.115PheThr: 2.115 ± 0.057
2.629PheVal: 2.629 ± 0.07
0.43PheTrp: 0.43 ± 0.031
1.272PheTyr: 1.272 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
5.61GlyAla: 5.61 ± 0.107
1.006GlyCys: 1.006 ± 0.047
3.93GlyAsp: 3.93 ± 0.088
4.733GlyGlu: 4.733 ± 0.098
3.337GlyPhe: 3.337 ± 0.079
5.469GlyGly: 5.469 ± 0.138
1.775GlyHis: 1.775 ± 0.054
4.692GlyIle: 4.692 ± 0.115
4.382GlyLys: 4.382 ± 0.092
7.611GlyLeu: 7.611 ± 0.124
2.078GlyMet: 2.078 ± 0.076
2.44GlyAsn: 2.44 ± 0.071
1.869GlyPro: 1.869 ± 0.065
2.522GlyGln: 2.522 ± 0.065
3.341GlyArg: 3.341 ± 0.079
4.222GlySer: 4.222 ± 0.1
3.532GlyThr: 3.532 ± 0.093
5.656GlyVal: 5.656 ± 0.126
0.938GlyTrp: 0.938 ± 0.043
2.162GlyTyr: 2.162 ± 0.071
0.0GlyXaa: 0.0 ± 0.0
His
1.887HisAla: 1.887 ± 0.06
0.336HisCys: 0.336 ± 0.025
1.158HisAsp: 1.158 ± 0.057
1.142HisGlu: 1.142 ± 0.046
1.035HisPhe: 1.035 ± 0.048
1.667HisGly: 1.667 ± 0.052
0.652HisHis: 0.652 ± 0.039
1.437HisIle: 1.437 ± 0.049
1.19HisLys: 1.19 ± 0.041
2.243HisLeu: 2.243 ± 0.067
0.575HisMet: 0.575 ± 0.032
0.9HisAsn: 0.9 ± 0.037
1.186HisPro: 1.186 ± 0.051
1.035HisGln: 1.035 ± 0.045
1.037HisArg: 1.037 ± 0.043
1.289HisSer: 1.289 ± 0.053
1.191HisThr: 1.191 ± 0.05
1.516HisVal: 1.516 ± 0.052
0.334HisTrp: 0.334 ± 0.027
0.8HisTyr: 0.8 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
6.001IleAla: 6.001 ± 0.124
0.824IleCys: 0.824 ± 0.042
4.393IleAsp: 4.393 ± 0.103
4.575IleGlu: 4.575 ± 0.113
2.324IlePhe: 2.324 ± 0.074
4.744IleGly: 4.744 ± 0.101
1.303IleHis: 1.303 ± 0.058
4.211IleIle: 4.211 ± 0.104
4.356IleLys: 4.356 ± 0.087
5.463IleLeu: 5.463 ± 0.118
1.358IleMet: 1.358 ± 0.054
3.121IleAsn: 3.121 ± 0.074
2.798IlePro: 2.798 ± 0.064
2.41IleGln: 2.41 ± 0.07
2.987IleArg: 2.987 ± 0.076
4.527IleSer: 4.527 ± 0.099
3.774IleThr: 3.774 ± 0.08
4.356IleVal: 4.356 ± 0.091
0.554IleTrp: 0.554 ± 0.034
1.678IleTyr: 1.678 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
5.113LysAla: 5.113 ± 0.105
0.503LysCys: 0.503 ± 0.037
2.985LysAsp: 2.985 ± 0.079
4.061LysGlu: 4.061 ± 0.109
1.663LysPhe: 1.663 ± 0.057
4.092LysGly: 4.092 ± 0.093
1.403LysHis: 1.403 ± 0.052
3.793LysIle: 3.793 ± 0.074
4.643LysLys: 4.643 ± 0.114
5.783LysLeu: 5.783 ± 0.096
1.535LysMet: 1.535 ± 0.053
2.851LysAsn: 2.851 ± 0.072
2.473LysPro: 2.473 ± 0.063
3.009LysGln: 3.009 ± 0.082
3.247LysArg: 3.247 ± 0.077
3.62LysSer: 3.62 ± 0.08
3.717LysThr: 3.717 ± 0.086
3.851LysVal: 3.851 ± 0.079
0.542LysTrp: 0.542 ± 0.035
1.364LysTyr: 1.364 ± 0.055
0.0LysXaa: 0.0 ± 0.0
Leu
9.304LeuAla: 9.304 ± 0.151
1.186LeuCys: 1.186 ± 0.053
5.805LeuAsp: 5.805 ± 0.122
6.374LeuGlu: 6.374 ± 0.109
3.93LeuPhe: 3.93 ± 0.096
7.119LeuGly: 7.119 ± 0.116
2.216LeuHis: 2.216 ± 0.061
6.603LeuIle: 6.603 ± 0.124
6.188LeuLys: 6.188 ± 0.119
10.578LeuLeu: 10.578 ± 0.196
2.743LeuMet: 2.743 ± 0.062
4.42LeuAsn: 4.42 ± 0.099
4.395LeuPro: 4.395 ± 0.104
3.769LeuGln: 3.769 ± 0.085
4.457LeuArg: 4.457 ± 0.096
7.89LeuSer: 7.89 ± 0.16
5.498LeuThr: 5.498 ± 0.113
6.728LeuVal: 6.728 ± 0.118
0.934LeuTrp: 0.934 ± 0.047
2.621LeuTyr: 2.621 ± 0.069
0.0LeuXaa: 0.0 ± 0.0
Met
2.425MetAla: 2.425 ± 0.072
0.275MetCys: 0.275 ± 0.021
1.307MetAsp: 1.307 ± 0.057
1.369MetGlu: 1.369 ± 0.055
0.894MetPhe: 0.894 ± 0.043
1.894MetGly: 1.894 ± 0.059
0.562MetHis: 0.562 ± 0.033
1.314MetIle: 1.314 ± 0.052
1.513MetLys: 1.513 ± 0.051
2.765MetLeu: 2.765 ± 0.076
0.665MetMet: 0.665 ± 0.033
1.072MetAsn: 1.072 ± 0.05
1.268MetPro: 1.268 ± 0.048
1.12MetGln: 1.12 ± 0.045
1.257MetArg: 1.257 ± 0.048
2.021MetSer: 2.021 ± 0.069
1.472MetThr: 1.472 ± 0.051
1.825MetVal: 1.825 ± 0.059
0.198MetTrp: 0.198 ± 0.019
0.553MetTyr: 0.553 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
3.403AsnAla: 3.403 ± 0.074
0.497AsnCys: 0.497 ± 0.028
2.082AsnAsp: 2.082 ± 0.059
2.522AsnGlu: 2.522 ± 0.072
1.465AsnPhe: 1.465 ± 0.048
2.785AsnGly: 2.785 ± 0.079
0.859AsnHis: 0.859 ± 0.041
3.053AsnIle: 3.053 ± 0.079
2.675AsnLys: 2.675 ± 0.074
3.824AsnLeu: 3.824 ± 0.089
1.05AsnMet: 1.05 ± 0.052
1.805AsnAsn: 1.805 ± 0.067
2.084AsnPro: 2.084 ± 0.071
1.564AsnGln: 1.564 ± 0.058
1.863AsnArg: 1.863 ± 0.061
2.41AsnSer: 2.41 ± 0.073
2.287AsnThr: 2.287 ± 0.071
2.64AsnVal: 2.64 ± 0.064
0.51AsnTrp: 0.51 ± 0.027
1.168AsnTyr: 1.168 ± 0.048
0.0AsnXaa: 0.0 ± 0.0
Pro
3.079ProAla: 3.079 ± 0.073
0.426ProCys: 0.426 ± 0.027
2.361ProAsp: 2.361 ± 0.07
3.033ProGlu: 3.033 ± 0.078
1.617ProPhe: 1.617 ± 0.057
2.385ProGly: 2.385 ± 0.079
0.982ProHis: 0.982 ± 0.045
2.688ProIle: 2.688 ± 0.075
2.109ProLys: 2.109 ± 0.066
4.002ProLeu: 4.002 ± 0.089
