Lactobacillus phage T25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sukhumvitvirus; Lactobacillus virus T25

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z6BEH8|A0A2Z6BEH8_9CAUD Uncharacterized protein OS=Lactobacillus phage T25 OX=2036055 PE=4 SV=1
MM1 pKa = 7.55FGGEE5 pKa = 4.06VIATFRR11 pKa = 11.84TYY13 pKa = 10.61EE14 pKa = 3.98KK15 pKa = 10.97AEE17 pKa = 3.98EE18 pKa = 4.74FIDD21 pKa = 4.94ASADD25 pKa = 3.64YY26 pKa = 9.66PDD28 pKa = 4.4WWTVPAMTIVEE39 pKa = 4.38VTDD42 pKa = 4.43DD43 pKa = 3.72EE44 pKa = 4.51QQ45 pKa = 5.52

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z6BEJ9|A0A2Z6BEJ9_9CAUD Lysozyme OS=Lactobacillus phage T25 OX=2036055 PE=3 SV=1
MM1 pKa = 7.11VKK3 pKa = 10.12IGIRR7 pKa = 11.84KK8 pKa = 8.65PSWKK12 pKa = 9.95KK13 pKa = 10.73SIAARR18 pKa = 11.84TKK20 pKa = 10.07GKK22 pKa = 10.38LNRR25 pKa = 11.84KK26 pKa = 7.89IKK28 pKa = 10.17RR29 pKa = 11.84AIIPGYY35 pKa = 7.24GQRR38 pKa = 11.84GVGWAHH44 pKa = 7.47PKK46 pKa = 10.02RR47 pKa = 11.84KK48 pKa = 9.49LYY50 pKa = 10.65NKK52 pKa = 9.45AYY54 pKa = 10.45SRR56 pKa = 11.84TTVSVNDD63 pKa = 4.3LLKK66 pKa = 10.52QSNRR70 pKa = 11.84KK71 pKa = 7.98STSTRR76 pKa = 11.84RR77 pKa = 11.84KK78 pKa = 10.21DD79 pKa = 4.06SIVKK83 pKa = 10.5SNGINFGCSGLIGLTVIVVLAIHH106 pKa = 6.7FWPVVLFAAAATGLIFYY123 pKa = 10.87LNLRR127 pKa = 11.84KK128 pKa = 9.67KK129 pKa = 10.12RR130 pKa = 11.84AQAAKK135 pKa = 9.97KK136 pKa = 10.39EE137 pKa = 4.04NDD139 pKa = 2.93ISEE142 pKa = 4.41NEE144 pKa = 4.29AIAKK148 pKa = 8.96LRR150 pKa = 11.84QYY152 pKa = 11.29KK153 pKa = 10.42SLLDD157 pKa = 3.47QGAITQAEE165 pKa = 4.21YY166 pKa = 10.82DD167 pKa = 3.96VKK169 pKa = 10.77KK170 pKa = 11.19GEE172 pKa = 4.32LLHH175 pKa = 6.52IQTDD179 pKa = 3.95ANDD182 pKa = 3.49EE183 pKa = 4.3STDD186 pKa = 5.05DD187 pKa = 3.26SWQDD191 pKa = 3.1FF192 pKa = 3.68

Molecular weight: 21.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

11885

37

1369

237.7

26.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.624 ± 0.501

0.555 ± 0.11

7.135 ± 0.242

4.947 ± 0.312

3.458 ± 0.226

7.244 ± 0.459

1.868 ± 0.168

6.075 ± 0.165

7.547 ± 0.457

7.472 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.827 ± 0.146

5.276 ± 0.286

3.467 ± 0.26

4.544 ± 0.231

3.887 ± 0.381

6.883 ± 0.274

6.176 ± 0.31

6.42 ± 0.251

1.725 ± 0.21

3.87 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski