Achlya hypogyna
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14400 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V9Y4U3|A0A1V9Y4U3_9STRA Uncharacterized protein OS=Achlya hypogyna OX=1202772 GN=ACHHYP_17267 PE=4 SV=1
MM1 pKa = 7.17 VFLPFVLLALSGVGAAGIALRR22 pKa = 11.84 SCCYY26 pKa = 10.22 KK27 pKa = 10.75 PEE29 pKa = 4.43 DD30 pKa = 3.48 EE31 pKa = 4.51 WRR33 pKa = 11.84 DD34 pKa = 3.52 PRR36 pKa = 11.84 QEE38 pKa = 4.26 PLMEE42 pKa = 4.84 APNVPATTKK51 pKa = 10.75 ASAPSVTPTLAPPPVVKK68 pKa = 10.62 SSTPPPTAVVKK79 pKa = 10.37 PLDD82 pKa = 4.1 GVGGADD88 pKa = 3.53 PMVEE92 pKa = 3.87 PVACRR97 pKa = 11.84 GCGATIDD104 pKa = 4.04 PSLAALANSLCIVCSYY120 pKa = 10.88 QSIRR124 pKa = 11.84 LPSLEE129 pKa = 4.5 IEE131 pKa = 4.42 QPDD134 pKa = 3.74 DD135 pKa = 3.44 TFPEE139 pKa = 4.55 YY140 pKa = 10.18 IANDD144 pKa = 4.03 DD145 pKa = 4.83 ADD147 pKa = 5.43 DD148 pKa = 4.75 DD149 pKa = 4.25 MDD151 pKa = 4.41 GAGYY155 pKa = 10.41 DD156 pKa = 4.12 DD157 pKa = 5.53 DD158 pKa = 3.44 MSYY161 pKa = 11.2 YY162 pKa = 10.73 YY163 pKa = 10.28 KK164 pKa = 10.91 DD165 pKa = 3.21 KK166 pKa = 11.34 ARR168 pKa = 11.84 TTDD171 pKa = 3.04 EE172 pKa = 4.01 GTTAEE177 pKa = 4.37 MEE179 pKa = 4.47 SPQDD183 pKa = 3.92 LDD185 pKa = 5.82 DD186 pKa = 5.16 GALDD190 pKa = 6.08 DD191 pKa = 6.11 DD192 pKa = 5.58 DD193 pKa = 6.19 EE194 pKa = 7.59 DD195 pKa = 4.57 DD196 pKa = 5.21 ASSLSQEE203 pKa = 4.1 MEE205 pKa = 3.98 IPCADD210 pKa = 3.92 GEE212 pKa = 4.28 PAADD216 pKa = 3.79 EE217 pKa = 5.0 DD218 pKa = 4.47 IEE220 pKa = 4.26 IAEE223 pKa = 4.21 EE224 pKa = 3.97 ALALVQDD231 pKa = 3.99 MWDD234 pKa = 3.05 IAYY237 pKa = 9.06 QAHH240 pKa = 6.91 LGAGEE245 pKa = 4.66 GPDD248 pKa = 2.94 GDD250 pKa = 4.49 AFVEE254 pKa = 4.3 LALEE258 pKa = 4.51 LDD260 pKa = 3.42 ATAAAIVKK268 pKa = 9.3 EE269 pKa = 3.96 PHH271 pKa = 6.21 LLSEE275 pKa = 4.53 SFHH278 pKa = 6.65 FLSQALASLLEE289 pKa = 4.3 LVPEE293 pKa = 4.54 AWAPHH298 pKa = 5.25 VEE300 pKa = 3.87 ATEE303 pKa = 4.26 LKK305 pKa = 10.69 FEE307 pKa = 4.38 ALQFRR312 pKa = 11.84 YY313 pKa = 9.46 HH314 pKa = 6.67 SKK316 pKa = 9.31 LTVEE320 pKa = 4.89 CGLDD324 pKa = 3.45 LAAQLYY330 pKa = 9.81 EE331 pKa = 4.16 LVEE334 pKa = 4.36 CASDD338 pKa = 3.73 FGVDD342 pKa = 3.37 PTVAASVLQGLEE354 pKa = 3.96 EE355 pKa = 3.77 LAGAVEE361 pKa = 4.26 EE362 pKa = 4.89 TPCEE366 pKa = 3.84 LVSWLNYY373 pKa = 10.19 LGATIKK379 pKa = 10.6 LLKK382 pKa = 10.19 SYY384 pKa = 9.85 EE385 pKa = 4.06 RR386 pKa = 11.84 EE387 pKa = 4.07 FEE389 pKa = 5.79 DD390 pKa = 3.6 VAKK393 pKa = 10.2 WDD395 pKa = 3.99 DD396 pKa = 3.62 VVDD399 pKa = 4.26 CEE401 pKa = 4.7 RR402 pKa = 11.84 HH403 pKa = 5.72 LEE405 pKa = 3.89 PLQLHH410 pKa = 6.15 CWEE413 pKa = 5.16 IYY415 pKa = 10.2 SPCC418 pKa = 4.06
Molecular weight: 45.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.088
Thurlkill 3.63
EMBOSS 3.694
Sillero 3.923
Patrickios 0.858
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.827
Protein with the highest isoelectric point:
>tr|A0A1V9YF75|A0A1V9YF75_9STRA Polycystin Cation Channel (PCC) Family OS=Achlya hypogyna OX=1202772 GN=ACHHYP_13415 PE=4 SV=1
MM1 pKa = 7.97 PSPFPTSRR9 pKa = 11.84 RR10 pKa = 11.84 YY11 pKa = 9.43 NVSAVEE17 pKa = 3.97 ALCVLLNRR25 pKa = 11.84 LAWPHH30 pKa = 6.44 RR31 pKa = 11.84 LGSMVSHH38 pKa = 7.45 FGRR41 pKa = 11.84 SRR43 pKa = 11.84 EE44 pKa = 4.0 ALSTIFNAALHH55 pKa = 6.38 HH56 pKa = 5.92 INEE59 pKa = 4.2 RR60 pKa = 11.84 FARR63 pKa = 11.84 LLKK66 pKa = 10.06 WDD68 pKa = 4.0 DD69 pKa = 3.33 RR70 pKa = 11.84 RR71 pKa = 11.84 LDD73 pKa = 3.83 GRR75 pKa = 11.84 WMAACAKK82 pKa = 10.01 AIHH85 pKa = 6.64 AKK87 pKa = 9.77 GAPLDD92 pKa = 3.82 SCIGFIDD99 pKa = 3.82 GTVRR103 pKa = 11.84 GICRR107 pKa = 11.84 PKK109 pKa = 10.87 NGVQRR114 pKa = 11.84 AAYY117 pKa = 9.41 NVYY120 pKa = 10.26 KK121 pKa = 10.61 VLQFQPGLTNN131 pKa = 3.62
Molecular weight: 14.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.589
IPC_protein 10.394
Toseland 10.57
ProMoST 10.277
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.891
Rodwell 10.847
Grimsley 10.73
Solomon 10.789
Lehninger 10.76
Nozaki 10.599
DTASelect 10.394
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.613
Patrickios 10.599
IPC_peptide 10.789
IPC2_peptide 9.706
IPC2.peptide.svr19 8.531
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14400
0
14400
7479346
49
12321
519.4
57.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.161 ± 0.03
1.782 ± 0.01
5.538 ± 0.015
5.268 ± 0.022
3.749 ± 0.013
5.858 ± 0.029
2.766 ± 0.011
3.967 ± 0.014
4.353 ± 0.023
10.29 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.358 ± 0.008
2.988 ± 0.013
5.463 ± 0.022
3.768 ± 0.013
5.828 ± 0.023
6.777 ± 0.025
6.24 ± 0.026
7.73 ± 0.018
1.358 ± 0.008
2.757 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here