1.083ProMet: 1.083 ± 0.04
1.689ProAsn: 1.689 ± 0.055
1.386ProPro: 1.386 ± 0.053
1.351ProGln: 1.351 ± 0.055
1.529ProArg: 1.529 ± 0.055
2.665ProSer: 2.665 ± 0.068
2.15ProThr: 2.15 ± 0.06
3.036ProVal: 3.036 ± 0.079
0.433ProTrp: 0.433 ± 0.029
1.151ProTyr: 1.151 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
3.912GlnAla: 3.912 ± 0.101
0.444GlnCys: 0.444 ± 0.027
1.704GlnAsp: 1.704 ± 0.057
2.252GlnGlu: 2.252 ± 0.06
1.544GlnPhe: 1.544 ± 0.05
2.434GlnGly: 2.434 ± 0.075
1.114GlnHis: 1.114 ± 0.047
2.337GlnIle: 2.337 ± 0.067
2.227GlnLys: 2.227 ± 0.067
4.54GlnLeu: 4.54 ± 0.114
0.964GlnMet: 0.964 ± 0.046
1.448GlnAsn: 1.448 ± 0.057
1.456GlnPro: 1.456 ± 0.049
2.493GlnGln: 2.493 ± 0.075
2.214GlnArg: 2.214 ± 0.07
2.285GlnSer: 2.285 ± 0.065
2.201GlnThr: 2.201 ± 0.071
2.831GlnVal: 2.831 ± 0.067
0.525GlnTrp: 0.525 ± 0.039
1.087GlnTyr: 1.087 ± 0.046
0.0GlnXaa: 0.0 ± 0.0
Arg
3.541ArgAla: 3.541 ± 0.079
0.571ArgCys: 0.571 ± 0.03
2.462ArgAsp: 2.462 ± 0.073
3.08ArgGlu: 3.08 ± 0.086
2.095ArgPhe: 2.095 ± 0.067
3.088ArgGly: 3.088 ± 0.076
1.048ArgHis: 1.048 ± 0.046
3.047ArgIle: 3.047 ± 0.072
2.592ArgLys: 2.592 ± 0.083
4.964ArgLeu: 4.964 ± 0.103
1.252ArgMet: 1.252 ± 0.048
1.727ArgAsn: 1.727 ± 0.062
1.788ArgPro: 1.788 ± 0.063
2.005ArgGln: 2.005 ± 0.07
2.509ArgArg: 2.509 ± 0.079
2.614ArgSer: 2.614 ± 0.072
2.142ArgThr: 2.142 ± 0.067
3.508ArgVal: 3.508 ± 0.09
0.619ArgTrp: 0.619 ± 0.037
1.549ArgTyr: 1.549 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
5.5SerAla: 5.5 ± 0.092
0.885SerCys: 0.885 ± 0.047
3.563SerAsp: 3.563 ± 0.082
3.862SerGlu: 3.862 ± 0.092
2.91SerPhe: 2.91 ± 0.076
5.12SerGly: 5.12 ± 0.099
1.459SerHis: 1.459 ± 0.051
4.43SerIle: 4.43 ± 0.097
3.488SerLys: 3.488 ± 0.089
6.831SerLeu: 6.831 ± 0.119
1.825SerMet: 1.825 ± 0.063
2.565SerAsn: 2.565 ± 0.08
2.59SerPro: 2.59 ± 0.069
2.425SerGln: 2.425 ± 0.066
2.926SerArg: 2.926 ± 0.086
4.529SerSer: 4.529 ± 0.095
3.559SerThr: 3.559 ± 0.089
4.6SerVal: 4.6 ± 0.105
0.727SerTrp: 0.727 ± 0.039
1.814SerTyr: 1.814 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
4.604ThrAla: 4.604 ± 0.099
0.624ThrCys: 0.624 ± 0.034
3.009ThrAsp: 3.009 ± 0.083
2.965ThrGlu: 2.965 ± 0.074
2.032ThrPhe: 2.032 ± 0.066
4.463ThrGly: 4.463 ± 0.091
1.379ThrHis: 1.379 ± 0.049
3.424ThrIle: 3.424 ± 0.081
2.761ThrLys: 2.761 ± 0.077
6.016ThrLeu: 6.016 ± 0.125
1.186ThrMet: 1.186 ± 0.052
1.933ThrAsn: 1.933 ± 0.06
2.667ThrPro: 2.667 ± 0.077
2.126ThrGln: 2.126 ± 0.062
2.377ThrArg: 2.377 ± 0.072
3.392ThrSer: 3.392 ± 0.082
3.102ThrThr: 3.102 ± 0.082
3.712ThrVal: 3.712 ± 0.085
0.569ThrTrp: 0.569 ± 0.03
1.311ThrTyr: 1.311 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
5.786ValAla: 5.786 ± 0.121
0.894ValCys: 0.894 ± 0.044
4.364ValAsp: 4.364 ± 0.099
4.696ValGlu: 4.696 ± 0.114
2.974ValPhe: 2.974 ± 0.075
4.859ValGly: 4.859 ± 0.115
1.45ValHis: 1.45 ± 0.051
4.92ValIle: 4.92 ± 0.094
4.048ValLys: 4.048 ± 0.092
7.279ValLeu: 7.279 ± 0.134
1.924ValMet: 1.924 ± 0.067
2.959ValAsn: 2.959 ± 0.076
2.618ValPro: 2.618 ± 0.07
2.229ValGln: 2.229 ± 0.072
3.08ValArg: 3.08 ± 0.087
4.811ValSer: 4.811 ± 0.095
3.822ValThr: 3.822 ± 0.084
5.425ValVal: 5.425 ± 0.117
0.709ValTrp: 0.709 ± 0.04
1.749ValTyr: 1.749 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.835TrpAla: 0.835 ± 0.038
0.156TrpCys: 0.156 ± 0.016
0.6TrpAsp: 0.6 ± 0.035
0.549TrpGlu: 0.549 ± 0.033
0.512TrpPhe: 0.512 ± 0.033
0.661TrpGly: 0.661 ± 0.036
0.281TrpHis: 0.281 ± 0.021
0.545TrpIle: 0.545 ± 0.033
0.593TrpLys: 0.593 ± 0.034
1.447TrpLeu: 1.447 ± 0.051
0.297TrpMet: 0.297 ± 0.026
0.393TrpAsn: 0.393 ± 0.029
0.475TrpPro: 0.475 ± 0.028
0.619TrpGln: 0.619 ± 0.034
0.628TrpArg: 0.628 ± 0.037
0.76TrpSer: 0.76 ± 0.04
0.47TrpThr: 0.47 ± 0.033
0.863TrpVal: 0.863 ± 0.04
0.167TrpTrp: 0.167 ± 0.017
0.312TrpTyr: 0.312 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.256TyrAla: 2.256 ± 0.062
0.398TyrCys: 0.398 ± 0.03
1.5TyrAsp: 1.5 ± 0.062
1.465TyrGlu: 1.465 ± 0.048
1.228TyrPhe: 1.228 ± 0.048
1.962TyrGly: 1.962 ± 0.069
0.668TyrHis: 0.668 ± 0.036
1.709TyrIle: 1.709 ± 0.055
1.487TyrLys: 1.487 ± 0.054
3.183TyrLeu: 3.183 ± 0.085
0.661TyrMet: 0.661 ± 0.038
0.934TyrAsn: 0.934 ± 0.042
1.237TyrPro: 1.237 ± 0.056
1.346TyrGln: 1.346 ± 0.052
1.45TyrArg: 1.45 ± 0.062
1.766TyrSer: 1.766 ± 0.063
1.421TyrThr: 1.421 ± 0.049
1.771TyrVal: 1.771 ± 0.049
0.367TyrTrp: 0.367 ± 0.028
0.89TyrTyr: 0.89 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1993 proteins (544741 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski